Monitoring of Feed-Nutritional Components, Toxic Heavy Metals and Pesticide Residues in Mushroom Substrates According to Bottle Type and Vinyl Bag Type Cultivation

버섯의 봉지재배 및 병재배 시 재배단계별 배지의 사료영양적 성분, 독성중금속 및 잔류농약 모니터링

  • Kim, Y.I. (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University) ;
  • Bae, J.S. (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University) ;
  • Huh, J.W. (Kyonggi-do Institute of Health & Environment) ;
  • Kwak, W.S. (Animal Science, School of Life Resource and Environmental Sciences, College of Natural Sciences, Konkuk University)
  • 김영일 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 배지선 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공) ;
  • 허정원 (경기도 보건환경연구원) ;
  • 곽완섭 (건국대학교 자연과학대학 생명자원환경과학부 축산학전공)
  • Published : 2007.02.28


This study was carried out to monitor feed-nutritional components, toxic heavy metals (Cd, Pb and As) and pesticide residues through three cultivation stages (1st initial culture stage, 2nd mycelial growth stage, and 3rd fruit body-harvested stage) of king oyster mushroom (Pleurotus eryngii) produced by bottle type cultivation and oyster mushroom (Pleurotus osteratus) produced by vinyl bag type cultivation. For both cultivation types, compared with the initial culture, the weight reduction rate in spent mushroom substrates (SMS) after fruit body harvest was 29% for total wet mass, 21~25% for dry and organic matters and 19 ~22% for neutral detergent fiber. Two thirds to 3/4 of organic matter degraded and utilized by mycelia and fruit bodies was originated from fiber, of which the primary source (50~70%) was hemicellulose. The effect of mycelial growth stage on chemical compositional change in culture was little (P>0.05) for bottle type cultivation of king oyster mushroom but considerable (P<0.05) for vinyl type cultivation of oyster mushroom. Culture nutrients uptake by fruit bodies was very active for the bottle type cultivation. Compared with SMS, harvested fruit bodies (mushrooms) contained higher (P<0.05) crude protein, non-fibrous carbohydrate, and crude ash and lower (P<0.05) neutral detergent fiber. Regardless of stages, no culture samples were contaminated with toxic heavy metals and pesticide residues. In conclusion, the increase of fiber (neutral and acid detergent fibers) and indigestible protein contents and the decrease of true protein content in SMS indicated that the feed-nutritional value of SMS was significantly reduced compared with that of the initial culture and they were safe from toxic heavy metals and pesticide residues.


Spent mushroom substrate;Spent mushroom compost;Mushroom;Byproduct;Recycling;Feed


