Evaluation of Angiotensin -I- Converting Enzyme Inhibitory Activity and Protein Changes of Enzymatic Hydrolysate Extracted from Hanwoo Loin and Round Myosin B

한우 등심과 우둔에서 추출한 Myosin B의 효소적 가수분해물의 단백질 변화와 Angiotensin -I- Converting Enzyme(ACE) 저해효과

  • Kim, Y.J. (Dept. of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University) ;
  • Chin, Koo-Bok (Dept. of Animal Science and Institute of Agricultural Science and Technology, Chonnam National University)
  • 김영주 (전남대학교 동물자원학부 식육과학 연구실 및 농업과학기술연구소) ;
  • 진구복 (전남대학교 동물자원학부 식육과학 연구실 및 농업과학기술연구소)
  • Published : 2007.02.28


This study was performed to determine the protein profiles using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Angiotensin-I-converting enzyme(ACE) inhibitory activity (IC50) as affected by the various meat cuts, digestion times with pepsin. Hydrolysates having the protein concentration of 10 ug/mL had approximately 36∼39% ACE inhibitory activities, regardless of meat cut and digestion time. Protein concentration and ACE inhibitory activity of the diluted hydrolysate increased after 1-hr digestion. In original hydrolysates, ACE inhibitory activities of loin had higher than those of round (P<0.05). In addition, non-heated hydrolysates had higher ACE inhibitory activities than heated counterparts. When myosin B was digested by pepsin more than 1 hr, improved ACE inhibitory activities were observed as compared to the non-digested control.


Angiotensin converting enzyme(ACE) inhibitory activity;Myosin B;Hanwoo loin and round


  1. SPSS. 1995. SPSS for Windows, 6.1.2. SPSS Inc. Chicago, IL. USA
  2. Fujita, H., Yokoyama, K. and Yoshikawa, M. 2000. Classification and antihyper- tensive activity of angiotensinI-converting enzyme inhibitory peptides derived from food proteins. J. Food Sci. 65(4): 564-569
  3. Gavras, H., Faxon, D. P., Berkoben, J., Brunner, H. R. and Ryan, T. J. 1978. Angiotensin converting enzyme inhibition in patients with congestive heart failure. Circulation. 58:770-776
  4. Jang, A. and Lee, M. 2005. Purification and identification of angiotensin converting enzyme inhibitory peptides from beef hydrolysates. Meat Sci. 69:653-661
  5. Jang, S. H., Jang, A., Kim, K. J., Cheon, Y. H., Min, J. S., Lee, S. O. and Lee, M. 2003. Purification and isolation for antihypertensive peptides from beef heart and spleen. Korean J. Food Sci. Technol. 45(2):319-326
  6. Je, J. Y., Park, J. Y., Jung, W. K., Park, P. J. and Kim, S. K. 2005. Isolation of angiotensin I converting enzyme (ACE) inhibitor from fermented oyster sauce, Crassostrea gigas. Food Chem. 90: 809-814
  7. Katayama, K., Fuchu, H., Sakata, A., Kawahara, S., Yamauch, K., Kawamura, Y. and Muguruma, M. 2003. Angiotensin I-converting enzyme inhibitory activities of porcine skeletal muscle proteins following enzyme digestion. Asian-Aust. J. Anim. Sci. 16(3):417-424
  8. Kim, S. Y., Kim, S. H. and Song, K. B. 2003. Purification of an ACE inhibitory peptide from hydrolysates of duck meat protein. Nutraceuticals & Food. 8:66-69
  9. Laemmli, U. K. 1970. Cleavage of structural pectins during the assembly of the head of the bacteriophage T4. Nature. 227:680-685
  10. Lowry, O. H., Roserbrough, N. J., Farr, A. L. and Randall, R. J. 1951. Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275
  11. Pihlanto-Leppala, A., Rokka, T. and Korhonen, H. 1998. Angiotensin-I-converting Enzyme Inhibitory peptides derived from bovine milk proteins. Int. Dairy J. 8:325-331
  12. Wong, J., Patel, R. A. and Kowey, P. R. 2004. The clinical use of angiotensin- converting enzyme inhibitors. Prog. Cardiovasc. Dis. 47(2):116-130
  13. Arihara, K., Nakashima, Y., Mukai, T., Ishikawa, S. and Itoh, M. 2001. Peptide inhibitors for angiotensin I-converting enzyme from enzymatic hydrolysates of porcine skeletal muscle proteins. Meat Sci. 57:319-324
  14. Athukorala, Y. and Jeon, Y. J. 2005. Screening for angiotensin-I-converting enzyme inhibitory activity of Ecklonia cava. J. Food Sci. Nutr. 10:134-139
  15. Coates, D. 2003. The angiotensin converting enzyme (ACE). Int. J. Biochem. Cell Biol. 35:769-773
  16. Cushman, D. W. and Cheung, H. S. 1971. Spectrophotometric assay and properties of the angiotensin-converting enzyme of rabbit lung. Biochem. Pharmacol. 20:1637-1648
  17. Fujita, H. and Yoshikawa, M. 1999. LKPNM : a prodrug-type ACE- inhibitory peptide derived from fish protein. Immunophamacol. 44:123-127