Determination of diallyl disulfide in garlic by reversed-phase high performance liquid chromatography

역상 고성능 액체 크로마토그래피를 이용한 마늘에서 diallyl disulfide의 분석

  • Wan, Xiaolong (Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University) ;
  • Polyakova, Yulia (Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University) ;
  • Row, Kyung Ho (Center for Advanced Bioseparation Technology and Dept. of Chem. Eng., Inha University)
  • 만효룡 (초정밀생물분리기술연구센터, 화공과, 인하대학교) ;
  • ;
  • 노경호 (초정밀생물분리기술연구센터, 화공과, 인하대학교)
  • Received : 2007.07.16
  • Accepted : 2007.08.28
  • Published : 2007.10.25


The properties of garlic (Allium sativum L.) are attributed to organosulfur compounds. In this paper, an analytical technique with a rapid and simple sample preparation procedures for determination of diallyl disulfide (DADS) in garlic was reported. The DADS was simply extracted with various solvents (methanol, benzene or tetrahydrofuran) from garlic and prepared for HPLC analysis. From the results, the methanol was select as an optimal extraction solvent. The mobile phase was composed from methanol and water, and the gradient elution mode was applied. 0.61 mg of DADS per g garlic powder can be extracted with methanol. This work offers some advantages over the currently accepted techniques and would be useful for chemical and biological studies of garlic and its products.


Supported by : Inha University


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