Effects of Bovine Somatotropin (bST) Administration Combined with Controlled Internal Drug Release (CIDR) on Embryo Quality and Pregnancy of Hanwoo (Korean Native Beef Cattle) during Commercial Embryo Transfer Program

  • Lee, Ho-Jun (The Graduate School of Bio- and Information Technology, Hankyong National University) ;
  • Hwang, Seongsoo (Animal Biotechnology Division, National Livestock Research Institute) ;
  • Yoon, Jong-Taek (Department of Animal Life and Resources, Hankyong National University)
  • Received : 2006.04.04
  • Accepted : 2006.07.17
  • Published : 2007.02.01


Effects of recombinant bovine somatotropin (bST) on plasma hormonal concentration, embryo quality, and pregnancy rate were examined during the superovulation and synchronization treatment in donor and recipient cows. Hanwoos (Korean native beef cattle) were treated with controlled internal drug release (CIDR) combined with bST (CIDR+bST) or without bST (CIDR) as donor cows. The embryos recovered from donors were transferred into Holstein recipient heifers treated with bST (CIDR+bST) or without bST (CIDR) for synchronization. The correlation between IGF-I and P4 showed a positive pattern in the CIDR+bST group (r=0.44, p<0.01), but a negative pattern was shown in the CIDR group (r = -0.59, p<0.02) at day 7 of estrous cycles. Although the number of recovered, transferable, and degenerated embryos was not different, quantities of grade 1 (excellent) embryos in CIDR+bST group were significantly higher than those of the CIDR group (p<0.01). The pregnancy rate was higher in the CIDR+bST recipient group compared to CIDR group (p<0.05), when the embryos were recovered from the donors treated with CIDR. However, the pregnancy was maintained highly in both recipient groups, when the embryos were produced by CIDR+bST treated donors. It can be concluded that bST administration combined with CIDR is an effective method for superovulation and synchronization treatment to stabilize plasma hormonal levels, to obtain excellent quality of embryos, and to get higher pregnancy rate.


Bovine Somatotropin;Embryo Transfer Program;Corpus Luteum;Plasma Hormonal Level;Pregnancy;bST (Bovine Somatotropin);CIDR (Controlled Internal Drug Release);CL (Corpus Luteum);D (Donor);R (Recipient)


  1. Bilby, T. R., A. Guzeloglu, S. Kamimura, S. M. Pancarci, F. Michel, H. H. Head and W. W. Thatche. 2004. Pregnancy and bovine somatotropin in nonlactating dairy cows: I. ovarian, conceptus, and insulin-like growth factor system responses, J. Dairy Sci. 87:3256-3267.
  2. Macmillan, K. L. and C. R. Burke. 1996. Effects of estrous control on reproductive efficiency. Anim. Repro. Sci. 42:307-320.
  3. Moallem, U., M. Kaim, Y. Folman and D. Sklan. 1997. Effect of calcium soaps of fatty acids and administration of somatotropin in early lactation on productive and reproductive performance of high producing dairy cows. J. Dairy Sci. 80:2127-2136.
  4. Thatcher, W. W., F. Moreira, J. E. P. Santos, R. Mattos, C. F. L. Lopes, S. M. Pancaci and C. A. Risco. 2001. Effect of hormone treatments on reproductive performance and embryo production. Theriogenol. 55:75-89.
  5. Veronesi, M. C., G. Gabai, M. Battocchio, A. Mollo, F. Soldano, G. Bono and F. Cairoli. 2002. Ultrasonographic appearance of tissue is a better indicator of CL function than CL diameter measurement in dairy cows. Theriogenol. 58:61-68.
  6. Ryan, D. P., J. A. Galvin and K. J. O'Farrell. 1999. Comparison of oestrous synchronization regimens for lactating dairy cows. Anim. Repro. Sci. 56:153-168.
  7. Kastelic, J. P., D. R. Bergfelt and O. J. Ginther. 1990. Relationship between ultrasonic assessment of the CL and plasma progesterone concentration in heifers. Theriogenol. 33:1269-1278.
  8. Robinson, R. S., G. E. Mann, T. S. Gadd, G. E. Lamming and G. C. Wathes. 2000. The expression of the IGF system in the bovine uterus throughout the oestrous cycle and early pregnancy. J. Endocrinol. 165:231-243.
