Transgenic Tobacco Expressing the hrpNEP Gene from Erwinia pyrifoliae Triggers Defense Responses Against Botrytis cinerea

  • Sohn, Soo-In ;
  • Kim, Yul-Ho ;
  • Kim, Byung-Ryun ;
  • Lee, Sang-Yeob ;
  • Lim, Chun Keun ;
  • Hur, Jang Hyun ;
  • Lee, Jang-Yong
  • Received : 2007.03.23
  • Accepted : 2007.05.28
  • Published : 2007.10.31

Abstract

$HrpN_{EP}$, from the gram-negative pathogen, Erwinia pyrifoliae, is a member of the harpin group of proteins, inducing pathogen resistance and hypersensitive cell death in plants. When the $hrpN_{EP}$ gene driven by the OsCc1 promoter was introduced into tobacco plants via Agrobacterium-mediated transformation, their resistance to the necrotrophic fungal pathogen, Botrytis cinerea, increased. Resistance to B. cinerea was correlated with enhanced induction of SA-dependent genes such as PR-1a, PR2, PR3 and Chia5, of JA-dependent genes such as PR-1b, and of genes related to ethylene production, such as NT-EFE26, NT-1A1C, DS321, NT-ACS1 and NT-ACS2. However the expression of NPR1, which is thought to be essential for multiple-resistance, did not increase. Since the pattern of expression of defense-related genes in $hrpN_{EP}$-expressing tobacco differed from that in plants expressing $hpaG_{Xoo}$ from Xanthomonas oryzae pv. Oryzae, these results suggest that different harpins can affect the expression of different defense-related genes, as well as resistance to different plant pathogens.

Keywords

Erwinia pyrifoliae;$hrpN_{EP}$;NPR1;SA

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Acknowledgement

Supported by : RDA