  1. 김영일, 배지선, 정세형, 안문환, 곽완섭. 2007. 버섯폐배지의 발생량 조사 및 새송이, 느타리, 팽이 버섯 폐배지의 버섯종류별과 재배방식별의 물리화학적 특성 평가. 한국동물자원과학회지 49(1):913-922
  2. 김용국, 김용인. 1993. Mycelium에 의한 Lignocellulose 물질로부터 양질의 발효사료 생산. 한국낙농학회지. 15(4):251-260
  3. 농림부. 2001. 사료 내 위해물질과 잔류농약 범위 및 허용치. 농림부 고시 2001-61, 농림부, 대한민국
  4. 배지선. 2006. 버섯 폐배지의 반추동물 조사료원으로서의 사료 영양적 가치에 대한 기초 평가. 건국대학교 석사학위 논문
  5. 식약청. 2001. 식품공전(별책). 132-133, 258-263
  6. 윤승락. 1996. 표고버섯 재배 톱밥 폐배지의 가축 조사료 이용. 월간 축산인. 2월호 :124
  7. 홍무기, 원경풍, 황인균, 최동미, 이강봉, 오금순, 허수정. 2002. 식품중 잔류농약 모니터링. 식품의약품안전청연구보고서 제6권 The Annual Report of KFDA, Vol(6):67-75
  8. Rajathan, S. and Bano, Z., 1989. Pleurotus mushrooms. Biotransformations of natural lignocellulotic waste. Critical Rev. Food Sci. Nutr., Vol. 28. Mysore, India, pp. 31-123
  9. Semple, K. T., Watts, N. U. and Fermor, T. R. 1998. Factors affecting the mineralization of (U14C) benzene in spent mushroom substrate. FEMS Microbiology Letters 164(2):317-321
  10. Semple, K. T. and Fermor, T. R. 1995. The bioremediation of enobiotic-contamination by composts and associated microflora. Mushroom Science 14(2): 917-924
  11. Staments, P. 2001. Mycova : Helping the ecosystem through mushroom cultivation. bioremediation/index.html (June 29, 2001)
  12. Tuomela, M., Vikman, M., Hatakka, A. and Itavaara, M. 2000. Biodegradation of lignin in a compost enviroment:a review. Bioresource Tech 72:169-183
  13. Mark, W. P., Hadar, Y. and Dan, C. 1984. Fungal activities involved in lignocellulose degradation by pleurotus. Appl Microbial Biotechnol 20:150-154
  14. Martirani, L., Giardina, P., Marzullo, L. and Sannia, G. 1996. Reduction of phenol content and toxicity in olive oil mill waste waters with the ligniolytic fungus Pleurotus ostreatus. Water Research 30(8): 1914-1918
  15. Mashphy, S., Levanon, D. and Henis, Y. 1996. Degradation of atrazine by the lignocellulolytic fungus pleurotus pulmonarius during solid-state fermentation. Bioresource Tech 56:207-214
  16. National Research Council. 1980. Mineral Tolerance of Domestic Animals. National Academy Press, Washington, DC, USA
  17. Adamovic, M., Grubi, G., Milenkovic, I., Jovanovi, R., Proti, R., Sretenovi, L. and Stoievi, L. 1998. The biodegradation of wheat straw by Pleurotus ostreatus mushrooms and its use in cattle feeding. Animal Feed Science Technology 71:357-362
  18. Andrew, S. B. and Anita, M. J. 1995. The recovery of lignocellulose-degrading enzymes from spent mushroom compost. Bioresoruce Tech. 54:311-314
  19. Bakshi, M. P. S., Gupta, V. K. and Langar, P. N. 1985. Acceptability and nutritive evaluation of Pleurotus harvested spent wheat straw in buffaloes. Agricultural Wastes 13:51-58
  20. Baskaran, S., Bolan, N. S., Rahmanm, A and Tillman, R. W. 1996. Effect of exogenous carbon on the sorption and movement of atrazine and 2,4-D by soils. Australian Journal of Soil Research 34(4):609-622
  21. Calzada, J. F., Franco, L. F., Arriola, M. C., Rolz, C. and Ortiz, M. A. 1987. Acceptability, body weight changes and digestibility of spent wheat straw after harvesting of Pleurotus sajor-caju. Biological Wastes 22:303-309
  22. CODEX. 2006. Draft And proposed draft maximum residue limites in foods and feeds at seep 7 and 4, including dried chili peppers at step 7
  23. Fermor, T., Watts, N., Duncombe, T., Brooks, R., McCarthy, A., Semple, K. and Reid, B. 2000. Bioremediation: use of composts and composting echnologies. Mushroom Science 15:833-839
  24. Hadar, Y., Zohar, K. and Barbara, G. 1993. Biodegradation of lignocellulosic agrocultural wastes by Pleurotus ostreatus. Journal of Biotechnology 30:133-139
  25. Kuo, W. S. and Regan, R. W. 1998. Aerobic carbamate bioremediation aided by compost residuals from the mushroom industry: Laboratory Studies. Compost Science and Utilization 6(1):19-29
  26. Makela, M., Galkin, S., Hatakka, A. and Lundell, T. 2002. Production of organic acids and oxalate decarboxylase in lignin-degrading white rot fungi. Enzyme and Microbial Technology 30:542-549
  27. Manuel, V., Gonzalo, A. and Angel, T. M. 1990. Substrate-dependent degradation patterns in the decay of wheat straw and veech wood by ligninolytic fungi. Appl Microbiol Biotechnol 33:481-484
  28. Ehlers, G. A. and Rose, P. D. Immobilized white- rot fungla biodegradation of phenol and chlorinated phenol in trickling packed-bed reactors by employing sequencing batch operation. 2004, Bioresource Tech 96:1264-1275
  29. Kannan, K. and Oblisami, G. 1990. Enzymology of ligno-cellulose degradation by pleurotus sajor-caju during geowth on paper-mill sludge. Biological wastes 33:1-8

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