DOI QR코드

DOI QR Code

Isolation and Cloning of Porcine SLC27A2 Gene and Detection of Its Polymorphism Associated with Growth and Carcass Traits

  • Wang, Tao (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University) ;
  • Liu, Chang (Department of Food Engineering, Hebei Normal University of Science & Technology) ;
  • Xiong, Yuan-Zhu (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University) ;
  • Deng, Chang-Yan (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University) ;
  • Zuo, Bo (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University) ;
  • Xie, Hong-Tao (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University) ;
  • Xu, De-Quan (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture & Key Lab of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University)
  • Received : 2006.10.11
  • Accepted : 2007.04.07
  • Published : 2007.08.01

Abstract

The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in LargeWhite and Meishan breeds showed that a single nucleotide polymorphism (SNP) ($A{\rightarrow}G$) existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ile, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 $F_2$ reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.

Keywords

Solute Carrier Family 27;Member 2;Pig;Polymorphism

Acknowledgement

Supported by : Natural Science Foundation of Hubei Province

References

  1. Mootha, V. K., J. Bunkenborg, J. V. Olsen, M. Hjerrild, J. R. Wisniewski, E. Stahl, M. S. Bolouri, H. N. Ray, S. Sihag, M. Kamal, N. Patterson, E. S. Lander and M. Mann. 2003. Integrated analysis of protein composition, tissue diversity, and gene regulation in mouse mitochondria. Cell, 115:629-640. https://doi.org/10.1016/S0092-8674(03)00926-7
  2. Niu, B. Y., Y. Z. Xiong, S. W. Jiang, F. E. Li, C. Y. Deng, S. H. Ding, W. H. Guo, M. G. Lei, R. Zheng, B. Zuo, D. Q. Xu and J. L. Li. 2006. Oviduct-specific Glycoprotein 1 Locus is Associated with Litter Size and Weight of Ovaries in Pigs. Asian-Aust. J. Anim. Sci. 19:632-637. https://doi.org/10.5713/ajas.2006.632
  3. Schaffer, J. E. and H. F. Lodish. 1994. Expression cloning and characterization of a novel adipocyte long chain fatty acid transport protein. Cell, 79:427-436. https://doi.org/10.1016/0092-8674(94)90252-6
  4. Hirsch, D., A. Stahl and H. F. Lodish. 1998. A family of fatty acid transporters conserved from mycobacterium to man. Proceedings of the National Academy of Sciences USA. 95:8625-8629. https://doi.org/10.1073/pnas.95.15.8625
  5. Krzecio, E., J. Kuryl, Kocwin, M. Podsiadls and G. Monin. 2005. Association of calpastatin (CAST/MspI) polymorphism with meat quality parameters of fatteners and its interaction with RYR1 genotypes. J. Anim. Breed Genet. 122:251-258. https://doi.org/10.1111/j.1439-0388.2005.00517.x
  6. Liu, B. H. 1998. Statistical Genomics: Linkage, Mapping, and QTL Analysis. CRC Press, LLC, 404-409.
  7. Hamilton, D. N., M. Ellis, K. D. Miller, F. K. McKeith and D. F. Parrett. 2000. The effect of the Halothane and Rendement Napole genes on carcass and meat quality characteristics of pigs. J. Anim. Sci. 78:2862-2867. https://doi.org/10.2527/2000.78112862x
  8. Heinzer, A. K., S. Kemp, J. F. Lu, P. A. Watkins and K. D. Smith. 2002. Mouse very long-chain acyl-CoA synthetase in X-linked adrenoleukodystrophy. J. Biol. Chem. 277:28765-28773. https://doi.org/10.1074/jbc.M203053200
  9. Berger, J., C. Truppe, H. Neumann and S. Forss-Petter. 1998. cDNA cloning and mRNA distribution of a mouse very longchain acyl-CoA synthetase. FEBS Lett, 425:305-309. https://doi.org/10.1016/S0014-5793(98)00255-5
  10. Fuji, J., K. Otsu and F. Zorzto. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Sci. 253:448-451. https://doi.org/10.1126/science.1862346
  11. Andersson, L. 2003. Identification and characterization of AMPK gamma 3 mutations in the pig. Biochem. Soc. Trans. 31:232-235 https://doi.org/10.1042/BST0310232
  12. Zuo, B., Y. Z. Xiong, Y. H. Su, C. Y. Deng, R. Zheng and S. W. Jiang. 2003. Mapping quantitative trait loci meat quality on pig chrosomone 3, 4 and 7. Asian-Aust. J. Anim. Sci. 16:320-324. https://doi.org/10.5713/ajas.2003.320
  13. Zuo, B., Y. Z. Xiong, C. Y. Deng, Y. H. Su, J. Wang, M. G. Lei, F. E. Li, S. W. Jiang and R. Zheng. 2005. Polymorphism, linkage mapping and expression pattern of the porcine skeletal muscle glycogen synthase (GYS1) gene. Anim. Genet. 36:254-257. https://doi.org/10.1111/j.1365-2052.2005.01286.x
  14. Uchiyama, A., T. Aoyama, K. Kamijo, Y. Uchida, N. Kondo, T. Orii and T. Hashimoto. 1996. Molecular cloning of cDNA encoding rat very long-chain acyl-CoA synthetase. J. Biol. Chem. 271:30360-30365. https://doi.org/10.1074/jbc.271.48.30360
  15. Wakui, K., T. Aoyama, A. Uchiyama, T. Hashimoto and Y. Fukushima. 1998. Assignment of human fatty-acid-Coenzyme A ligase, very long-chain 1 gene (FACVL1) to human chromosome band 15q21.2 by fluorescence in situ hybridization. Cytogenetics and Cell Genetics, 81:292-293. https://doi.org/10.1159/000015051
  16. Xiong, Y. Z. and C. Y. Deng. 1999. Principle and Method of Swine Testing. Chinese Agriculture Press, Beijing (in Chinese).
  17. Berger, J., C. Truppe, H. Neumann and S. Forss-Petter. 1998. A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern. Biochem. Biophys. Res. Commun. 247:255-260. https://doi.org/10.1006/bbrc.1998.8770

Cited by

  1. Dietary Supplementation with Trihexanoin Enhances Intestinal Function of Weaned Piglets vol.19, pp.10, 2018, https://doi.org/10.3390/ijms19103277