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Detection and Classification of Porcine Endogenous Retroviruses by Polymerase Chain Reaction

중합효소 연쇄반응을 이용한 돼지 내인성 레트로 바이러스의 검출과 분류

  • Lee, D.H. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University) ;
  • Lee, J.E. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University) ;
  • Kim, H.M. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University) ;
  • Kim, G.W. (Department of Animal Resources Science, College of Industrial Science, Kongju National University) ;
  • Park, H.Y. (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University) ;
  • Kim, Young-Bong (Department of Animal Biotechnology, College of Animal Bioscience & Technology, Konkuk University)
  • 이동희 (건국대학교 동물생명과학대학 동물생명공학과) ;
  • 이정은 (건국대학교 동물생명과학대학 동물생명공학과) ;
  • 김훈미 (건국대학교 동물생명과학대학 동물생명공학과) ;
  • 김계웅 (공주대학교 산업과학대학 동물자원학과) ;
  • 박홍양 (건국대학교 동물생명과학대학 동물생명공학과) ;
  • 김영봉 (건국대학교 동물생명과학대학 동물생명공학과)
  • Published : 2007.06.30

Abstract

Pigs have been considered as an ideal source of donor organs because of their plentiful supply and their numerous anatomical and physiological similarities to the human in xenotransplantation. However, for the public health risks associated with the potential for porcine endogenous retrovirus(PERV) infection through xenograft from pig to human, the investigation of methods for elimination and/or control of PERV has been required. In this study we developed the detection and classification methods for PERV based on PCR using specific primers. PERV-A and PERV-B were found in all pigs including Berkshire, Duroc, Landrace, Yorkshire, miniature pig, and Korean native black pig from Jeju by PCR with type-specific primers for PERV. However, PERV-C was detected only from Duroc, miniature pig, and Korean native black pig from Jeju. PERV-A and PERV-B could be distinguished by PCR-RFLP with BamHI. These methods for PERV will be useful in rapid screening of safe organ for xenograft, furthermore, helpful in monitoring of PERV during and after xenotransplantation.

Keywords

Porcine endogenous retrovirus;Xenotransplantation;Detection;Classification;PCR;RFLP

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