DOI QR코드

DOI QR Code

Somatic Cell Nuclear Transfer of Oocytes Aspirated from Postovulatory Ovarian Follicles of Superovulated Rabbits

  • Shang, Jiang-Hua (Department of Animal Physiology, College of Biological Sciences, China Agricultural University) ;
  • Xu, Ru-Xiang (Neuromedical Institute, Zhujiang Hospital, Southern Medical University) ;
  • Jiang, Xiao-Dan (Neuromedical Institute, Zhujiang Hospital, Southern Medical University) ;
  • Zou, Yu-Xi (Neuromedical Institute, Zhujiang Hospital, Southern Medical University) ;
  • Qin, Ling-Sha (Neuromedical Institute, Zhujiang Hospital, Southern Medical University) ;
  • Cai, Ying-Qian (Neuromedical Institute, Zhujiang Hospital, Southern Medical University) ;
  • Yang, Zhi-Jun (Neuromedical Institute, Zhujiang Hospital, Southern Medical University) ;
  • Zheng, Xing (Department of Animal Physiology, College of Biological Sciences, China Agricultural University) ;
  • Cui, Sheng (Department of Animal Physiology, College of Biological Sciences, China Agricultural University)
  • 투고 : 2006.10.09
  • 심사 : 2006.12.17
  • 발행 : 2007.09.01

초록

The aim of this study was to evaluate if oocytes, aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration, could be efficiently used as ooplasm recipients for somatic cell nuclear transfer (SCNT). Within a common SCNT protocol, a comparison between oocytes recovered by direct aspiration (aspirated) from available ovarian follicles and oocytes flushed out from oviducts (flushed) was carried out. The results showed that maturation and enucleation rates of aspirated oocytes were 70.7% and 69.2%, significantly lower than 95.3% (p<0.01) and 83.6% (p<0.05), respectively, from flushed oocytes. However, following enucleation of matured oocytes as ooplasm recipients for SCNT, no difference was recorded in fusion and cleavage rates, as well as blastocyst development from cleaved embryos or hatching of blastocysts between aspirated and flushed groups. Additionally, some matured aspirated and flushed oocytes were also used for immediate parthenogenetic activation and the resulting embryo development was not significantly different. Results from this study show the following: i) the majority of oocytes aspirated from postovulatory ovarian follicles of superovulated rabbits 14 h post-hCG administration are matured and can be used directly as ooplasm recipients for SCNT; ii) the reconstructed embryos derived from these oocytes have similar in vitro developmental ability to those flushed from the oviducts.

키워드

Nuclear Transfer;Oocyte;Rabbit;Follicle;Somatic Cell

과제정보

연구 과제 주관 기관 : Natural Science Foundation of China, Natural Science Foundation for Outstanding Young Scientists of China

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