DOI QR코드

DOI QR Code

Biohydrogenation Pathways for Linoleic and Linolenic Acids by Orpinomyces Rumen Fungus

  • Nam, I.S. (National Institute of Animal Science, RDA) ;
  • Garnsworthy, P.C. (National Institute of Animal Science, RDA)
  • Received : 2007.02.14
  • Accepted : 2007.05.14
  • Published : 2007.11.01

Abstract

The objective of this study was to identify biohydrogenation pathways for linoleic, linolenic, oleic and stearic acids by Orpinomyces species of rumen fungus during in vitro culture. Biohydrogenation of linoleic acid produced conjugated linoleic acid (cis-9, trans-11 C18:2), which was then converted to vaccenic acid (trans-11 C18:1) as the end product of biohydrogenation. Biohydrogenation of linolenic acid produced cis-9, trans-11, cis-15 C18:3 and trans-11, cis-15 C18:2 as intermediates and vaccenic acid as the end product of biohydrogenation. Oleic acid and stearic acid were not converted to any other fatty acid. It is concluded that pathways for biohydrogenation of linoleic and linolenic acids by Orpinomyces are the same as those for group A rumen bacteria.

Keywords

Rumen Fungi;Orpinomyces;Biohydrogenation;Fatty Acids

References

  1. Kepler, C. R., K. P. Hirons, J. J. McNell and S. B. Tove. 1966. Intermediates and products of the biohydrogenation of linoleic acid by Butyrivibrio fibrisolvens. J. Biol. Chem. 241:1350-1354.
  2. Kim, T. W., N. J. Choi, J. Hwangbo, J. T. Hsu, S. S. Lee, M. K. Song, I. J. Seo and Y. J. Kim. 2005. Production of trans-10, cis-12 conjugated linoleic acid by Megasphaera Elsdenii YJ-4: physiological roles in the rumen. Asian-Aust. J. Anim. Sci. 18:1425-1429. https://doi.org/10.5713/ajas.2005.1425
  3. Kemp, P., R. W. White and D. J. Lander. 1975. The hydrogenation of unsaturated fatty acids by five bacterial isolates from the sheep rumen, including a new species. J. Gen. Microbiol. 90:100-114. https://doi.org/10.1099/00221287-90-1-100
  4. Kemp, P. and D. J. Lander. 1984. Hydrogenation in vitro of ${\alpha}-linolenic$ acid to stearic acid by mixed culture of pure strains of rumen bacteria. J. Gen. Microbiol. 130:527-533.
  5. Kemp, P., D. J. Lander and C. G. Orpin. 1984. The lipids of rumen fungus Piromonas communis. J. Gen. Microbiol. 130:27-37.
  6. Kemp, P. and D. J. Lander. 1983. The hydrogenation of ${\gamma}-linolenic$ acid by pure cultures of two rumen bacteria. Biochem. J. 216:519-522. https://doi.org/10.1042/bj2160519
  7. Joblin, K. N. 1981. Isolation, enumeration and maintenance of rumen anaerobic fungi in roll tubes. Appl. Environ. Microbiol. 42:1119-1122.
  8. Wang, J. H., S. H. Choi, C. G. Yan and M. K. Song. 2005. Effect of monensin and fish oil supplementation on biohydrogenation and CLA production by rumen bacteria in vitro when incubated with safflower oil. Asian-Aust. J. Anim. Sci. 18:221-225. https://doi.org/10.5713/ajas.2005.221
  9. Wang, J. H., S. H. Choi, K. W. Lim, K. H. Kim and M. K. Song. 2006. Effect of the mixed oil and monensin supplementation, and feeding duration of supplements on c9,t11-CLA contents in plasma and fat tissues of Koran native (Hanwoo) steers. Asian-Aust. J. Anim. Sci. 19:1464-1469. https://doi.org/10.5713/ajas.2006.1464
  10. Wang, J. H. and M. K. Song. 2003. pH affects the in vitro formation of cis-9, trans-11 CLA and octadecenoic acid by rumen bacteria when incubated with oilseeds. Asian-Aust. J. Anim. Sci. 16:1743-1748. https://doi.org/10.5713/ajas.2003.1743
  11. Sachan, D. S. and C. L. Davis. 1969. Hydrogenation of linoleic acid by spirochete. J. Bacteriol. 98:300-301.
  12. Song, M. K. and J. J. Kennelly. 2003. Biosynthesis of conjugated linoleic acid and its incorporation into ruminant's products. Asian-Aust. J. Anim. Sci. 16:306-314. https://doi.org/10.5713/ajas.2003.306
  13. Noble, R. C., W. Steel and J. H. Moore. 1969. The incorporation of linoleic acid into the plasma lipids of sheep given intraruminal infusions of maize oil or free linoleic acid. Br. J. Nutr. 23:709-714. https://doi.org/10.1079/BJN19690079
