Dietary Supplementation with Acanthopanax senticosus Extract Modulates Cellular and Humoral Immunity in Weaned Piglets

  • Kong, Xiangfeng (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Yin, Yulong (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Wu, Guoyao (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Liu, Hejun (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Yin, Fugui (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Li, Tiejun (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Huang, Ruilin (Laboratory of Animal Nutrition and Human Health and Key Laboratory of subtropical Agro-ecology the Chinese Academy of Sciences) ;
  • Ruan, Zheng (Key Laboratory of Food Science of Ministry of Education, Department of Food Science and Engineering, Nanchang University) ;
  • Xiong, Hua (Key Laboratory of Food Science of Ministry of Education, Department of Food Science and Engineering, Nanchang University) ;
  • Deng, Zeyuan (Key Laboratory of Food Science of Ministry of Education, Department of Food Science and Engineering, Nanchang University) ;
  • Xie, Mingyong (Key Laboratory of Food Science of Ministry of Education, Department of Food Science and Engineering, Nanchang University) ;
  • Liao, Yiping (Guangzhou Tanke Industry Co. Ltd.) ;
  • Kim, Sungwoo (Department of Animal and Food Sciences, Texas Tech University)
  • Received : 2006.12.29
  • Accepted : 2007.04.06
  • Published : 2007.09.01


This study was conducted to test the hypothesis that dietary supplementation with an herbal extract of Acanthopanax senticosus (AS) enhances the immune response in weaned piglets. Sixty piglets weaned at 21 days of age were randomly assigned to 3 treatment groups representing the addition of 0 or 1 g/kg of the AS extract or 0.2 g/kg of colistin (an antibiotic) to maize- and soybean meal-based diets (n = 20 per group). On days 7, 14 and 28 after initiation of the addition, total and differential counts of leucocytes, proliferating activity of peripheral lymphocytes, serum levels of immunoglobulins (Ig) and cytokines and the spleen index were determined. The AS extract decreased (p<0.05) the number of neutrophils on days 7 and 28 in comparison with the control group and reduced (p<0.05) serum interleukin-$1{\beta}$ level on day 28 compared with the other 2 groups. Dietary supplementation with the AS extract increased (p<0.05) the lymphocyte/leukocyte ratio on day 28 compared with the control group and increased the proliferating activity of lymphocytes on days 14 and 28 compared with the other 2 groups. The AS extract increased (p<0.05) the serum content of IgG on day 7 and of IgG and IgM on day 28 compared with the other 2 groups, as well as increasing the serum content of tumor necrosis factor on day 7 and spleen index on days 7 and 28 compared with the control group. Collectively, these findings suggest that the AS extract as a dietary additive enhances the cellular and humoral immune responses of weaned piglets by modulating the production of immunocytes, cytokines and antibodies.


Supported by : National Natural Science Foundation of China


  1. Yin, Y. L., R. L. Huang, H. Y. Zhong, T. J. Li, W. B. Souffrant and C. F. M. de Lange. 2002. Evaluation of mobile bag technique for determining apparent ileal digestibilities of crude protein and amino acids in growing pig. J. Anim. Sci. 80:409-420
