Expression of EuNOD-ARP1 Encoding Auxin-repressed Protein Homolog Is Upregulated by Auxin and Localized to the Fixation Zone in Root Nodules of Elaeagnus umbellata

  • Kim, Ho Bang (School of Biological Sciences, College of Natural Sciences, Seoul National University) ;
  • Lee, Hyoungseok (School of Biological Sciences, College of Natural Sciences, Seoul National University) ;
  • Oh, Chang Jae (School of Biological Sciences, College of Natural Sciences, Seoul National University) ;
  • Lee, Nam Houn (School of Biological Sciences, College of Natural Sciences, Seoul National University) ;
  • An, Chung Sun (School of Biological Sciences, College of Natural Sciences, Seoul National University)
  • Received : 2006.11.01
  • Accepted : 2006.12.12
  • Published : 2007.02.28

Abstract

Root nodule formation is controlled by plant hormones such as auxin. Auxin-repressed protein (ARP) genes have been identified in various plant species but their functions are not clear. We have isolated a full-length cDNA clone (EuNOD-ARP1) showing high sequence homology to previously identified ARP genes from root nodules of Elaeagnus umbellata. Genomic Southern hybridization showed that there are at least four ARP-related genes in the genome of E. umbellata. The cDNA clone encodes a polypeptide of 120 amino acid residues with no signal peptide or organelle-targeting signals, indicating that it is a cytosolic protein. Its cytosolic location was confirmed using Arabidopsis protoplasts expressing a EuNOD-ARP1:smGFP fusion protein. Northern hybridization showed that EuNOD-ARP1 expression was higher in root nodules than in leaves or uninoculated roots. Unlike the ARP genes of strawberry and black locust, which are negatively regulated by exogenous auxin, EuNOD-ARP1 expression is induced by auxin in leaf tissue of E. umbellata. In situ hybridization revealed that EuNOD-ARP1 is mainly expressed in the fixation zone of root nodules.

Keywords

Auxin-repressed Protein Gene;Cellular Localization;Elaeagnus umbellata;Frankia;In Situ Hybridization;Root Nodule

Acknowledgement

Supported by : Korean Ministry of Science and Technology

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