Mining of Biomarker Genes from Expressed Sequence Tags and Differential Display Reverse Transcriptase-Polymerase Chain Reaction in the Self-fertilizing Fish, Kryptolebias marmoratus and Their Expression Patterns in Response to Exposure to an Endocrine-disrupting Alkylphenol, Bisphenol A

  • Lee, Young-Mi (Department of Chemistry, and the National Research Lab of Marine Molecular and Environmental Bioscience, College of Natural Sciences, Hanyang University) ;
  • Rhee, Jae-Sung (Department of Molecular and Environmental Bioscience, and the National Research Lab of Marine Molecular and Environmental Bioscience, Graduate School, Hanyang University) ;
  • Hwang, Dae-Sik (Department of Molecular and Environmental Bioscience, and the National Research Lab of Marine Molecular and Environmental Bioscience, Graduate School, Hanyang University) ;
  • Kim, Il-Chan (Polar BioCenter, Korea Polar Research Institute, Korea Ocean Research and Development Institute) ;
  • Raisuddin, Sheikh (Department of Chemistry, and the National Research Lab of Marine Molecular and Environmental Bioscience, College of Natural Sciences, Hanyang University) ;
  • Lee, Jae-Seong (Department of Chemistry, and the National Research Lab of Marine Molecular and Environmental Bioscience, College of Natural Sciences, Hanyang University)
  • Received : 2006.11.22
  • Accepted : 2007.02.20
  • Published : 2007.06.30

Abstract

Expressed sequence tags (ESTs) and differentially expressed cDNAs from the self-fertilizing fish, Kryptolebias marmoratus were mined to develop alternative biomarkers for endocrine-disrupting chemicals (EDCs). 1,577 K. marmoratus cDNA clones were randomly sequenced from the 5'-end. These clones corresponded to 1,518 and 1,519 genes in medaka dbEST and zebrafish dbEST, respectively. Of the matched genes, 197 and 115 genes obtained Unigene IDs in medaka dbEST and zebrafish dbEST, respectively. Many of the annotated genes are potential biomarkers for environmental stresses. In a differential display reverse transcriptase-polymerase chain reaction (DD RT-PCR) study, 56 differential expressed genes were obtained from fish liver exposed to bisphenol A. Of these, 16 genes were identified after BLAST search to GenBank, and the annotated genes were mainly involved in catalytic activity and binding. The expression patterns of these 16 genes were validated by real-time RT-PCR of liver tissue from fish exposed to bisphenol A. Our findings suggest that expression of these 16 genes is modulated by endocrine disrupting chemicals, and therefore that they are potential biomarkers for environmental stress including EDCs exposure.

Keywords

Biomarker Genes;Differential Display Reverse Transcriptase Polymerase Chain Reaction;Expressed Sequence Tags;Kryptolebias marmoratus;Self-fertilizing Fish

Acknowledgement

Supported by : Korea Research Foundation ABRL

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