Physicochemical Properties of Calcium Phosphate Thin Films Prepared in the Presence of Magnesium and Its Effects on Cellular Proliferation

마그네슘 존재 하에 형성된 칼슘포스페이트 박막의 물리화학적 특성과 세포증식에 미치는 영향

  • Shin, Hyun-Young (Laboratory of Biomaterials & Biointerface Research, Department of Chemical Engineering, Institute of Advances in Science and Technology, Dankook University) ;
  • Lee, Woo-Kul (Laboratory of Biomaterials & Biointerface Research, Department of Chemical Engineering, Institute of Advances in Science and Technology, Dankook University)
  • 신현영 (생체계면조직공학 연구실, 화학공학과, 미래과학기술연구소, 단국대학교) ;
  • 이우걸 (생체계면조직공학 연구실, 화학공학과, 미래과학기술연구소, 단국대학교)
  • Received : 2007.03.07
  • Accepted : 2007.04.16
  • Published : 2007.06.10

Abstract

The effect of the presence of magnesium ions in the preparation of calcium phosphate (CP) thin films on the physicochemical and biological properties of the films has been investigated in this study. Five different surfaces were used and the culture plate (CTL) and CP film prepared in the absence of magnesium (CaP) were used for the comparison. Three different films were prepared at different magnesium concentrations. CP films prepared at the Mg concentrations of 0.1, 1, and 10 mM were designated as CaPL, CaPM, and CaPH, respectively. The observation of surface morphology of the CP films using scanning electron microscopy (SEM) displayed that the presence of magnesium affected considerably the morphology of the films including a decrease of surface porosity. X-ray diffraction (XRD) analysis for the determination of the crystallinity of the CP films showed that the structure of the films was amorphous. Examination using X-ray photoelectron spectroscopy (XPS) confirmed the prepared films consisted of calcium, phophorus, and magnesium. Cell adhesion assays elucidated that cell adhered less as the magnesium concentration increased. However, cell proliferation assays demonstrated that the cells proliferated more actively on the films prepared at the higher magnesium concentration. These results suggest that the presence of magnesium may promote the biocompatibility of CP films.

Keywords

calcium phosphate;thin film;magnesium;osteoblast;XPS;XRD;SEM

Acknowledgement

Supported by : 단국대학교

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