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Effect of Glycerol Concentration, Freezing Rate and Thawing Rate on Semen Characteristics in PoongSan-dog

풍산개 정자의 동결보존에 있어서 Glycerol 농도, 동결 및 융해속도가 정자성상에 미치는 영향

  • Ji, D.Y. (Seoul Grand Park Zoo) ;
  • Yoon, T.C. (Korea University Institute of life Science and Natural Resources) ;
  • Rho, J.R. (Seoul Grand Park Zoo) ;
  • Cho, S.R. (National Institute of Animal Science, RDA) ;
  • Kim, C.K. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Pang, M.G. (Department of Animal Science & Technology, Chung-Ang University) ;
  • Kim, Bo-Sook (Seoul Grand Park Zoo)
  • Published : 2007.10.31

Abstract

This research was carried out in order to establish the production technique for Poong-san dog’s frozen semen, by examining the semen characteristic and the volume of glycerol added to the dilution solution, thawing temperature and sperm motility and viability as well as the motility using CASA according to time variation. Average semen volume was 5.9ml, sperm concentration 116.3×106 sperm/ml, total sperm number 789.3×106 sperm, motility 88.7±1.7% and viability 87.6±7.8%. When it was cryopreservation and thawed at different glycerol concentrated extender, it showed 52.7% motility and 57.7±10.3% viability at 7% glycerol, compared to other treatments. For semen cryogeny, at conditions of 5, 7cm and a height of 10cm for pre-cryogeny and maintaining the semen at 7cm from the surface of liquid nitrogen resulted in profitable motility and viability.

Keywords

PoongSan-dog;Semen characteristic;Glycerol;Freezing;Thawing

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