  9. Lucy, M. C., H. J. Billings, W. R. Butler, L. R. Ehnis, M. J. Fields, D. J. Kesler, J. E. Kinder, R. C. Mattos, R. E. Short, W. W. Thatcher, R. P. Wettemann, J. V. Yelich and H. D. Hafs. 2001. Efficacy of an intravaginal progesterone insert and an injection of $PGF_{2\alpha}$ for synchronizing estrus and shortening the interval to pregnancy in postpartum beef cows, peripubertal beef heifers, and dairy heifers. J. Anim. Sci. 79:982-995.
  10. Staigmiller, R. B., R. A. Bellows, G. B. Anderson, G. E. Seidel, W. D. Foote, A. R. Menino and R. W. Wright. 1992. Superovulation of cattle with equine pituitary extract and porcine FSH. Theriogenol. 37:1091-1099.
  11. Lucy, M. C. 2000. Regulation of ovarian follicular growth by somatotropin and insulin-like growth factors in cattle. J. Dairy Sci. 83:1635-1647.
  12. Marques, M. O., L. F. Nasser, R. P. C. Silva, G. A. Bo and P. S. Baruselli. 2003. Increased pregnancy rates in Bos taurus$\times$Bos indicus embryo recipients with treatments to increase plasma progesterone concentration. Theriogenol. 59:369 (Abstract).
  13. Lafri, M., C. Ponsart, M. Nibart, M. Durand, A. Morel, N. Jeanguyot, F. Badinand, K. De Mari and P. Humblot. 2002. Influence of CIDR treatment during superovulation on embryo production and hormonal patterns in cattle. Theriogenol. 58:1141-1151.
  14. Luna-Dominguez, J. E., R. M. Enns, D. V. Armstrong and R. L. Ax. 2000. Reproductive performance of Holstein cows receiving somatotropin. J. Dairy Sci. 83:1451-1455.
  15. Goulding, D., D. H. Williams, P. Duffy, M. P. Boland and J. F. Roche. 1990. Superovulation in heifers given FSH initiated either at day 2 or day 10 of the estrus cycle. Theriogenol. 34:767-778.
  16. Hasler, J. F., C. R. Bilby, R. J. Collier, S. C. Denham and M. C. Lucy. 2003. Effect of recombinant bovine somatotropin on superovulation response and recipient pregnancy rates in a commercial embryo transfer program. Theriogenol. 59:1919-1928.
  17. Lucy, M. C., C. R. Bilby, C. J. Kirby, W. Yuan and C. K. Boyd. 1999. Role of growth hormone in development and maintenance of follicles and corpora lutea. J. Reprod. Fertil. Suppl. 54:49-59.
  18. Stringfellow, D. A. and S. M. Seidel. 1990. Manual of the International Embryo Transfer Society. Savoy (IL): International Embryo Transfer Society.
  19. Moreira, F., C. Orlandi, C. A. Risco, R. Mattos, F. Lopes and W. W. Thatcher. 2001. Effects of pre-synchronization and bovine somatotropin on pregnancy rates to a timed artificial insemination protocol in lactating dairy cows. J. Dairy Sci. 84:1646-1659.
  20. Spell, A. R., W. E. Beal, L. R. Corah and G. C. Lamb. 2001. Evaluating recipient and embryo factors that affect pregnancy rates of embryo transfer in beef cattle. Theriogenol. 56:287-297.
  21. Davis, T. L., M. L. Mussard, H. Jimenez-Severiano, W. J. Enright and J. Kinder. 2003. Chronic treatment with an agonist of gonadotropin-releasing hormone enhances luteal function in cattle. Biol. Reprod. 69:398-403.
  22. Morales-Roura, J. S., L. Zarco, J. Hernandez-Ceron and G. Rodriguez. 2001. Effect of short term treatment with bovine somatotropin at estrus on conception rate and luteal function of repeat breeding dairy cows. Theriogenol. 55:1831-1841.
