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Effects of Sex Steroid Hormones on Differentiation of Pig Preadipocytes

스테로이드 성호르몬이 돼지 지방전구세포의 분화에 미치는 영향

  • Kim, H.R. (Department of Animal Science, Chungbuk National University) ;
  • Lee, K.H. (Department of Biochemistry and Molecular Biology and Center of Antiaging, Eulji University) ;
  • Choi, I.H. (School of Biotechnology, Yeungnam University) ;
  • Chung, C.S. (Department of Animal Science, Chungbuk National University)
  • Published : 2007.10.31

Abstract

The current study was undertaken to determine the effects of sex steroid hormones(estrogen, testosterone and 19-nortestosterone) on differentiation and proliferation of pig preadipocytes. The preadipocytes were isolated from the backfat of new-born female pigs by collagenase digestion. 10-8M and 10-7M sex steroid hormones were treated to the cultured preadipocytes. Sex steroid hormones treated during the early stage of cell growth did not affect differentiation and proliferation of preadipocytes. However, testosterone and 19- nortestosterone treated during the late stage of cell growth stimulated differentiation of pig preadipocytes.

Keywords

Sex steroid;Pig;Preadipocytes;Differentiation

References

  1. Brandebourg, T. D. and Hu, C. Y. 2005. Isomerspecific regulation of differentiating pig preadipocytes by conjugated linoleic acids. J. Anim. Sci. 83:2096-2105 https://doi.org/10.2527/2005.8392096x
  2. De Wasch, K., Le Bizec, B., De Brabander, H., Andre, F. and Impens, S. 2001. Consequence of boar edible tissue consumption on urinary profiles of nandrolone metabolites. II. Identification and quantification of 19-norsteroids responsible for 19-nornandrosterone and 19-norretiocholanolone excretion in human urine. Rapid Commun. Mass spectrom. 15:1442-1447 https://doi.org/10.1002/rcm.391
  3. Dieudonne, M. N., Pecquery, R., Leneveu, M. C. and Giudicelli, Y. 2000. Opposite effects of androgens and estrogens on adipogenesis in rat preadipocytes: Evidence for sex and site-related specificities and possible involvement of insulin-like growth factor 1 receptor and peroxisome proliferator-activated receptor ${\gamma}2$. Endo. 141(2):649-656 https://doi.org/10.1210/en.141.2.649
  4. Garcia, E., Lacasa, M., Agli, B., Giudicelli, Y. and Lacasa, D. 1999. Modulation of rat preadipocyte adipose conversion by androgenic status: involvement of C/EBPs transcription factors. J. Endo. 161:89-97 https://doi.org/10.1677/joe.0.1610089
  5. Hauner, H., Schmid, P. and Pfeiffer, E. F. 1987. Glucocorticoid and insulin promote the differentiation of human adipocyte precusor cells into fat cells. J Clin Endocrinol Metab. 64:832-835 https://doi.org/10.1210/jcem-64-4-832
  6. Loffler, G. and Hauner, N. 1987. Adipose tissue development: the role precursor cells and adipogenic factors. Klin Wochenschr. 1:65-69
  7. Lu, C. C., Tasi, S. C., Wang, S. W., Tasi, C. L., Lau, C. P., Shih, H. C., Chen, Y. H., Chiao, Y. C., Liaw, C. and Wang, P. S. 1998. Effects of ovarian steroid hormones and thyroxine on calcitonin secretion in pregnant rats. Am. J. Physiol. 274:E246-252
  8. Roncari, D. A. K. and Van, R. L. R. 1978. promotion of human adipocyte precursor replication by 17-beta estradiol in culture. J Clin Invest. 62:503-508 https://doi.org/10.1172/JCI109153
  9. SAS. 1998. SAS Use's Guide: Statistics, SAS Inst, Int., Cary, N.C.
  10. Schwarzenberger, F., Toole, G. S., Christie, H. L. and Raeside, J. I. 1993. Plasma levels of several androgens and estrogens from birth to puburty in male domestic pigs. Acta. Endo. 128(2):173-177
  11. Singh, R., Artaza, J. N., Taylor, W. E., Braga, M., Yuan, X., Gonzalez-cadavid, N. F. and Bhasin, S. 2006. Testosterone inhibits adipogenic differentiation in 3T3-L1 cells: Nuclear translocation of androgen receptor complex with ${\beta}$-catanin and T-cell factor 4 May bypass canonical Wnt signaling to down-regulate adipogenic transcription factors. Endo. 147(1):141-154 https://doi.org/10.1210/en.2004-1649
  12. Singh, R., Artaza, J. N., Taylor, W. E., Gonzalezcadavid, N. F. and Bhasin, S. 2003. Androgens stimulates myogenic differentiation and inhibit adipogenesis in C3H 10T1/2 pluripotent cells through an androgen receptor-mediated pathway. J. Endo. Soci. 144(11):5081-5088
  13. Snyder, P. J., Peachey, H., Berlin, J. A., Hannoush. P., Haggad, G., Dlewati, A., Santanna, J., Loh, L., Lenrow, D. A., Holmes, J. H., Kappor, S., Atkinson, L. and Strom, S. 2000. Effects of testosterone replacement in hypogonadal men. J. Clin. Endo. Metab. 85:2670-2677 https://doi.org/10.1210/jc.85.8.2670
  14. Suryawan, A., Swanson, L. V. and Hu, C. Y. 1997. Insulin and hydrocortisone, but not triiodothyronine, and required for the differentiation of pig preadipocytes in primary culture. J. Anim. Sci. 75:105-111
  15. Vanderschueren, D., Vandenput, L., Boonen, S., Van Herck, E., Swinnen, J. V. and Bouillon, R. 2000. An aged rat model of partial androgen deficiency: prevention of both loss of bone and lean body mass by low-dose androgen replacement. Endo. 141:1642-1647 https://doi.org/10.1210/en.141.5.1642
  16. Wise, L. S. and Green, H. 1979. participation of one isozyme of cytosolic glycerophosphate in adipose conversion of 3T3 cells. J. Biol. Chem. 254:273-275
  17. 문현석, 정정수. 2004. Conjugated linoleic acid (CLA) 이성체가 돼지 지방전구세포의 분화에 미치는 영향. 한국동물자원과학회지 46(6):967-974
  18. Hong, L., Colpan, A., Peptan, I. A., Daw, J., George, A. and Evans, C. A. 2007. 17-Beta estradiol enhances osteogenic and adipogenic differentiation of human adipose-derived stromal cells. Tissue. Eng. 13(6):1197-203 https://doi.org/10.1089/ten.2006.0317