Genetic Diversity of Wild Quail in China Ascertained with Microsatellite DNA Markers

  • Chang, G.B. (Animal Science & Technology College, Yangzhou University) ;
  • Chang, H. (Animal Science & Technology College, Yangzhou University) ;
  • Liu, X.P. (Animal Science & Technology College, Yangzhou University) ;
  • Zhao, W.M. (Animal Science & Technology College, Yangzhou University) ;
  • Ji, D.J. (Animal Science & Technology College, Yangzhou University) ;
  • Mao, Y.J. (Animal Science & Technology College, Yangzhou University) ;
  • Song, G.M. (Animal Science & Technology College, Yangzhou University) ;
  • Shi, X.K. (Animal Science & Technology College, Yangzhou University)
  • Received : 2006.09.13
  • Accepted : 2007.04.07
  • Published : 2007.12.01


The genetic diversity of domestic quail and two wild quail species, Japanese (Coturnix coturnix)and Common quail (Coturnix japonica), found in China was studied using microsatellite DNA markers. According to a comparison of the corresponding genetic indices in the three quail populations, such as Polymorphism Information Content (PIC), Mean Heterozygosity ($\bar{H}$) and Fixation Index, wild Common quail possessed rich genetic diversity with 4.67 alleles per site. Its values for PIC and $\bar{H}$ were the highest, 0.5732 and 0.6621, respectively. Domestic quail had the lowest values, 0.5467 and 0.5933, respectively. Wild Japanese quail had little difference in genetic diversity from domestic quail. In addition, from analyses of the fuzzy cluster based on standard genetic distance, the similarity relationship matrix coefficient between wild Japanese quail and domestic quail was 0.937, and that between wild Common quail and domestic quail was 0.783. All of these results showed that the wild Japanese quail were closer to the domestic quail for phylogenetic relationship than wild Common quail. These results at the molecular level provide useful data about quail's genetic background and further supported the hypothesis that the domestic quail originated from the wild Japanese quail.


