Expression of lewis antigen in gastric mucosa of children with Helicobacter pylori infection

Helicobacter pylori에 감염된 소아의 위점막에서 Lewis 항원의 발현

  • Chung, Ju-Young (Department of Pediatrics, Sanggyepaik Hospital, Inje University College of Medicine) ;
  • Lim, Seong Jig (Department of Pathology, Sanggyepaik Hospital, Inje University College of Medicine) ;
  • Han, Tae Hee (Department of Laboratory Medicine, Sanggyepaik Hospital, Inje University College of Medicine)
  • 정주영 (인제대학교 의과대학 소아과학교실) ;
  • 임성직 (인제대학교 의과대학 진단병리학교실) ;
  • 한태희 (인제대학교 의과대학 진단검사의학교실)
  • Published : 2007.05.30

Abstract

Purpose : Lewis antigen has been known to have a role in the attachment of H. pylori to the gastric mucosa, but its expression pattern in children with H. pylori infection is still unclear. The recently described blood group antigen-binding adhesin BabA is known to mediate adherence of H. pylori to Lewis B receptors on gastric epithelium. We investigated the expression of Lewis antigen in gastric mucosa of Korean children with H. pylori infection. Methods : The expression of Lewis A ($Le^a$), B ($Le^b$), X ($Le^x$), and Y ($Le^y$) was evaluated by immunohistochemistry in H. pylori positive biopsy specimens from 35 children (antral gastritis in 30, peptic ulcer in 5) and in H. pylori negative specimens from 19 children. PCR assays for cagA and babA2 gene of H. pylori were performed. Results : We confirmed the expression of $Le^a$ in 60%, $Le^b$ in 97%, $Le^x$ in 100%, and $Le^y$ in 100% of the superficial epithelium of the 35 H. pylori positive children. In H. pylori negative patients, $Le^a$, $Le^b$, $Le^x$, and $Le^y$ expression was 52%, 100%, 89%, and 100%, respectively. The cagA gene was detected in 65% and babA2 gene in 25% of 35 patients. No differences in neutrophil activity and chronic inflammation were found according to the presence of cagA and babA2 genes in H. pylori. Conclusion : $Le^b$, $Le^x$ and $Le^y$ antigen were highly expressed in gastric mucosa of Korean children, but they were not associated with the status of H. pylori infection and the positivity of babA2 gene. Further studies for other mucosal receptors and toxins are needed to define the immune responses to H. pylori infection in gastric mucosa of Korean children.