DOI QR코드

DOI QR Code

Expression of Monocarboxylate Transporters(MCTs) and Basigin and Estrogen Receptor α(ERα)-Mediated Regulation of MCT1 Expression in The Efferent Ductules of Male Reproductive Tract During Postnatal Development

생후 발달과정동안 남성 생식기의 Efferent Ductules에서 Monocarboxylate Transporters(MCTs)와 Basigin의 발현 양상과 에스트로젠 수용체 α에 의한 MCT1 발현 조절 연구

  • Lee, Ki-Ho (Department of Biochemistry-Molecular Biology and Center of Antiaging, Eulji University) ;
  • Choi, I.H. (School of Biotechnology, Yeungnam University) ;
  • Chung, C.S. (Department of Animal Science, Chungbuk National University) ;
  • Chang, J.S. (Department of Agricultural Sciences, Korea National Open University)
  • Published : 2007.06.30

Abstract

The purpose of the present study was to determine if monocarboxylate transporter(MCT) isoforms and Basigin(Bsg) are expressed in the efferent ductules(ED) and if MCT1 expression is under estrogen receptor(ER)α-regulation in the ED of male reproductive tract. The presence of MCT isoforms and Bsg mRNAs was detected by real-time polymerization chain reaction(PCR), and ERα-mediated regulation of MCT1 expression in the ED was indirectly determined by immuno- histochemistry. Current study found differential expression of MCT isoforms(MCT1, 2, 3, 4, and 8) and Bsg mRNAs in rat ED according to postnatal ages. In addition, comparison of MCT1 expression in the ED between wild type and ERα knockout mice at different postnatal ages showed basolateral localization of MCT1 in ciliated cells of the ED and, in part, ERα- mediated regulation of MCT1 expression. It is suggested that MCTs would play a role in regulation of function of the ED.

Keywords

Efferent ductules;Monocarboxylate transporter;Basigin;Estrogen receptor $\alpha$;Estrogen

