In vitro Methanogenesis and Fermentation of Feeds Containing Oil Seed Cakes with Rumen Liquor of Buffalo

  • Kumar, Ravindra (College of Veterinary Science, Shere-Kashmir University of Agriculture Science and Technology) ;
  • Kamra, D.N. (Rumen Microbiology Laboratory, Centre of Advanced Studies in Animal Nutrition Indian Veterinary Research Institute) ;
  • Agarwal, Neeta (Rumen Microbiology Laboratory, Centre of Advanced Studies in Animal Nutrition Indian Veterinary Research Institute) ;
  • Chaudhary, L.C. (Rumen Microbiology Laboratory, Centre of Advanced Studies in Animal Nutrition Indian Veterinary Research Institute)
  • Received : 2006.05.19
  • Accepted : 2006.10.18
  • Published : 2007.08.01


Eight feeds (mixture of wheat straw and oil seed cakes in 3:1 ratio) were evaluated for methane emission and fermentation pattern with buffalo rumen liquor as inoculum in an in vitro gas production test. The cakes tested were groundnut cake (GNC), soybean cake (SBC), mustard seed cake (MSC), cotton seed cake (CSC), karanj seed cake expeller extracted (KCEE), karanj seed cake solvent extracted (KCSE), caster bean cake expeller extracted (CBCEE) and caster bean cake solvent extracted (CBCSE). The gas production (ml/g dry matter) was significantly higher with SBC and MSC followed by CSC, GNC, KCSE, KCEE, CBCSE and was the lowest with CBCEE. Methane emission was significantly lower with KCEE, KCSE, CBCEE, CBCSE (20.32- 22.43 ml/g DM) than that with SBC, GNC, CSC (27.34-31.14 ml/g DM). Mustard seed cake was in-between the two groups of oil cakes in methane production. In vitro true digestibility was highest with SBC followed by GNC, CSC, MSC, KCSE, KCEE, CBCSE and CECEE. Ammonia nitrogen level was positively correlated with the amount of protein present in the cake. Total holotrich protozoa were significantly higher with SBC, whereas, large spirotrich protozoa tended to be lower than with other cakes. The counts of small spirotrich and total protozoa were similar with all the cakes. Total volatile fatty acid production and acetate to propionate ratio were significantly higher with SBC and significantly lower with KCEE as compared to the other cakes. Among the conventional oil cakes tested in the present experiment (GNC, SBC, MSC and CSC), mustard seed cake-based feed produced the minimum methane without affecting other fermentation characteristics adversely.


  1. AOAC. 1995. Official method of analysis, 16th edition. Association of Official Analytical Chemists, Washington, DC.
  2. Cottyn, B. G. and C. V. Boucque. 1968. Rapid method for the gas chromatographic determination of volatile fatty acids in rumen fluid. J. Agric. Food Chem. 16:105-107.
  3. Crutzen, P. J. 1995. The role of methane in atmospheric chemistry and climate In: Ruminant physiology: digestion, metabolism, growth and reproduction. (Ed. W. V. Engelhardt et al.) Ferdinand Erke Verlag, pp. 291-314.
  4. Dohme, F., A. Machmüller, A. Wasserfallen and M. Kreuzer. 2001. Ruminal methanogenesis as influenced by individual fatty acids supplemented to complete ruminant diets. Letters Appl. Microbiol. 32:47.
  5. Agarwal, N., D. N. Kamra, L. C. Chaudhary and A. K. Patra. 2006. Effect of Sapindus mukorossi extracts on in vitro methanogenesis and fermentation characteristics in buffalo rumen liquor. J. Appl. Anim. Res. 30:1-4.
  6. Lila, Z. A., N. Mohammed, S. Kanda, M. Kurihara and H. Itabashi. 2005. Sarsaponin effects on ruminal fermentation and microbes, methane production, digestibility and blood metabolites in steers. Asian-Aust. J. Anim. Sci. 18:1746-1751.
  7. Menke, K. H. and H. Steingass. 1988. Estimation of the energetic feed value obtained by chemical analysis and in vitro gas production using rumen fluid. Anim. Res. Develop. 28:7-55.
  8. Nagaraja, T. G., C. J. Newbold, C. J. Van Nevel and D. I. Demeyer. 1997. Manipulation of ruminal fermentation. In: Ruminal Microbial Ecosystem (Ed. P. N. Hobson and C. S. Stewart). Blackie Academic & Professional, London. pp. 523-632.
  9. Patra, A. K., D. N. Kamra and Neeta Agarwal. 2005. Effect of spices on rumen fermentation, methanogenesis and protozoa counts in in vitro gas production test. 2nd International Conference on Greenhouse Gases and Animal Agriculture, Zurich, Switzerland, September 20-24, pp. 115-118.
  10. Patra, A. K., D. N. Kamra and N. Agarwal. 2006. Effect of plant extracts on in vitro methanogenesis, enzyme activities and fermentation of feed in rumen liquor of buffalo. Anim. Feed Sci. Technol. 128(3-4):276-191.
  11. SPSS. 1996. Statistical Packages for Social Sciences version 7.5. SPSS Inc., IL, USA.
  12. Tyler, S. C. 1991. The global methane budget. In microbial production and consumption of green house gases: methane, nitrogen oxide, and halomethane (Ed. J. E. Roger and W. B. Whiteman). Amer. Society Microbiol. Washington, DC. US. pp. 7-38.
  13. Van Soest, P. J. and J. B. Robertson. 1988. A laboratory manual for animal science 612, Cornell University, USA.
  14. Van Soest, P. J., J. B. Robertson and B. A. Lewis. 1991. Methods for fiber, neutral detergent fiber, and non-starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74:3583-3597.
  15. Weatherburn, M. W. 1967. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39:971-974.
  16. Hosoda, K., T. Nishida, W.-Y. Park and B. Eruden. 2005. Influence of Mentha piperita L. (Peppermint) supplementation on nutrient digestibility and energy metabolism in lactating dairy cows. Asian-Aust. J. Anim. Sci. 18:1721-1726.
  17. Lee, H. J., S. C. Lee, J. D. Kim, Y. G. Oh, B. K. Kim, C. W. Kim and K. J. Kim. 2003. Methane production potential of feed ingredients as measured by in vitro gas test. Asian-Aust. J. Anim. Sci. 16:1143-1150.
  18. Fievez, V., F. Piattoni, F. Mbanzamihigo and D. Demeyer. 1999. Reductive acetogenesis in the hindgut and attempts to its induction-a review. J. Appl. Anim. Res. 16:1-22.
  19. Kamra, D. N., R. K. Sawal, N. N. Pathak, N. Kewalramani and N. Agarwal. 1991. Diurnal variations in ciliate protozoa in the rumen of blackbuck (Antilope cervicapra) fed green forages. Lett. Appl. Microbiol. 13:165-167.

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