- Volume 20 Issue 11
DOI QR Code
Effect of Spinal Cord Removal before or after Splitting and Washing on CNST Decontamination of Beef Carcasses
- Lim, D.G. (Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University) ;
- Kim, D.H. (National Institute of Animal Science, R.D.A.) ;
- Lee, M. (Department of Agricultural Biotechnology, College of Agriculture and Life Science, Seoul National University)
- 투고 : 2007.03.22
- 심사 : 2007.06.11
- 발행 : 2007.11.01
Beef carcasses were examined to explore the effects of spinal cord removal and washing on central nervous system tissue (CNST) decontamination of the surface during the slaughtering process. A total of 15 carcasses were split by sawing centrally down the vertebral column and left sides of split carcasses were used for analysis. Samples were collected by swabbing the surface from 4 defined parts on the interior and 4 on the exterior of carcasses from the abattoir and analyzed using an ELISA-based test. The results showed that automatic and manual spray washing decreased CNST contamination, especially on the interior ventral parts of carcass surfaces (p<0.01), but did not decrease CNST on the interior dorsal parts. Increasing washing time to 60 s did not affect the reduction of CNST contamination. Samples following spinal cord removal prior to splitting showed lower calculated levels of "risk material" than the stated limit of detection (0.1%) of the ELISA kit on interior and exterior carcass parts (p<0.01). Therefore, spinal cord removal prior to splitting could be a very effective way to minimize CNST contamination of beef carcasses.
- SAS. 1997. Applied statistics and the SAS programming language. Cary, NC, USA: SAS Institute INC.
- Schmidt, G. R., K. L. Hossner, Yemm and D. H. Gould. 1999. Potential for disruption of central nervous system tissue in beef cattle by different types of captive bolt stunners. J. Food Prot. 62:390-393. https://doi.org/10.4315/0362-028X-62.4.390
- Schmidt, G. R., R. S. Yemm, K. D. Childs, J. P. O'callaghan and K. L. Hossner. 2001. The detection of central nervous system tissue on beef carcasses and in comminuted beef. J. Food Prot. 64:2047-2052. https://doi.org/10.4315/0362-028X-64.12.2047
- Schwagele, G., M. Moje, K. Troeger and K. O. Hoinkel. 2002. Detection of central nervous system (CNS) tissue on beef carcasses after sucking off the spinal cord tissue and splitting. Proceedings of the 48th International Congress of Meat Science and Technology. Rome, Italy, Aug 25 to 30, 2002, pp. 958-959.
- Smith, G. C. 2005. Removal and handling of BSE specified risk material, in: Improving the safety of fresh meat (Ed. J. N. Sofos and F. Boca Raton). CRC Press, pp. 273-302.
- Sheridan, J. J. 1998. Sources of contamination during slaughter and measures for control. In food safety: the implications of change from producerism to consumerism (Ed. J. J. Sheridan, M. O'Keeffe, M. Rogers). Trumbull, Food & Nutrition Press. pp. 137-155.
- Prendergast, D. M., J. J. Sheridan, D. J. Daly, D. A. McDowell and I. S. Blair. 2003. Dissemination of central nervous system tissue from the brain and spinal cord of cattle after captive bolt stunning and carcass splitting. Meat Sci. 65:1201-1209. https://doi.org/10.1016/S0309-1740(03)00026-3
- Helps, C. R., A. V. Fisher, D. H. Harbour, D. H. O'Neill and A. C. Knight. 2004. Transfer of spinal cord material to subsequent bovine carcass at splitting. J. Food Prot. 67:1921-1926. https://doi.org/10.4315/0362-028X-67.9.1921
- Lim, D. G., Y. Erwanto and M. Lee. 2007. Comparison of stunning methods in the dissemination of central nervous system tissue on the beef carcass surface. Meat Sci. 75:622-627. https://doi.org/10.1016/j.meatsci.2006.09.011
- Lopes, M., J. D. Stopforth, K. Sucre, R. R. Miksch, E. Giddens, M. C. S. Reddy, R. S. Yemm and M. Samadpour. 2006. Alternative cutting methods to minimize transfer of nervous system tissue during steak preparation form bone-in short loins. J. Food Prot. 69:1388-1392. https://doi.org/10.4315/0362-028X-69.6.1388
- Garland, T., N. Bauer and M. Bailey. 1996. Brain emboli in the lungs of cattle after stunning. Lancet 348:610-611.
