A Molecular Sex Identification Using Duplex PCR Method for SRY and ZFX-ZFY Genes in Red Deer and Elk

붉은사슴과 엘크에서 SRY와 ZFX-ZFY 유전자의 Duplex PCR기법을 이용한 성 판별

  • Han, S.H. (National Institute of Subtropical Agriculture, Rural Development Administration) ;
  • Lee, S.S. (National Institute of Subtropical Agriculture, Rural Development Administration) ;
  • Ko, M.S. (National Institute of Subtropical Agriculture, Rural Development Administration) ;
  • Cho, I.C. (National Institute of Subtropical Agriculture, Rural Development Administration)
  • 한상현 (농촌진흥청 난지농업연구소) ;
  • 이성수 (농촌진흥청 난지농업연구소) ;
  • 고문석 (농촌진흥청 난지농업연구소) ;
  • 조인철 (농촌진흥청 난지농업연구소)
  • Published : 2007.02.28


This study was focused on discriminating the molecular sexes of red deer and elk by duplex polymerase chain reaction(PCR) using two primer sets. Sex differentiation of mammals is primarily dependent on the presence or absence of sex determining region Y(SRY) gene encoded on Y chromosome which plays a key role for male development. Zinc finger X-Y(ZFX-ZFY) gene, one of X-Y homology gene group was found on X- and Y- chromosomes, respectively. At first, the nucleotide sequences were characterized for the intron 9 flanking region of ZFX-ZFY genes. The intron 9 of ZFX and ZFY is 529-bp and 665-bp in length, respectively. A transposable element sequence similar to bovine SINE element Bov-tA was detected only in ZFY gene of Cervidae. Sexing analysis was conducted by duplex PCR assay for amplification of SRY and ZFX-ZFY genes. Two differentially amplified patterns were found: one for females has a common band amplified only from ZFX as a template, and another for males had three bands(a common ZFX and two male-specific ZFY and SRY). On the separate tests using each gene, the results was identical to those from duplex PCR assay. Moreover, the results from PCR assays provide also identical information to phenotypic investigation of individuals of red deer, elk as well as their hybridized progenies collected from two isolated farms. These results suggest that it may be a rapid and precise method for determining the sexes by duplex PCR amplification using Y-chromosome specific SRY and X- and Y- homologous ZFX-ZFY genes showing sexual dimorphism in red deer and elk without any other controls.


Elk;Molecular sexing;Red deer;SRY;ZFX-ZFY


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