  14. Kim, Y-J., R. H. Liu, D. Bond and J. B. Russell. 2000. The effect of linoleic acid concentration on the conjugated linoleic acid (CLA) production of Butyrivibrio fibrisolvens A38. Appl. Environ. Microbiol. 66:5226-5230. https://doi.org/10.1128/AEM.66.12.5226-5230.2000
  15. Nam, I. S. and P. C. Garnsworthy. 2007. Biohydrogenation of linoleic acid by rumen fungi compared with rumen bacteria. J. Appl. Microbiol. 103:551-556. https://doi.org/10.1111/j.1365-2672.2007.03317.x
  16. Zhang, Y., X. Kong, X. Zhu, R. Wang, Y. Yan and Z. Jia. 2006. Effect of forage to concentrate ratio and monensin supplementation on cis-9, trans-11 conjugated linoleic acid and trans-11 octadecenoic acid concentrations of ruminal contents and plasma in sheep. Asian-Aust. J. Anim. Sci. 19:699-704. https://doi.org/10.5713/ajas.2006.699
  17. Yokoyama, M. T. and C. L. Davis. 1971. Hydrogenation of unsaturated fatty acids by Treponema (Borrelia) strain $B_25$ a rumen spirochaete. J. Bacteriol. 107:519-527.
  18. Bauchop, T. 1979. Rumen anaerobic fungi of cattle and sheep. Appl. Environ. Microbiol. 38:148-158.
  19. Choi, S. H., J. H. Wang., Y. J. Kim, Y. K. Oh and M. K. Song. 2006. Effect of soybean oil supplementation on the contents plasma cholesterol and cis9, trans11-CLA of the fat tissues in sheep. Asian-Aust. J. Anim. Sci. 19:679-683. https://doi.org/10.5713/ajas.2006.679
  20. Chillard, Y., A. Frelay, R. M. Mansbridge and M. Doreau. 2000. Ruminant milk fat plasticity: nutritional control of saturated, polyunsaturated, trans and conjugated fatty acids. Ann. Zootech. 49:181-205. https://doi.org/10.1051/animres:2000117
  21. Body, D. R. and T. Bauchop. 1985. Lipid composition of an anaerobic fungus, Neocallimastix frontalis, isolated from bovine rumen. Can. J. Microbiol. 31:464-466.
  22. Chouinard, P. Y., L. Corneau, A. Saebo and D. E. Bauman. 1999. Milk yield and composition during abomasal infusion of conjugated linoleic acids in dairy cows. J. Dairy Sci. 82:2737-2745. https://doi.org/10.3168/jds.S0022-0302(99)75530-X
  23. Choi, S. H. and M. K. Song. 2005. Effect of C18-polyunsaturated fatty acids on their direct incorporation into the rumen bacterial lipids and CLA production in vitro. Asian-Aust. J. Anim. Sci. 18:512-515. https://doi.org/10.5713/ajas.2005.512
  24. Christie, W. W. 1982. A simple procedure for the rapid trans methylation of glycerolipids and cholesteryl esters. J. Lipid Res. 23:1072-1075.
  25. Dawson, R. M. C. and P. Kemp. 1970. Biohydrogenation of dietary fats in ruminants. In: Physiology of Digestion and Metabolism in the Ruminant (Ed. A. T. Phillipson). Oriel Press. Newcastle- upon-Tyne, pp. 504-518.
  26. Feng, S., A. L. Lock and P. C. Garnsworthy. 2004. A rapid lipid separation method for determining fatty acid composition of milk. J. Dairy Sci. 87:3785-3788. https://doi.org/10.3168/jds.S0022-0302(04)73517-1
  27. Eyssen, H. and A. Verhulst. 1984. Biotransformation of linoleic acid and bile acids by Eubacterium lentum. Appl. Environ. Microbiol. 47:39-42.
  28. Hungate, R. E. 1969. A roll tube method for cultivation of strict anaerobes. In Methods in Microbiology (Ed. J. R. Norris and W. D. Ribbons). Academic Press, Inc., London, pp. 117-132.
  29. Hazlewood, G. P., P. Kemp, D. Lander and R. M. C. Dawson. 1976. $C_{18}$ unsaturated fatty acid hydrogenation patterns of some rumen bacteria and their ability to hydrolyse exogenous phospholipid. Br. J. Nutr. 35:293-297. https://doi.org/10.1079/BJN19760034
  30. Hara, I. B. and N. S. Radin. 1978. Lipid extraction of tissues with a low-toxicity solvent. Anal. Biochem. 90:420-426. https://doi.org/10.1016/0003-2697(78)90046-5

Cited by

  1. Effect of Sunflower and Marine Oils on Ruminal Microbiota, In vitro Fermentation and Digesta Fatty Acid Profile vol.8, pp.1664-302X, 2017, https://doi.org/10.3389/fmicb.2017.01124
  2. Symposium review: Understanding diet–microbe interactions to enhance productivity of dairy cows vol.101, pp.8, 2018, https://doi.org/10.3168/jds.2017-13858