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Survival Rate, Developmental Competence and Sex Ratio of Post-thawed Hanwoo Embryo Following Biopsy

한우 수정란의 Biopsy 후 배발달율과 동결-융해후 생존성 및 성비

  • Cho, S.R. (National Livestock Reserch Institute, RDA) ;
  • Choi, S.H. (National Livestock Reserch Institute, RDA) ;
  • Kim, H.J. (National Livestock Reserch Institute, RDA) ;
  • Choe, C.Y. (National Livestock Reserch Institute, RDA) ;
  • Jin, H.J. (National Livestock Reserch Institute, RDA) ;
  • Cho, C.Y. (National Livestock Reserch Institute, RDA) ;
  • Son, D.S. (National Livestock Reserch Institute, RDA)
  • Published : 2007.04.30

Abstract

The present study was to conducted the sexing efficiency and accuracy of bovine embryo by LAMP (Loop-mediated isothermal amplification) method, the development of the biopsied embryos into re- reformation and the freezability of these blastocysts by slow-freezing and vitrification. In vivo embryos were superovaluted with gonadotropin(Antorin R-10) for 4 days combined with progestrone releasing intravaginal(CIDR) insertion in Hanwoo donors, and in vitro embryos were used blastocyst embryos at Day 7 or Day 8 after post-insemination in vitro. The biopsy of bovine embryo was carried out in a 80μl drop with Ca2+-Mg2+ free D-PBS and the viability of biopsied embryos were evaluated in IVMD medium at over 12 h culture time in 5% CO2 incubator.For embryo sexing, about five or seven blastomeres were isolated from in vitro and in vivo embryos at blastocysts with microblade. and were then subjected to LAMP. The survivability of biopsied embryos were no difference in the development rate to re-formation of blastocysts between in vivo and in vitro embryos(100% and 90% respectively). The rates of sexed embryos were compared according to two groups, the female rate was lower than that the male in the in vivo and in vitro embryos(46% vs, 54% and 40% vs, 60%, respectively). However, there were no difference in the overall sexing ratio between the two groups. The survivability of frozen-thawed sexed embryos were lower in the in vitro than in vivo embryos in the slow-freezing(Group 1) and vitrification method(Group 2), (41.7% vs. 58.8% and 57.1% vs, 77.8. respectively).

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