Divergence of Genes Encoding Non-specific Lipid Transfer Proteins in the Poaceae Family

  • Jang, Cheol Seong (Institute of Life Science and Natural Resources, Korea University) ;
  • Jung, Jae Hyeong (Division of Biotechnology, Korea University) ;
  • Yim, Won Cheol (Department of Plant Biotechnology, Dongguk University) ;
  • Lee, Byung-Moo (Department of Plant Biotechnology, Dongguk University) ;
  • Seo, Yong Weon (Division of Biotechnology, Korea University) ;
  • Kim, Wook (Division of Biotechnology, Korea University)
  • Received : 2007.02.09
  • Accepted : 2007.05.25
  • Published : 2007.10.31


The genes encoding non-specific lipid transfer proteins (nsLTPs), members of a small multigene family, show a complex pattern of expressional regulation, suggesting that some diversification may have resulted from changes in their expression after duplication. In this study, the evolution of nsLTP genes within the Poaceae family was characterized via a survey of the pseudogenes and unigenes encoding the nsLTP in rice pseudomolecules and the NCBI unigene database. nsLTP-rich regions were detected in the distal portions of rice chromosomes 11 and 12; these may have resulted from the most recent large segmental duplication in the rice genome. Two independent tandem duplications were shown to occur within the nsLTP-rich regions of rice. The genomic distribution of the nsLTP genes in the rice genome differs from that in wheat. This may be attributed to gene migration, chromosomal rearrangement, and/or differential gene loss. The genomic distribution pattern of nsLTP genes in the Poaceae family points to the existence of some differences among cereal nsLTP genes, all of which diverged from an ancient gene. The unigenes encoding nsLTPs in each cereal species are clustered into five groups. The somewhat different distribution of nsLTP-encoding EST clones between the groups across cereal species imply that independent duplication(s) followed by subfunctionalization (and/or neofunctionalization) of the nsLTP gene family in each species occurred during speciation.


