Human Erythropoietin Induces Lung Failure and Erythrocytosis in Transgenic Mice

  • Kim, Myoung Ok (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Sung Hyun (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Shin, Mi Jung (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Dong Beom (The Republic of Korea Red Cross, Nam-Bu Seoul Blood Center) ;
  • Kim, Tae Won (Department of Life Science, Dongguk University) ;
  • Kim, Kil Soo (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Ha, Ji Hong (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Sanggyu (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Park, Yong Bok (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University) ;
  • Kim, Sun Jung (Department of Life Science, Dongguk University) ;
  • Ryoo, Zae Young (School of Life Sciences and Biotechnology, College of Natural Sciences, Kyungpook National University)
  • Received : 2006.09.06
  • Accepted : 2006.11.16
  • Published : 2007.02.28

Abstract

We have expressed human erythropoietin (EPO) in transgenic mice using a recombinant EPO cDNA combined with a partial TPO construct. The gene was microinjected using standard techniques and five mice were detected as transgenic by PCR and further used as founders. The life span of the transgenic founders was much shorter than that of their normal littermates. Most of the tissues of the transgenic founders contained human EPO transcripts as judged by RT-PCR. Especially high expression levels were seen in the liver and lung. EPO protein levels in serum were examined by ELISA and ranged from 266-414 mIU/ml. The number of red blood cell, white blood cell and hemoglobin in the hEPO transgenic mice was higher than in normal mice. These results indicate that overexpression of hEPO is deleterious and can provoke lung failure and erythrocytosis.

Keywords

Erythrocytosis;Erythropoietin;Lung Failure;Thrombopoietin

Acknowledgement

Supported by : The Korea Ministry of Agriculture and Fisheries, Korea Science and Engineering Foundation

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