Diurnal Patterns in the Flow of Escapable Soluble Non-Ammonia Nitrogen Fractions in Omasal Digesta as Influenced by Barley and Rapeseed Meal Supplementation in Cows Fed Grass Silage Based Diet

목초 사일리지 급여 시 보리와 채종박 보충급여에 의한 제 3위 소화액내 Soluble Non-ammonia Nitrogen Fraction의 Flow 패턴 변화

  • Choi, C.W. (National Institute of Animal Science, RDA)
  • 최창원 (농촌진흥청 축산과학원)
  • Published : 2007.06.30


The present study was conducted to measure diurnal patterns in the flow of soluble non-ammonia nitrogen (SNAN) fractions in the liquid phase of digesta entering the omasum of cows fed grass-red clover silage supplemented with barley and rapeseed meal. Four ruminally cannulated cows were fed, in a 4×4 Latin square design, grass-red clover silage alone (GS) or supplemented with (on a DM basis) 6.0kg/d of barley grain, 2.1kg/d of rapeseed meal or 6.0kg/d of barley and 2.1kg/d rapeseed meal. Omasal digesta was taken using an omasal sampling system at 1.5h intervals during a 12h feeding cycle, and SNAN fractions (free AA, peptide and soluble protein) in the omasal digesta were assessed using ninhydrin assay. Dietary supplementation numerically increased the mean flow of SNAN fractions relative to GS diet despite the lack of statistical significance. Diurnal patterns in the flow of peptide entering the omasum during a 12h feeding cycle appeared to be highest immediately after feeding, declined by 10.0h post-feeding and slightly increased thereafter. In SNAN fractions, the flow of peptide was higher for supplemented diets than for GS diet throughout the feeding cycle. Based on the microbial contribution to total SNAN using 15N, diurnal patterns in the flow of dietary SNAN for dietary supplemented diets appeared to be higher compared with GS diets. Present results may conclude that peptide flow is quantitatively the most important N in SNAN fractions and that dietary supplementation can increase peptide flow entering the omasal canal.


Supplementation;Soluble Non-Ammonia Nitrogen;Omasal Digesta


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