Screening of Chicken Genes Related to Germ Cell Development

닭에서 생식세포 발달에 관여하는 유전자 검색

  • Lee, Jee-Young (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Hee-Bal (School of Agricultural Biotechnology, Seoul National University) ;
  • Kim, Duk-Kyung (School of Agricultural Biotechnology, Seoul National University) ;
  • Song, Ki-Duk (Biotechnology Institute, Hanlim Human Tower) ;
  • Lim, Jeong-Mook (School of Agricultural Biotechnology, Seoul National University) ;
  • Han, Jae-Yong (School of Agricultural Biotechnology, Seoul National University)
  • 이지영 (서울대학교 농업생명과학대학) ;
  • 김희발 (서울대학교 농업생명과학대학) ;
  • 김덕경 (서울대학교 농업생명과학대학) ;
  • 송기덕 (아비코아 생명공학연구소) ;
  • 임정묵 (서울대학교 농업생명과학대학) ;
  • 한재용 (서울대학교 농업생명과학대학)
  • Published : 2007.04.30


We examined the expression patterns of the chicken TCs(tentative consensus sequences) originated from GermOnline genes in various chicken tissues, applying information from GermOnline to chicken organisms. 42 TCs among 84 chicken homologous TCs from the pool of 84 genes related to germ cell lineage in mouse(10), rat(71) and human(3) had high homology based on a BLAST search. Of these, Hmgcs2 and Sycp3 was shown to be expressed in a testis- specific manner and a reproductive organ(testis and ovary)-specific manner, respectively, by RT- PCR analysis. Crmp4, Cyct, Ldhc, Epha7, Pcsk4 and Dnmt3a are expressed in brain, testis, and ovary. The characterization of chicken genes originated from GermOnline in this research may give an enormously useful source of information related to germ cell development.


Chicken;Germ cell;GermOnline;Hmgcs2;Sycp3


  1. Gil Gomez, G., Ayte, J. and Hegardt, F. G. 1993. The rat mitochondrial 3-hydroxy-3-methyl- glutaryl-coenzyme-A-synthase gene contains elements that mediate its multihormonal regulation and tissue specificity. Eur. J. Biochem. 213(2):773-9
  2. Hata, K., Okano, M., Lei, H. and Li, E. 2002. Dnmt3L cooperates with the Dnmt3 family of de novo DNA methyltransferases to establish maternal imprints in mice. Development. 129(8):1983-93
  3. Healy, C., Uwanogho, D. and Sharpe, P. T. 1996. Expression of the chicken Sox9 gene marks the onset of cartilage differentiation. Ann. N. Y. Acad. Sci. 785:261-2
  4. Irie, M., Hata, Y., Deguchi, M., Ide, N., Hirao, K., Yao, I., Nishioka, H. and Takai, Y. 1999. Isolation and characterization of mammalian homologues of Caenorhabditis elegans lin-7: localization at cell-cell junctions. Oncogene. 18(18):2811-7
  5. Kent, J., Wheatley, S. C., Andrews, J. E., Sinclair, A. H. and Koopman, P. 1996. A male- specific role for SOX9 in vertebrate sex deter- mination. Development. 122(9):2813-22
  6. Lockhart, D. J. and Winzeler, E. A. 2000. Genomics, gene expression and DNA arrays. Nature. 405:827-836
  7. Nakayama, K., Kim, W. S., Torii, S., Hosaka, M., Nakagawa, T., Ikemizu, J., Baba, T. and Murakami, K. 1992. Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis- specific expression. J. Biol. Chem. 267(9):5897- 900
  8. Reik, W. and Walter, J. 2001. Evolution of imprinting mechanisms: the battle of the sexes begins in the zygote. Nature Genetics. 27:255-256
  9. Sakai, I., Sharief, F. S. and Li, S. S. L. 1987. Molecular cloning and nucleotide sequence of the cDNA for sperm-specific lactate dehydrogenase-C from mouse. Biochem. J. 242:619-622
  10. Schlecht, U., Demougin, P., Koch, R., Hermida, L., Wiederkehr, C., Descombes, P., Pineau, C., Jegou, B. and Primig, M. 2004. Expression profiling of mammalian male meiosis and gameto- genesis identifies novel candidate gene for roles in the regulation of fertility. Mol. Biol. Cell. 15(3): 1031-43
  11. Schlecht, U. and Primig, M. 2003. Mining meiosis and gametogenesis with DNA microarrays. Reproduction. 125:447-56
  12. Shima, J. E., McLean, D. J., McCarrey, J. R. and Griswold, M. D. 2004. The murine testicular transcriptome: characterizing gene expression in the testis during the progression of spermato- genesis. Biol. Reprod. 71(1):319-30
  13. Valenzuela, D. M., Rojas, E., Griffiths, J. A., Compton, D. L., Gisser, M., Ip, N. Y., Goldfarb, M. and Yancopoulos, G. D. 1995. Identification of full-length and truncated forms of Ehk-3, a novel member of the Eph receptor tyrosine kinase family. Oncogene. 10(8):1573-80
  14. Wang, L. H. and Strittmatter, S. M. 1996. A family of rat CRMP genes is differentially expressed in the nervous system. J. Neurosci. 16(19):6197-207
  15. Wiederkehr, C., Basavaraj, R., Sarrauste, de Menthiere, C., Koch, R., Schlecht, U., Hermida, L., Masdoua, B., Ishii, R., Cassen, V., Yamamoto, M., Lane, C., Cherry, M., Lamb, N. and Primig, M. 2004a. Database model and specification of GermOnline Release 2.0, a cross-species community annotation knowledgebase on germ cell differenti- ation. BIOINFORMATICS. 20(5):808-811
  16. Wiederkehr, C., Basavaraj, R., Sarrauste, de Menthiere, C., Hermida, L., Koch, R., Schlecht, U., Amon, A., Brachat, S., Breitenbach, M., Briza, P., Caburet, S., Cherry, M., Davis, R., Deutschbauer, A., Dickinson, H. G., Dumitrescu, T., Fellous, M., Goldman, A., Grootegoed, J. A., Hawley, R., Ishii, R., Jegou, B., Kaufman, R. J., Klein, F., Lamb, N., Maro, B., Nasmyth, K., Nicolas, A., Orr-Weaver, T., Philippsen, P., Pineau, C., Rabitsch, K. P., Reinke, V., Roest, H., Saunders, W., Schroder, M., Schedl, T., Siep, M., Villeneuve, A., Wolgemuth, D. J., Yamamoto, M., Zickler, D., Esposito, R. E. and Primig, M. 2004b. GermOnline, a cross-species community knowledgebase on germ cell differentiation. Nucleic Acids Research. 32:560-7
  17. Lammers, J. H., Offenberg, H. H., van Aalderen, M., Vink, A. C., Dietrich, A. J. and Heyting, C. 1994. The gene encoding a major component of the lateral elements of synaptonemal complexes of the rat is related to X-linked lymphocyte- regulated genes. Mol. Cell. Biol. 14(2):1137-46
  18. Miyamoto, T., Hasuike, S., Yogev, L., Maduro, M. R., Ishikawa, M., Westphal, H. and Lamb, D. J. 2003. Azoospermia in patients heterozygous for a mutation in SYCP3. Lancet. 362(9397):1714-9
  19. Seidah, N. G., Day, R., Hamelin, J., Gaspar, A., Collard, M. W. and Chretien, M. 1992. Testicular expression of PC4 in the rat: molecular diversity of a novel germ cell-specific Kex2/subtilisin-like proprotein convertase. Mol. Endocrinol. 6(10): 1559-70