  2. Yin, Y.-L., Z.-Y. Deng, H.-L. Huang, T.-J. Li and H.Y. Zhong.2004. The effect of arabinoxylanase and protease supple-mentation on nutritional value of diets containing wheat bran or rice bran in growing pigs. J. Anim. Feed Sci. 13:445-461
  3. Yin, Y.-L., S. K. Baidoo, Z. L. Jin, Y. G. Liu, H. Schulze and P. H. Simmins. 2001c.The effect of different carbohydrase and protease supplementation on apparent (ileal and overall) digestibility of nutrient of five hulless barley varieties in young pigs. Livest. Prod. Sci. 71:109-120
  4. Zhou, J., B. C. Wang and Y. Wang. 2005. Determination and evaluation of saccharides content in extract of herba spirodelae seu lemnae polysaccharides. J. Chongqing Univ. (Natural Science Edition) 28:111-114
  5. Wu, G., D. A. Knabe and S. W. Kim. 2004. Arginine nutrition in neonatal pigs. J. Nutr. 134:2783S-2390S
  6. Wu, G., N. E. Flynn, D. A. Knabe and L. A. Jaeger. 2000. A cortisol surge mediates the enhanced polyamine synthesis in porcine enterocytes during weaning. Am. J. Physiol. 279:R554-R559
  7. Wu, G., S. A. Meier and D. A. Knabe. 1996. Dietary glutamine supplementation prevents jejunal atrophy in weaned pigs. J. Nutr. 126:2578-2584
  8. Yang, C. H., G. Liu, C. X. Zhang and Y. L. Zheng. 2004. Progress of study on acanthopanax. Panax Res. 1:17-21
  9. Yang, H., A. K. Li, Y. L. Yin, T. J. Li, Z. R. Wang, G. Wu, R. L. Huang, X. F. Kong, C. B. Yang, P. Kang, J. Deng, S. X. Wang, B. E. Tan, Q. Hu, F. F. Xing, X. Wu, Q. H. He, K. Yao, Z. J. Liu, Z. R. Tang, F. G. Yin, Z. Y. Deng, M. Y. Xie and M. Z. Fan. 2007. True phosphorus digestibility and the endogenous phosphorus outputs associated with brown rice for weanling pigs measured by the simple linear regression analysis technique weanling pigs. Anim. 1:213-220
  10. Yi, J. M., M. S. Kim, S. W. Seo, K. N. Lee, C. S. Yook and H. M. Kim. 2001. Acanthopanax senticosus root inhibits mast celldependent anaphylaxis. Clin. Chim. Acta. 312:163-168
  11. Yi, J. M., S. H. Hong, J. H. Kim, H. K. Kim, H. J. Song and H. M. Kim. 2002. Effect of Acanthopanax senticosus stem on mast cell-dependent anaphylaxis. J. Ethnopharmacol. 79(3):347-352
  12. Yin, Y. L., H. Y. Zhong, R. L. Huang, C. M. Chen, T. J. Li and Y. F. Pai. 1993. Nutritive value of feedstuffs and diets for pigs. I. Chemical composition, apparent ileal and fecal digestibility. Anim. Feed Sci. Technol. 44:1-27
  13. Yin, Y.-L., S. K. Baidoo and J. J. J. Boychul. 2001a Effect of replacing wheat and maize with micronization of dehulled barley on starter, grower and finisher pig perofrmance. Asian-Aust. J. Anim. Sci. 14:668-676
  14. Yin, Y.-L, S. K. Baidoo, H. Schulze and P. H. Simmins. 2001b. Effect of supplementing diets containing hulless barley varieties having different levels of non-starch polysaccharides with ${\beta}-glucanase$ and xylanase on the physiological status of gastrointestinal tract and nutrient digestibility of weaned pigs. Livest. Prod. Sci. 71:97-107
  15. Nishibe, S., H. Kinoshita, H. Takeda and G. Okano. 1990. Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats. Chem. Pharmaceut. Bull. 38:1763-1765
  16. Park, S. Y., S. Y. Chang, C. S. Yook and T. Nohara. 2000. New 3, 4-seco-lupane-type triterpene glycosides from Acanthopanax senticosus forma inermis. J. Nat. Prod. 63(12):1630-1633
  17. Schmolz, M. W., F. Sacher and B. Aicher. 2001. The synthesis of Rantes, G-CSF, IL-4, IL-5, IL-6, IL-12 and IL-13 in human whole-blood cultures is modulated by an extract from Eleutherococcus senticosus L. roots. Phytother. Res. 15:268- 270