Duplication;Evolution;non-specific Lipid transfer protein (nsLTP);Poaceae


Supported by : Korea Research Foundation


  1. Arondel, V., Vegnolle, C., Cantrel, C., and Kader, J. C. (2000) Lipid transfer proteins are encoded by a small mutligene family in Arabidopsis thaliana. Plant Sci. 157, 1−12
  2. Boutrot, F., Guirao, A., Alary, R., Joudrier, P., and Gautier, M. F. (2005) Wheat non-specific lipid transfer protein genes display a complex pattern of expression in developing seeds. Biochim. Biophs. Acta-Gene Struct. Expression 1730, 114−125
  3. Hu, F. Y., Tao, D. Y., Sacks, E., Fu, B. Y., Li, J., et al. (2003) Convergent evolution of perenniality in rice and sorghum. Proc. Natl. Acad. Sci. USA 100, 4050−4054
  4. Hwang, D. H., Kim, S. T., Kim, S. G., and Kang, K. Y. (2007) Comprehensive analysis of the expression of twenty-seven ${\beta}$- 1, 3-glucanase genes in rice (Oryza sativa L.). Mol. Cells 23, 207−214
  5. Maldonado, A. M., Doerner, P., Dixon, R. A., Lamb, C. J., and Cameron, R. K. (2002) A putative lipid transfer protein involved in systemic resistance signaling in Arabidopsis. Nature 419, 399−403 https://doi.org/10.1038/nature01071
  6. Park, C. J., Shin, R., Park, J. M., Lee, G. J., You, J. S., et al. (2002) Induction of pepper cDNA encoding a lipid transfer protein during the response to tobacco mosaic virus. Plant Mol. Biol. 48, 243−254
  7. Rota, M. L. and Sorrells, M. E. (2004) Comparative DNA sequence analysis of mapped wheat ESTs reveals the complexity of genome relationships between rice and wheat. Funct. Intere. Genomics 4, 34−46
  8. The rice chromosomes 11 and 12 sequencing consortia (2005) The sequence of rice chromosomes 11 and 12, rich in disease resistance genes and recent gene duplications. BMC Biol. 3, 20−38
  9. Paterson, A. H., Bowers, J. E., and Chapman, B. A. (2004) Ancient polyploidization predating divergence of the cereals, and its consequences for comparative genomics. Proc. Natl. Acad. Sci. USA 101, 9903−9908
  10. Blanc, G. and Wolfe, K. H. (2004) Widespread paleoployploidy in model plant species inferred from age distribution of duplicate genes. Plant Cell 16, 1667−1668
  11. Freeling, M. (2001) Grasses as a single genetic system. Reassessment 2001. Plant Physiol. 125, 1191−1197
  12. Jang, C. S., Kim, D. S., Bu, S. Y., Kim, J. B., Lee, S. S., et al. (2002) Isolation and characterization of lipid transfer protein (LTP) genes from a wheat-rye translocation line. Plant Cell Rep. 20, 961−966
  13. Kumar, S., Tamura, K., and Nei, M. (2004) MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform. 17, 1483−1498
  14. Bodt, S. D., Theissen, G., and Van de Peer, Y. (2006) Promoter analysis of MADS-box genes in eudicots through phylogenetic footprinting. Mol. Biol. Evol. 23, 1293−1303
  15. Jang, C. S., Kim, J. Y., Haam, J. W., Lee, M. S., Kim, D. S., et al. (2003) Expression sequence tags from a wheat-rye translocation line (2BS/2RL) infested by larvae of Hessian fly [Mayetiola destructor (Say)]. Plant Cell Rep. 22, 150−158
  16. Bowers, J. E., Chapman, B. A., Rong, J., and Paterson, A. H. (2003) Unravelling angiosperm genome evolution by phylogenetic analysis of chromosomal duplication events. Nature 422, 433−438 https://doi.org/10.1038/nature01506
  17. Jung, H. W., Kim, W., and Hwang, B. K. (2003) Three pathogen- inducible genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, environmental stresses. Plant Cell Environ. 26, 915−928
  18. Tchnag, F., This, P., Stiefel, V., Arondel, V., Morch, M. D., et al. (1998) Phospholipid transfer protein: full-length cDNA and amino acid sequences in maize. Amino acid sequence homologies between plant phospholipid transfer proteins. J. Biol. Chem. 263, 16849− 16856
  19. Gausing, K. (1994) Lipid transfer protein genes specifically expressed in barley leaves and coleoptiles. Planta 192, 574− 580
  20. Jang, C. S., Johnson, J. W., and Seo, Y. W. (2005) Differential expression of TaLTP3 and TaCOMT1 induced by Hessian fly larval infestation in a wheat-rye translocation line possessing H21 resistance gene. Plant Sci. 168, 1319−1326
  21. Molina, A., Diaz, I., Indra, K., Carbonero, P., and García- Olmedo, F. (1996) Two cold-inducible genes encoding lipid transfer protein LTP4 from barley show differential responses to bacterial pathogens. Mol Gen. Genet. 252, 162− 168
  22. Papp, B., Pál, C., and Hurst, L. D. (2003) Evolution of cisregulatory elements in duplicated genes of yeast. Trends Genet. 19, 417−422 https://doi.org/10.1016/S0168-9525(03)00175-6
  23. Jang, C. S., Lee, H. J., Chang, S. J., and Seo, Y. W. (2004) Expression and promoter analysis of the TaLTP1 gene induced by drought and salt stress in wheat (Triticum aestivum L.). Plant Sci. 167, 995−1001
  24. Mena, M., Ambrose, B. A., Meeley, R. B., Briggs, S. P., Yanofsky, M. F., et al. (1996) Diversification of C-function activity in maize flower development. Science 274, 1537−1540
  25. Pelese-Siebenbourg, F., Caelles, C., Kader, J. C., Delseny, M., and Puigomench, P. (1994) A pair of genes coding for lipidtransfer proteins in Sorghum vulgare. Gene 148, 305−308
  26. Vignols, F., Lund, G., Pammi, S., Trèmousaygue, D., Grellet, F., et al. (1994) Characterization of a rice gene encoding for a lipid transfer protein. Gene 142, 265−270
  27. Hall, T. A. (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98NT. Nucl. Acids Symp. Ser. 41, 95−98
  28. Moore, R. C. and Purugganan, M. D. (2005) The evolutionary dynamics of plant duplicate genes. Curr. Opin. Plant Biol. 8, 122−128
  29. Ohno, S. (1970) Evolution by gene duplication. Springer-Verlag
  30. Gu, Z., Rifikin, S. A., White, K. P., and Li, W. H. (2004) Duplicate genes increase gene expression diversity within and between species. Nat. Genet. 36, 577−579 https://doi.org/10.1038/ng1358
  31. Saitou, N. and Nei, M. (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4, 406−425
  32. Torres-Schumann, S., Godoy, J. A., and Pintor-Toro, J. A. (1992) A probable lipid transfer protein gene is induced by NaCl in stems of tomato plants. Plant Mol. Biol. 18, 749−757
  33. Vignols, F., Wigger, M., Garcìa-Garrido, J. M., Grellet, F., Kader, J. C., et al. (1997) Rice lipid transfer protein (LTP) genes belong to a complex multigene family and are differentially regulated. Gene 195, 177−186