Expression of a Functional Type-I Chalcone Isomerase Gene Is Localized to the Infected Cells of Root Nodules of Elaeagnus umbellata

  • Kim, Ho Bang (School of Biological Sciences, Seoul National University) ;
  • Bae, Ju Hee (School of Biological Sciences, Seoul National University) ;
  • Lim, Jung Dae (Department of Herbal Medicine Resource, Kangwon National University) ;
  • Yu, Chang Yeon (Division of Applied Plants Science, Kangwon National University) ;
  • An, Chung Sun (School of Biological Sciences, Seoul National University)
  • Received : 2007.03.19
  • Accepted : 2007.04.09
  • Published : 2007.06.30


A putative type-I chalcone isomerase (CHI) cDNA clone EuNOD-CHI was previously isolated from the root nodule of Elaeagnus umbellata [Kim et al. (2003)]. To see if it encodes a functional CHI, we ectopically overexpressed it in the Arabidopsis (Arabidopsis thaliana) transparent testa 5 (tt5) mutant, which is defective in naringenin production and has yellow seeds due to proanthocyanidin deficiency. Ectopic overexpression of EuNOD-CHI resulted in recovery of normal seed coat color. Naringenin produced by CHI from naringenin chalcone was detected in the transgenic lines like in the wild-type, whereas it was absent from the tt5 mutant. We conclude that EuNOD-CHI encodes a functional type-I CHI. In situ hybridization revealed that EuNOD-CHI expression is localized to the infected cells of the fixation zone in root nodules.


Arabidopsis tt5 Mutant;Chalcone Isomerase;Molecular Complementation;Elaeagnus umbellata;Flavonoids Analysis;Frankia;In Situ Hybridization;Root Nodule


Supported by : Korean Ministry of Science and Technology


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