  18. Sean, X. L., Q. L. Xue, Y. Huang, L. Ferrucci, L. P. Fried and J. D. Walston. 2005. Baseline total and specific differential white blood cell counts and 5-year all-cause mortality in communitydwelling older women. Exp. Gerontol. 40:982-987
  19. Tang, Z. R., Y. L. Yin, Charles M. Nyachoti, R. L. Huang, T. J. Li, C. B. Yang, X. J. Yang, J. H. Gong, J. Peng, D. S. Qi, J. J. Xing, Z. H. Sun and M. Z. Fan. 2005. Effect of dietary supplementation of chitosan and galacto-mannanoligosaccharide on serum parameters and the insulin-like growth factor-I mRNA expression in early-weaned piglets. Domist. Anim. Endocrin. 28:430-441
  20. Tanigawa, K., R. A. Craig, L. M. Stoolman and A. E. Chang. 2000. Effects of tumor necrosis factor-alpha on the in vitro maturation of tumor-reactive effector T cells. J. Immunother. 23:528-535
  21. Touchette, K. J., J. A. Carroll, G. L. Allee, R. L. Matteri, C. J. Dyer, L. A. Beausang and M. E. Zannelli. 2002. Effect of spray-dried plasma and lipopolysaccharide exposure on weaned pigs: I. Effects on the immune axis of weaned pigs. J. Anim. Sci. 80:494-501
  22. Van Heugten, E., M. T. Coffey and J. W. Spears. 1996. Effects of immune challenge, dietary energy density, and source of energy on performance and immunity in weanling pigs. J. Anim. Sci. 74:2431-2440
  23. Wang, S., Y. T. Jia and Y. Y. Sun. 1996. Study on quantitive determination of flavonoids in the flavonoids of the composite Chinese material medica preparations. WCJ.PS. 11(2):95-96
  24. Wina, E., S. Muetzel and K. Becker. 2005. The impact of saponins or saponin- containing plant materials on ruminant production: a review. J. Agric. Food Chem. 53(21):8093-8105
  25. Kusnecov, A. W. and A. Rossi-George. 2002. Stressor-induced modulation of immune function: a review of acute chronic effects in animals. Acta Neuropsychiatr. 14:279
  26. Kong, X.-F., G.-Y. Wu, Y.-L. Yin, H.-J. Liu, F.-G. Yin, T.-J. Li, R.- L. Huang, P. Kang, F.-F. Xing, M.-Z. Fan, C.-B. Yang and Q.- H. He. 2007a. Dietary supplementation with Chinese herbal ultra-fine 3 powder enhances cellular and humorl immunity in early weaned piglets. Livest. Sci. 108:94-98
  27. Kong, X. F., G. Y. Wu, Y. P. Liao, Z. P. Hou, H. J. Liu, F. G. Yin, T. J. Li, R. L. Huang, Y. M. Zhang, D. Deng, P. Kang, R. X. Wang, Z. Y. Tang, C. B. Yang, Z. Y. Deng, H. Xiong, W.-Y. Chu, Z. Yuan, M. Y. Xie and Y. L. Yin. 2007b. Effects of Chinese herbal ultra-fine powder as a dietary additive on growth performance, serum metabolites and intestinal health in early-weaned piglets. Livest. Sci. 108:272-275
  28. Li, T. J., Y. L. Yin, Y. L. Huang, X. W. Tan, Y. F. Chen and H. Y. Zhong. 2003. Effect of dietary fibre on the flow of bacterial N and bacterial amino acid at the terminal ileum of pig. Acta Vet. Zootech. Sinica. 34:239-245
  29. Li, T.-J., Y.-L. Yin, S.-Y. Bin, R.-L. Huang, Q.-Z. Dai, P. Zhang, Z.-P. Hou and H. Yang. 2007a. Growth performance and nitrogen metabolism in weaned pigs fed diets containing different sources of starch. Livest. Sci. 109:73-76
  30. Mallard, B. A., B. N. Wilkie, B. W. Kennedy, J. Gibson and M. Quinton. 1998. Immune responsiveness in swine: eight generations of selection for high and low immune response in Yorkshire pigs. Proc. 6th Wld. Congr. Genet. Appl. Livest. Prod. 27:257-264
  31. McGlone, J. and W. Pond. 2003. Pig Production: biological principles and applications. Delmar Learning, Inc., Clifton Park, NY