  23. Xu, Z. Z., G. A. Verkerk, J. F. Mee, S. R. Morgan, B. A. Clark, C. R. Burke and L. J. Burton. 2000. Progesterone and follicular changes in postpartum noncyclic dairy cows after treatment with progesterone and estradiol or with progesterone, GnRH, PGF2$\alpha$, and estradiol. Theriogenol. 54:273-282.
  24. Moreira, F., L. Badinga, C. Burnley and W. W. Thatcher. 2002. Bovine somatotropin increases embryonic development in superovulated cows and improves post-transfer pregnancy rates when given to lactating recipient cows. Theriogenol. 57:1371-1387.
  25. Zi, X. D., S. Chang, H. Lu and X. Wang. 2005. Estrous response and fertility in postpartum suckled female yaks (Bos grunniens) treated with an intravaginal device containing progesterone (CIDR), pregnant mares' serum gonadotropin and prostaglandin analogue. Asian-Aust. J. Anim. Sci. 18:470-474.
  26. Chenault, J. R., J. F. Boucher, K. J. Dame, J. A. Meyer and S. L. Wood-Follis. 2003b. Intravaginal progesterone insert to synchronize return to estrus of previously inseminated dairy cows. J. Dairy Sci. 86:2039-2049.
  27. Xu, Z. Z., L. J. Burton and K. L. Macmillan. 1996. Reproductive performance of lactating dairy cows following oestrus synchronization with progesterone, oestradiol and prostaglandin. N.Z. Vet. J. 44:99-104.
  28. Nasser, L. F., E. L. Reis, M. A. Oliveira, G. A. Bo and P. S. Baruselli. 2004. Comparison of for synchronization protocols for fixed-time bovine embryo transfer in Bos indicus$\times$Bos taurus recipients. Theriogenol. 62:1577-1584.
  29. Shea, B. F., R. E. Janzen and D. P. McDermand. 1984. Seasonal variation and related embryo transfer procedures in Alberta over a nine year period. Theriogenol. 21:186-195.
  30. Woad, K. J., G. Baxter, C. O. Hogg, T. A. Bramley, R. Webb and D. G. Armstrong. 2000. Expression of mRNA encoding insulinlike growth factors I and II and the type 1 IGF receptor in the bovine corpus luteum at defined stages of the oestrous cycle. J. Reprod. Fertil. 120:293-302.
  31. Lucy, M. C., W. W. Thatcher, R. J. Collier, F. A. Simmen, Y. Ko, J. D. Savio and L. Badinga. 1995. Effects of somatotropin on the conceptus, uterus, and ovary during maternal recognition of pregnancy in cattle. Domest. Anim. Endocrinol. 12:73-82.
  32. Alnimer, M. and W. Lubbadeh. 2003. Effect of using progesterone releasing intravaginal device with ovsynch program on reproduction in dairy cattle during summer season. Asian-Aust. J. Anim. Sci. 16:1268-1273.
  33. Bo, G. A., D. K. Hockley, L. F. Nasser and R. J. Mapletoft. 1994. Superovulatory response to a single subcutaneous injection of Folltropin-V in beef cattle. Theriogenol. 42:963-975.
  34. Sartori, R., C. A. Suarez-Fernandez, R. L. Monson, J. N. Guenther, G. J. M. Rosa and M. C. Wiltbank. 2003. Improvement in recovery of embryos/ova using a shallow uterine horn flushing technique in superovulated Holstein heifers. Theriogenol. 60:1319-1330.
  35. Cushman, R. A., J. C. DeSouza, V. S. Hedgpeth and J. H. Britt. 2001. Effect of long-term treatment with recombinant bovine somatotropin and estradiol on hormone concentrations and ovulatory response of superovulated cattle. Theriogenol. 55:1533-1547.
  36. Santos, J. E., S. O. Juchem, R. L. Cerri, K. N. Galvao, R. C. Chebel, W. W. Thatcher, C. S. Dei and F. R. Bilby. 2004. Effect of bST and reproductive management on reproductive performance of Holstein dairy cows. J. Dairy Sci. 87:868-881.
  37. Chenault, J. R., R. E. Hornish, Y. C. Anderson, L. F. Krabill, J. F. Boucher and M. J. Prough. 2003a. Concentrations of progesterone in milk of cows administered an intravaginal progesterone insert. J. Dairy Sci. 86:2050-2060.