Conjugated Linoleic Acid(CLA)가 돼지 지방세포와 근육세포의 증식과 분화에 미치는 영향

Effect of Conjugated Linoleic Acid(CLA) on Proliferation and Differentiation of Porcine Adipocyte and Muscle Cell

  • 정정수 (충북대학교 농업생명환경대학 축산학과) ;
  • 김혜림 (충북대학교 농업생명환경대학 축산학과) ;
  • 강지나 (충북대학교 농업생명환경대학 축산학과) ;
  • 김내수 (충북대학교 농업생명환경대학 축산학과)
  • Chung, C.S. (Department of Animal Science, Chungbuk National University) ;
  • Kim, H.R. (Department of Animal Science, Chungbuk National University) ;
  • Kang, J.N. (Department of Animal Science, Chungbuk National University) ;
  • Kim, N.S. (Department of Animal Science, Chungbuk National University)
  • 발행 : 2007.02.28


본 연구는 여러 CLA 이성체, 즉 cis-9, cis- 11(c9c11), cis-9, trans-11(c9t11), trans-9, trans-11 (t9t11) 및 trans-10, cis-12(t10c12)가 배양 중인 돼지 지방세포와 근육세포의 분화와 증식에 미치는 영향을 구명하고자 실시하였다. 세포는 신생자돈으로부터 분리했다. t10c12 이성체는 지방세포의 분화를 억제했는데(92%) 근육세포의 분화는 억제시키지 않았다. t9t11 이성체는 지방세포의 분화를 억제했는데(14%), 근육세포의 분화는 촉진시켰다(26%). 다른 CLA 이성체는 지방세포나 근육세포의 분화에 영향을 미치지 않았다. 그리고 CLA가 지방세포와 근육세포의 증식에 미치는 영향은 분화에 미치는 영향에 비해서 작았다. 위의 결과는 여러 CLA 이성체는 돼지 지방세포와 근육세포의 분화에 다른 영향을 미침을 나타낸다.


CLA;Pig;Preadipocyte;Myogenic satellite cells;Differentiation


  1. Azain, M. J. 2004. Role of fatty acids in adipocyte growth and development. J. Anim. Sci. 82:916- 924
  2. Brandebourg, T. D. and Hu, C Y. 2005. Isomerspecific regulation of differentiating pig preadipocytes by conjugated linoleic acids. J. Anim, Sci. 83:2096-2105
  3. Brown, J. M. and McIntosh, M. K. 2003. Conjugated linoleic acid in humans: Regulation of adiposity and insulin sensitivity. J. Nutr. 133: 3041-3046
  4. Doumit, M. E. and Merkel, R A. 1992. Conditions for isolation and culture of porcine myogenic satellite cells. Tissue and cell. 24:253-262
  5. Dugan, M. E., Aalhus, J. L. and Kramer, J. K. 2004. Conjugated linoleic acid pork research. Am. J. Clin. Nutr. 79:1212S-1216S
  6. Dugan, M. E., Aalhus, J. L., Schaefer, A. L. and Kramer, J. K. G. 1997. The effect of conjugated linoleic acid on fat to lean repartitioning and feed conversion in pigs. Can. J. Anim. Sci. 77:723- 725
  7. Pariza, M. W., Park, Y. and Cook, M. E. 2000. Mechanisms of action of conjugated linoleic acid: Evidence and speculation. Proc. Soc. Exp. Biol, Med. 223:8-13
  8. Park, Y., Jayne, M. S., Karen, J. A., Wei, L. and Michael, W. P. 1999. Evidence that the trans-10, cis-12 isomer of conjugated linoleic acid induces body composition changes in mice. Lipids. 34: 235-241
  9. SAS. 1998. SAS user's guide. SAS Inst. Inc., Cary, NC, USA
  10. Suryawan, A., Swanson, L. V. and Hu, C. Y. 1997. Insulin and hydrocortisone, but triiodothyronine, are required for the differentiation of pig preadipocytes in primary culture. J. Anim. Sci. 75:105-111
  11. Hurley, M. S., Flux, C, Salter, A. M. and Brameld, J. M. 2006. Effects of fatty acids on skeletal muscle cell differentiation in vitro. Br. J. Nutr. 95:623-630
  12. Kang, K., Liu, W., Albright, K. J., Park, Y. and Pariza, M. W. 2003. Trans-10, cis-12 CLA inhibits differentiation of 3t3-11 adipocytes and decreases PPAR expression. Biochem. Biophys. Res. Commun. 303:795-799
  13. McNeel, R. L. and Mersmann, H. J. 2003. Effects of isomers of conjugated linoleic acid on porcine adipocyte growth and diffemtiation. J. Nutr. Biochern. 14:266-274
  14. 문현석, 정정수. 2004. Conjugated Linoleic Acid (CLA) 이성체가 돼지 지방전구세포의 분화에 미치는 영향. 동물자원지 46:967-974
  15. Evans, M., Park, Y., Pariza, M., Curtis, L., Kuebler, B. and McIntosh, M. 2001. Trans-In, cis-12 conjugated linoleic acid reduces triglyceride content, while differentially affecting peroxisome proliferator activated receptor gamma2 and AP2 expression in 3t3-11 preadipocytes. lipids 36:1223-1232
  16. Brodie, A. E., Manning, V. A., Ferguson, K. R, Jewell. D. E. and Hu, C Y. 1999. Conjugated linoleic acid inhibits differentiation of pre- and post- confluent 3T3-L1 preadipocytes but inhibits cell proliferation only in preconfluent cells. J. Nutr. 129:602-606
  17. Brown, J. M., Halvorsen, Y. D., Lea Curris, Y. R, Geigerman, C. and McIntosh, M. 2001. Trans-10, cis-12, but not cis-9, trans-11, conjugated linoleic aicd attenuates lipogenesis in primary cultures of stromal vascular cells from human adipose tissue. J. Nutr. 131:2316-2321
  18. Wang, Y. W. and Jones, P. J. 2004. Conjugated linoleic acid and obesity control: effecacy and mechanisms. Int. J. Obes. Relat. Metab. Disord. 28:941-955
  19. Ostrowska, E., Suster, D., Muralitharan, M., Cross, R. F., Leury, B. J., Bauman, D. E. and Dunshea. F. R. 2003. Conjugated linoleic aicd decrease fat accretion in pigs: evalution by dual-energy X-ray absorptiometry. J. Nutri. 89:219-229