  32. McGlone, J. J., J. L. Salak, E. A. Lumpkin, R. I. Nicholson, M. Gibson and R. L. Norman. 1983. Shipping stress and social status effects on pig performance, plasma cortisol, natural killer cell activity, and leukocyte numbers. J. Anim. Sci. 71:888-890
  33. Medzhitov, R. and C. Janeway. 2000. Innate immune recognition: mechanisms and pathways. Immunol. Rev. 173:89-97
  34. Nabuurs, M. J. A. 1995. Microbiological, structural and functional changes of the small intestine of pigs at weaning. Pig News Inform. 16:93-97
  35. Herskin, M. S. and K. H. Jensen. 2002. Effects of open field testing and associated handling. V. Handling alone on the adrenocortical reactivity of piglets around weaning. Anim. Sci. 74:485-491
  36. Huang, R. -L., Y.-L. Yin, G.-Y. Wu, Y.-G. Zhang, T.-J. Li, L.-L. Li, M.-X. Li, Z.-R. Tang, J. Zhang, B. Wang, J.-H. He and X.-Z. Nie. 2005. Effect of Dietary Oligochitosan Supplementation on Ileal Digestibility of Nutrients and Performance in Broilers. Poult. Sci. 84:1383-1388
  37. Huang, R. L., Y. L. Yin, M. X. Li, G. Y. Wu, T. J. Li, L. L. Li, C. B. Yang, J. Zhang, B. Wang, Z. Y. Deng, Y. G. Zhang, Z. R. Tang, P. Kang and Y. M. Guo. 2007. Dietary oligochitosan supplementation enhances immune status of broilers. J. Sci. Food Agric. 87:153-159
  38. Huang, X. D., L. Kong, X. Li, X. G. Chen, M. Guo and H. F. Zou. 2004. Strategy for analysis and screening of bioactive compounds in traditional Chinese medicines. J. Chromatography B. 812:71-84
  39. Johnson, R. W. 1998. Immune and endocrine regulation of food intake in sick animals. Dom. Anim. Endocrinol. 15:309-319
  40. Jung, H. J., H. J. Park, R. G. Kim, K. M. Shin, J. Ha, J. W. Choi, H. J. Kim, Y. S. Lee and K. T. Lee. 2003. In vivo antiinflammatory and antinociceptive effects of liriodendrin isolated from the stem bar of Acanthopanax senticosus. Planta. Medica. 69:610-616
  41. Kang, J. S., P. T. Linh, X. F. Cai, H. S. Kim, J. J. Lee and Y. H. Kim. 2001. Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography. Arch. Pharm. Res. 24(5):407-411
  42. Kelley, K. W., R. W. Johnson and R. Dantzer. 1994. Immunology discovers physiology. Vet. Immunol. Immunopathol. 43:157-165
  43. King, R. H., J. L. Dividich and F. R. Dunshea. 1999. Lactation and Neonatal Growth. In: (Ed. I. Kyriazakis) A Quantitative Biology of the Pig. CABI publishing, CAB International, Wallingford, UK. pp. 155-80
  44. Kong, X. F., Y. L. Hu, R. Rui, D. Y. Wang and X. R. Li. 2004. Effects of Chinese herbal medicinal ingredients on peripheral lymphocyte proliferation and serum antibody titer after vaccination in chicken. Int. Immunopharmacol. 4:975-982
  45. Deng, D., L. Huang, T. J. Li, G. Y. Wu, M. Y. Xie, Z. R. Tang, P. Kang, Y. M. Zhang, M. Z. Fan, X. F. Kong, Z. Ruan, H. Xiong, Z. Y. Deng and Y.-L. Yin. 2007a. Nitrogen balance in barrows fed low-protein diets supplemented with essential amino acids. Livest. Sci. 109:220-223
  46. Deng, D., Ai-Ke Li, W. Y. Chu, R. L. Huang, T. J. Li, X. F. Kong, Z. J. Liu, G. Y. Wu, Y. M. Zhang and Y. L. Yin. 2007b. Growth performance and metabolic responses in barrows fed lowprotein diets supplemented with essential amino acids. Livest. Sci. 109:224-227
  47. Doherty, J. F., M. H. N. Golden, D. G. Remick and G. E. Griffin. 1994. Production of interleukin-6 and tumour necrosis factoralpha in vitro is reduced in whole blood of severely malnourished children. Clin. Sci. 86:347-351