- Hajmeer, M. N., O. Cliver and J. L. Marsden. 2006. Central nervous system tissue detection in meat from advanced meat recovery systems. Meat Sci. 72:656-659. https://doi.org/10.1016/j.meatsci.2005.09.015
- Helps, C. R., P. Hindel, T. J. Hillman, A. V. Fisher, H. Anil, A. C. Knight, R. T. Whyte, D. H. O`Niell, T. G. Knowles and D. A. Harbour. 2002. Contamination of beef carcasses by spinal cord tissue during splitting. Food Cont. 13:417-423. https://doi.org/10.1016/S0956-7135(01)00040-8
- Doherty, M., J. M. Mcevoy, J. J. Sheridan, L. Mcguire and M. O'Sullivan. 1999. Development of HACCP analysis systems for beef slaughter. Research report No. 5. Dublin, The National Food Center, Teagasc. pp. 1-16.
- European Commission. 1997. Prohibition of use of risk materials (TSE). Commission Decision 97/534/EC, off. J. Eur. Communities, L216/95.
- Frederick, T., L. Detwiler, G. Bellinger, S. Wood, W. Warren-Serna, H. R. Cross and G. C. Smith. 2004. Best practices for removal and disposition of SRM, final report to packerland packing company (Green bay, WI), San Antonio, TX, Food safety net services, Ltd, 1-7.
- Anil, M. H., S. Love, C. R. Helps and D. A. Harbour. 2002. Potential for carcasses contamination with brain tissue following stunning and slaughter in cattle and sheep. Food Contl. 13:431-436. https://doi.org/10.1016/S0956-7135(01)00055-X
- Cabedo, L., J. N. Sofos and G. C. Smith. 1996. Removal of bacteria from beef tissue by spray washing after different times of exposure to fecal material. J. Food Prot. 59:1284-1287. https://doi.org/10.4315/0362-028X-59.12.1284
- Rotterud, O. J., C. R. Helps, T. J. Hillman, A. V. Fisher, D. Harbour, H. Anil and T. Nesbakken. 2006. Hot boning of intact carcasses: a procedure to avoid central nervous system selfcontamination in beef and beef products. J. Food Prot. 69:405-411. https://doi.org/10.4315/0362-028X-69.2.405
- Lucker, E., B. Schlottermuller and A. Martin. 2002. Studies on contamination of beef with tissues of the CNS as pertaining to slaughter technology and human BSE-exposure risk. Berl Munch Tierarztl Wochenschr. 115:118-121.
- Troeger, K. 2004. Overview of current and alternative slaughter practices. Biotechnol. Agron. Soc. Environ. 8(4):275-281.
- Prendergast, D. M., J. J. Sheridan, D. J. Daly, D. A. McDowell and I. S. Blair. 2004. Dissemination of central nervous system tissue from the brain and spinal cord of cattle after captive bolt stunning and carcasses splitting. Meat Sci. 65:1201-1209. https://doi.org/10.1016/S0309-1740(03)00026-3
- Choi, S. H., S. H. Chae, H. H. Choi, J. S. Kim, B. R. Kang, J. S. Yeo, I. Choi, Y. S. Lee, Y. H. Choy and H. S. Park. 2007. Genomic sequence variability of the prion gene (PRNP) in Korean cattle. Asian-Aust. J. Anim. Sci. 20:653-660. https://doi.org/10.5713/ajas.2007.653
- Quantitative Analysis of Central Nervous System Tissues (CNST) in Beef By-Products in Retail Market vol.32, pp.2, 2012, https://doi.org/10.5851/kosfa.2012.32.2.247