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Identification of a Novel Human Zinc Finger Gene, ZNF438, with Transcription Inhibition Activity

  • Zhong, Zhaomin (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Wan, Bo (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Qiu, Yun (Anesthesiology Department, Suzhou Yongding Hospital) ;
  • Ni, Jun (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Tang, Wenwen (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Chen, Xinya (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Yang, Yun (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Shen, Suqin (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Wang, Ying (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Bai, Meirong (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Lang, Qingyu (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University) ;
  • Yu, Long (State Key Laboratory of Genetic Engineering, School of Life Science, Fudan University)
  • Published : 2007.07.31

Abstract

There were many different families of zinc finger proteins that contained multiple cysteine and/or histidine residues and used zinc to stabilize their folds. The classical C2H2 zinc finger proteins were the founding members of this superfamily and were among the most abundant proteins in eukaryotic genomes. C2H2 proteins typically contained several C2H2 fingers that made tandem contacts along the DNA. Here we reported a novel C2H2 type zinc finger gene, ZNF438, which encoded 828 amino acids that formed five zinc finger domains. Bioinformatics analysis revealed that the ZNF438 was mapped to human chromosome 10p11.2 and shared 62% identity with rat and mouse homologues. RT-PCR analysis indicated that it was ubiquitously expressed in 18 human adult tissues. With immunofluorescence assay, it was shown that the exogenous Flag-tagged ZNF438 was located in nucleus of COS-7 cells. To further explore the function of ZNF438, we examined the transcriptional activity of ZNF438 protein by transfecting recombinant pM-ZNF438 into mammalian cells. The subsequent analysis based on the duel luciferase assay system showed that ZNF438 was a transcriptional repressor.

Keywords

C2H2;Transcription inhibition;Transcriptional repressor;Zinc finger;ZNF438

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