Supported by : National Natural Science Foundation of China


  1. Bostein, D., R. L. White, M. Sckolnick and R. W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32:317-331.
  2. Cao, H. H., Y. C. Wang, Y. C. Chen, Y. Zhang and L. Ferretti. 1999. Study on five microsatellite markers in beef cattle population. Scientia Agricultura Sinica. 32(1):69-73.
  3. Chang, G. B., H. Chang, X. P. Liu, W. M. Zhao and O. Olowofeso. 2005. Developmental research on origin and phylogeny of quail. World's Poult. Sci. J. 61(1):105-112.
  4. Chang, G. B., H. Chang, H. L. Zhen, X. P. Liu, W. Sun, R. Q. Geng, Y. M. Yu, S. C. Wang, S. M. Geng, X. L. Liu, G. Q. Qin and W. Shen. 2001.Study on phylogenetic relationship between wild Japanese quails in the Weishan Lake area and domestic quails. Asian-Aust. J. Anim. Sci. 14(5):603-607.
  5. Chang, H. 1998. In: Study on Animal Genetic Resources of China. Xian: Shaanxi People's Education Publishing House, China. pp. 243-249
  6. Chang, H., K. Nozawa, X. L. Liu, S. M. Geng, Z. J. Ren, G. Q. Qin, X. G. Li, J. M. Sun, H. L. Zheng, J. Z. Song, Y. Kurosawa, A. Sano, Q. Jia and G. H. Chen. 2000. Study on phylogenetic relationships among native goat populations along the middle and lower yellow river valley. Asian-Aust. J. Anim. Sci. 13(2):137-148.
  7. Chang, H., K. Nozawa, X. L. Liu, S. M. Geng, Z. J. Ren and G. Q. Qin. 2000. Study on phylogenetic relationship of native goat populations along the middle and lower yellow river valley. Scientia Agricultura Sinica 33(3):79-87.
  8. Fan, B., K. Li, Z. Z. Peng, Y. Chen, C. Moran, Y. C. Gong and S. H. Zhao. 1999. Genetic variation of 27 microsatellite sites in three Hubei indigenous pig breeds. Chinese Biodiversity 7(2): 91-96.
  9. Inoue-Murayama, M., B. B. Kayang, K. Kimura, H. Ide, A. Nomura, H. Takahashi, Y. Nagamine, T. Takeda, H. Hanada, K. Tatsuda, M. Tsudzuki, Y. Matsuda, M. Mizutani, Y. Murayama and S. Ito. 2001.Chicken microsatellite primers are not efficient markers for Japanese quail. Anim. Genet. 32:7-11.
  10. Inoue-Murayama, M. and A. Nomura. 1998. Application of chicken microsatellite markers to Japanese quail and Chinese painted quail. Anim. Genet. 29 (Suppl.1):46.
  11. Kayang, B. B., M. Inoue-Murayama, T. Hoshi, K. Matsuo, H. Takahashi, M. Minezawa, M. Mizutani and S. Ito. 2002. Microsatellite sites in Japanese quail and cross-species amplification in chicken and guinea fowl. Genet. Sel. Evol. 34:233-253.
  12. Kayang, B. B., M. Inoue-Murayama, A. Nomura, K. Kimura, H. Takahashi, M. Mizutani and S. Ito. 2000. Fifty microastellite markers for Japanese quail. J. Heted. 91(6):502-505.
  13. Kimura, M. 1996. Wild quails. Study on Animal Products. 50:197-202.
  14. Kimura, M. and T. Ohta. 1973. Mutation and evolution at the molecular level. Genet. 73 (Suppl):19-35.
  15. Kong, H. S., J. D. Oh, J. H. Lee, K. J. Jo, B. D. Sang, C. H. Choi, S. D. Kim, S. J. Lee, S. H. Yeon, G. J. Jeon and H. K. Lee. 2006. Genetic variation and relationships of Korean native chickens and foreign breeds using 15 microsatellite markers. Asian-Aust. J. Anim. Sci. 19(11):1546-1550.
  16. Liu, G. Q., X. P. Jiang, J. Y. Wang and Z. Y. Wang. 2006. Correlations between heterozygosity at microsatellite loci, mean d2 and body weight in a Chinese native chicken. Asian-Aust. J. Anim. Sci. 19(12):1671-1678.
  17. Nei, M. 1973. The theory and estimation of genetic distance in Genetic structure of populations. Honolulu: University of Hawaii Press. United States. pp. 45-54.
  18. Nei, M. 1978. Estimation of average hetorozygosity and genetic distance from a small number of individuals. Genet. 89:583-590.
  19. Nei, M. and S. Kimura. 2000. in Molecular Evolution and Phylogenetics. Oxford: Oxford Univesity Press. England. pp. 235-236.
  20. Nozawa, K., H. Chang and Z. J. Ren. 1996. Effects of domestication to animal genetic variation. J. Husb. Vet. 3:55-56.
  21. Olowofeso, O., J. Y. Wang, P. Zhang, G. J. Dai, H. W. Sheng, R. Wu and X. Wu. 2006. Genetic analysis of Haimen chicken populations using decamer random markers. Asian-Aust. J. Anim. Sci. 19(11):1519-1523.
  22. Osman, S. A. M., M. Sekino, A. Nishihata, Y. Kobayashi, W. Takenaka, K. Kinoshita, T. Kuwayama, M. Nishibori, Y. Yamamoto and M. Tsudzuki. 2006. The genetic variability and relationships of Japanese and foreign chickens assessed by microsatellite DNA profiling. Asian-Aust. J. Anim. Sci. 19(10):1369-1378.
  23. Read, K. M., K. M. Mendoza and C. W. Beattie. 2000. Comparative analysis of microsatellite sites in chicken and turkey. Genome. 43(5):796-802.
  24. Sambrook, J., E. F. Fritch and T. Maniartis. 1998. In Molecular Clone: A laboratory manual (2nd ED). Beijing: Science Press. pp. 483-486.
  25. Sano, A., N. Goto and M. Kimura. 1994. Genetic differentiation within commercial quail populations. Jpn. Poult. Sci. 31:276-286.
  26. Su, Y., C. W. Liu, L. Liu, C. H. Ye, W. Q. Cao, Y. Q. Huang, J. Zheng, D. Y. Cai and O. Olowofeso. 2006. Studies on genetic variance of different Chinese duck populations with random amplified polymorphic DNA analysis. Asian-Aust. J. Anim. Sci. 19(4):475-481.
  27. Tu, Y. J., K. W. Chen, S. J. Zhang, Q. P. Tang, Y. S. Gao and N. Yang. 2006. Genetic diversity of 14 indigenous grey goose breeds in China based on microsatellite markers. Asian-Aust. J. Anim. Sci. 19(1):1-6.
  28. Wright, S. 1978. In: Variability within and among natural populations. Chicago: The Univ. of Chicago Press. United States. pp. 35-49.
  29. Wu, X. S., G. H. Chen, D. Q. Wang, X. Y. Zhang, K. H. Wang, R. Cheng, B. Liu, Q. Xu and Q. L. Zhou. 2004. Analysis of genetic relationship among Chinese native chicken breeds using microsatellites marker. Acta Genetica Sinica. 31(1):43-50.
  30. Zhang, X. Q., X. M. Lu, Y. H. Yang, J. S. Liu, G. F. Yang and X. H. Wu. 1998. Population genetic variability of microsatellite polymorphisms and RAPDs in Chinese chicken breeds in Guangdong. Acta Genetica Sinica. 25(2):112-119.

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