References

  1. Zucker, S., Hymowitz, M., Rollo, E. E., Mann, R., Conner, C. E., Cao, J., Foda, H. D., Tompkins, D. C. and Toole, B. P. 2001. Tumorigenic potential of extracellular matrix metalloproteinase inducer. Am. J. Pathol. 158: 1921-1928 https://doi.org/10.1016/S0002-9440(10)64660-3
  2. Philp, N. J., Ochrietor, J. D., Rudoy, c, Muramatsu, T. and Linser, P. J. 2003. Loss of MCT1, MCT3, and MCT4 expression in the retinal pigment epithelium and neural retina of the 5A11/basigin-null mouse. Invest. Ophthalmol. Vis. Sci. 44: 1305-1311 https://doi.org/10.1167/iovs.02-0552
  3. Wistuba, J., Mittag, J., Luetjens, C. M., Cooper, T. G., Yeung, C. H., Nieschlag, E. and Bauer, K. 2007. Male congenital hypothyroid Pax8-/-mice are infertile: despite adequate treatment with thyroid hormone, J. Endocrinol. 192:99-109 https://doi.org/10.1677/JOE-06-0054
  4. Lee, K. H., Hess, R. A., Bahr, J. M., Lubahn, D. B., Taylor, J. and Bunick, D. 2000. Estrogen rece-ptor u has ill functional role in the mouse rete testis and efferent ductules. Biol. Reprod. 63:18731880
  5. Nakai, M., Chen, L. and Nowak, R. A. 2006. Tissue distribution of basigin and monocarboxylate transporter 1 in the adult male mouse: a study using the wild-type and basigin gene knockout mice. Anat. Rec. A. Discov. Mol. Cell. Evol. BioI. 2:88:527-535
  6. llio, K. and Hess, R. 1994. Structure and function of the ductuli efferentes: A review. Microsc. Res. Tech. 29:432-467 https://doi.org/10.1002/jemt.1070290604
  7. llio, K. and Hess, R. 1994. Structure and function of the ductu1i efferentes: A review. Microsc. Res. Tech. 29:432-467 https://doi.org/10.1002/jemt.1070290604
  8. Kirk, P., Wilson, M. C., HaMle, C., Brown, M. H., Barclay, A. N. and Halestrap, A. P. 2000.CD147 is tightly associated with lactate transporters MCT1 and MCT4 and facilitates their cell surface expression. EMBO J. 19:3896-3904 https://doi.org/10.1093/emboj/19.15.3896
  9. KoehIer-Stoc, E. M., Simpson, I. A., Vannucci, S. J., Landschulz, K. T. lind Landschulz, W. H. 1998. Monocarboxylate transporter expression In mouse brain. Am. J. Physiol. 275:E516..524
  10. Graham, C., Gatherer, I., Haslam, I., Glanville, M. and Simmons, N. L. 2007. Expression and localization of monocarboxylate transporters and sodium/proton exchangers in bovine rumen epithelium. Am. J. Physiol. Regul. Integr. Comp. Physiol. 292:R997-1007
  11. Halestrap, A. P. and Price, N. T. 1999. The protonlinked monocarboxylate transporter (MCT) family: structure, function and regulation. Biochem. J. 343:281-299 https://doi.org/10.1042/0264-6021:3430281
  12. Halestrap, A. P. and Meredith, D. 2004. The SLC16 gene family-free monocarboxylate trans- porters (MCfs) to aromatic amino acid transporters and beyond. Eur. J. Physiol. 447:619-628 https://doi.org/10.1007/s00424-003-1067-2
  13. Heuer, H., Maier, M. K., Iden, S., Mittag, J., Friesema, E. C., Visser, T. J. and Bauer, K. 2005. The monocarboxylate transporter 8 linked to human psychomotor retardation is highly expressed in thyroid hormone-sensitive neuron populations. Endocrinology. 146:1701-1706 https://doi.org/10.1210/en.2004-1179
  14. Cooke, P. S., Young, P., Hess, R. A. and Cunha, G. R. 1991. Estrogen receptor expression in developing epididymis, efferent ductules, and other male reproductive organs. Endocrinology. 128: 2874-2879 https://doi.org/10.1210/endo-128-6-2874
  15. Deora, A. A., Philp, N., Hu, J., Bok, D. and Rodriguez-Boulan, E. 2005. Mechanisms regulating tissue-specific polarity of monocarboxylate transporters and their chaperone CD147 in kidney and retinal epithelia. Proc Natl Acad Sci USA. 102: 16245-16250
  16. Enoki, T., Yoshida, Y., Lally, J., Hatta, H. and Bonen, A. 2006. Testosterone increases lactate transport, monocarboxylate transporter (MCT) 1 and MCT4 in rat skeletal muscle. J. Physiol. 2006. 577:433-443 https://doi.org/10.1113/jphysiol.2006.115436
  17. Friesema, E. C., Ganguly, S., Abdalla, A., Manning, Fox J. E., Halestrap, A. P. and Visser, T. J. 2003. Identification of monocarboxylate transporter 8 as a specific thyroid hormone transporter. J. BioI. Chem. 278:40128-40135 https://doi.org/10.1074/jbc.M300909200
  18. Garcia, C. K., Goldstein, J. L., Pathak, R. K., Anderson, R. G. and Brown, M. S, 1994. Molecular characterization of a membrane transporter for lactate, pyruvate, and other monocarboxylates : implications for the Cori cycle. Cell. 76:865-873 https://doi.org/10.1016/0092-8674(94)90361-1
  19. Gondos, B. and Berndston, W. E. 1993. Postnatal and pubertal development in the sertoli cell, L. D. Russel. and Griswold, M.D. (Eds.), Cache River Press, Clearwater, U.S.A. p.IIS
  20. Branuchi, S., Rauch, M. C, Alfaro, I. E., Cea, C., Concha, II, Benos, D. J. and Reyes, J. G. 2004. Kinetics, molecular basis, and differentiation of L-lactate transport in spermatogenic cells. Am J Physiol Cell Physiol. 288:C523-34 https://doi.org/10.1152/ajpcell.00448.2003
  21. Chen, S., Kadomatsu, K., Kondo, M., Toyama, Y., Toshimori, K., Ueno, S., Miyake, Y. and Muramatsu, T. 2004. Effects of flanking genes on the phenotypes of mice deficient in basigin/ CD 147. Biochem. Biophys. Res. Commun. 324:147-153 https://doi.org/10.1016/j.bbrc.2004.08.232
  22. Clulow, J., Jones, R. and Hansen, L. 1994. Micropuncture and cannulation studies of fluid composition and transport in the ductuli efferentes testis of the rat: comparisons with the homologous metanephric proximal tubule. Exp. Physiol. 79: 915-928 https://doi.org/10.1113/expphysiol.1994.sp003817
  23. Clulow, J., Jones, R. c., Hansen, L. A. and Man, S. Y. 1998. Fluid and electrolyte reabsorption in the ductuli efferentes testes. J. Reprod. Fertil. Suppl. 53:1-4
  24. Garcia, C. K., Brown, M. S., Pathak, R. K. and Goldstein, J. L. 1995. cDNA cloning of MCn, a second monocarboxylate transporter expressed ill different cells than MCT1. J Biol Chem. 270: 1843-1849 https://doi.org/10.1074/jbc.270.4.1843
  25. Rosenfeld, C. S., Ganjam, V. K., Taylor, J. A., Yuan, X., Stiehr, J. R., Hardy, M. P. and Lubahn, D. B. 1998. Transcription and translation of estrogen receptor-beta in the male reproductive tract of estrogen receptor-alpha knock-out and wild-type mice. Endocrinology. 139:2982-2987 https://doi.org/10.1210/en.139.6.2982
  26. Lee, K. H., Finnigan Bunick, C., Bam, J. and Bunick, D. 2001. Estrogen regulation of ion transporter messenger RNA levels in mouse efferent ductules are mediated differentially through estrogen receptor (ER) ${\alpha}$. and ER${\beta}$. Biol. Reprod. 65:1534-1541 https://doi.org/10.1095/biolreprod65.5.1534
  27. Hess, R., Bunick, D., Lee, K. -H., Barh, J., Taylor, J. A., Korach, K. S. and Lubahn, D. B. 1997. A role for oestrogens in the male reproductive system. Nature. 390:509-512 https://doi.org/10.1038/37352
  28. Bonen, A., Heynen, M. and Harta, H. 2006. Districution of monocarboxylate transporters MCT1-MCT8 in rat tissues and humans skeletal muscle. Appl. Physiol. Nutr. Metab. 31:31-39 https://doi.org/10.1139/H05-002