  48. Dunkley, M., R. Pabst and A. Cripps. 1995. An important role for intestinally derived T cells in respiratory disease. Immunol. Today. 16:231-236
  49. Fan, M. Z., T. J. Li, Y. L. Yin, R. J. Fang, Z. Y. Tang, Z. P. Hou, R. L. Huang, Z. Y. Deng, H. Y. Zhong, R. G. Zhang, J. Zhang, B. Wang and H. Schulze. 2005. Effect of phytase supplementation with two levels of phosphorus diets on ileal and faecal digestibilities of nutrients and phosphorus, calcium, nitrogen and energy balances in growing pigs. Anim. Sci. 81:67-75
  50. Fujikawa, T., A. Yamaguchi, I. Morita, H. Takeda and S. Nishibe. 1996. Protective effects of Acanthopanax senticosus harms from Hokkaido and its components on gastric ulcer in restrained cold water stressed rats. Biol. Pharmaceut. Bull. 19:1227-1230
  51. Fujikawa, T., H. Soya, H. Hibasami, H. Kawashima, H. Takeda, S. Nishibe and K. Nakashima. 2002. Effect of Acanthopanax senticosus harms on biogenic monoamine levels in the rat brain. Phytother. Res. 16:474-478
  52. Greiner, L. L., T. S. Stahly and T. J. Stabel. 2000. Quantitative relationship of systemic virus concentration on growth and immune response in pigs. J. Anim. Sci. 78:2690-2695
  53. Gross, W. B. and H. S. Siegel. 1983. Evaluation of the heterophil/lymphocyte ratio and a measure of stress in chickens. Avian disease. 27:972-979
  54. Underhill, D. M. and A. Ozinsky. 2002. Phagocytosis of microbes. Annu. Rev. Immunol. 20:825-852
  55. Balaji, R., K. J. Wright, C. M. Hill, S. S. Dritz, E. L. Knoppel and J. E. Minton. 2000. Acute phase responses of pigs challenged orally with Salmonella typhimurium. J. Anim. Sci. 78:1885-1891
  56. Beers-Schreurs, H. M. G., L. Vellenga, T. H. Wensing and H. J. Breukink. 1992. The pathogenesis of the post-weaning syndrome in weaned piglets: a review. Vet. Quart. 14:29-34
  57. Bogdan Petrunov, Snejina Marinova, Roumiana Markova, Plamen Nenkov, Sasha Nikolaeva, Maria Nikolova, Hristo Taskov and Jordan Cvetanov. 2006. Cellular and humoral systemic and mucosal immune responses stimulated in volunteers by an oral polybacterial immunomodulator "Dentavax". Int. Immunopharmacol. 6(7):1181-1193
  58. Burg, N. D. and M. H. Pillinger. 2001. The neutrophil: function and regulation in innate and humoral immunity. Clin. Immunol. 99:7-17
  59. Cai, S. F., H. Y. Jiang and Y. P. Ou. 2000. Study on organic acid content in different processed products of fructus crataegi. Chinese Traditional Patent Med. 22(12):841-842
  60. Bemelmans, M. H. A., L. J. H. Van Tits and W. A. Buurman. 1996. Tumor necrosis factor: function, release and clearance. Crit. Rev. Immunol. 16:1-11
  61. Smith, R. 1999. Antibiotic bans, regulations may stop development of drugs. Feedstuffs. 71:1
  62. Han, S. B., Y. D. Yoon, H. J. Ahn, H. S. Lee, C. W. Lee, W. K. Yoon, S. K. Park and H. M. Kim. 2003. Toll-like receptormediated activation of B cells and macrophages by polysaccharide isolated from cell culture of Acanthopanax senticosus. Int. Immunopharmacol. 3(9):1301-1312
  63. Clapperton, M., S. C. Bishop and E. J. Glass. 2005. Innate immune traits differ between Meishan and Large White pigs. Vet. Immunol. Immunopathol. 104:131-144
  64. Li, L.-L., Y. -L. Yin, Y.-H. Liu, D.-X. Hou, Z.-P. Hou, C.-B Yang and X.-J. Yang. 2007b. Effects of metallothionein on antioxidative function and related enzyme activities in blood and liver, and pork meat quality. Asian-Aust. J. Anim. Sci. 20:761-767

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