Microwave fixation of Setaria Digitata for scanning electron microscopy

선충류의 주사전자현미경적 관찰을 위한 마이크로웨이브 고정법

  • Lee, San-Soo (Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University) ;
  • Cho, Kyoung-Oh (Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University) ;
  • Shin, Kil-Sang (College of Natural Sciences, Soonchunhyang University) ;
  • Shin, Sung-Shik (Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University)
  • Accepted : 2007.05.14
  • Published : 2007.06.30


Conventional processing of biological materials including nematode parasites for scanning electron microscopy includes fixation with glutaraldehyde and osmium, followed by dehydration in an ascending grade of ethanol, and finally freeze drying. This procedure takes about 8 to 12 h depending on the characteristics of samples. Microwave irradiation of 2,450 MHz enhance the action of cross-linking fixatives and can greatly accelerate various stages of tissue processing. In this study, samples of nematode parasites, Setaria digitata, were fixed by a combination of conventional chemical fixation and the microwave irradiation during the process. The microwave irradiation was also incorporated in the serial dehydration process with ethanol. The complete procedure from the initial fixation to the completion of dehydration with ethanol was reduced to 1 h with good preservation of the ultrastructural details of the specimens.


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