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Intramuscular Administration of Zinc Metallothionein to Preslaughter Stressed Pigs Improves Anti-oxidative Status and Pork Quality

  • Li, L.L. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Hou, Z.P. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Yin, Y.L. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Liu, Y.H. (Laboratory of Animal Nutrition and Health and Key Laboratory of subtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Hou, D.X. (Department of Biochemical Technology, Faculty of Agriculture, Kagoshima University) ;
  • Zhang, B. (College of Animal Science and Technology, Hunan Agricultural University) ;
  • Wu, G.Y. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Kim, S.W. (Department of Animal and Food Sciences, Texas Tech University) ;
  • Fan, M.Z. (Department of Animal and Poultry Science, University of Guelph) ;
  • Yang, C.B. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Kong, X.F. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences,) ;
  • Tang, Z.R. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Peng, H.Z. (College of Animal Science and Technology, Hunan Agricultural University) ;
  • Deng, D. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Deng, Z.Y. (The key Laboratory of Food Science of Ministry of Education, Nanchang University) ;
  • Xie, M.Y. (The key Laboratory of Food Science of Ministry of Education, Nanchang University) ;
  • Xiong, H. (The key Laboratory of Food Science of Ministry of Education, Nanchang University) ;
  • Kang, P. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences) ;
  • Wang, S.X. (Laboratory of Animal Nutrition and Health and Key Laboratory of ubtropical Agro-ecology Institute of Subtropical Agriculture, The Chinese Academy of Sciences)
  • Received : 2006.07.02
  • Accepted : 2006.09.05
  • Published : 2007.05.01

Abstract

This study was conducted to determine the effects of exogenous zinc-metallothionein (Zn-MT) on anti-oxidative function and pork quality. After feeding a corn-soybean meal-based diet for two weeks, 48 pigs ($Duroc{\times}Landrace{\times}Chinese\;Black Pig$) were assigned randomly to four groups. Pigs in Group 1 were maintained under non-stress conditions, whereas pigs in Groups 2, 3 and 4 were aggressively handled for 25 min to produce stress. Pigs in Groups 1, 2, 3, and 4 received intramuscular administration of saline (control group; CON), 0 (negative control group; NCON), 0.8 (low dose group; LOW), and 1.6 (high dose group; HIGH) mg rabbit liver Zn-MT per kg body weight, respectively. Pigs were slaughtered at 3 and 6 h post-injection. Zn-MT treatment increased (p<0.05) the activities of superoxide dismutase (SOD) and glutathione-peroxidase (GSH-PX) while decreasing the concentration of malondialdehyde (MDA) in liver. These responses were greater (p<0.05) at 6 h than at 3 h post Zn-MT injection. Zn-MT treatment increased (p<0.05) hepatic SOD mRNA levels in a time and dose-dependent manner and decreased (p<0.05) serum glutamate-pyruvate transaminase and lactate dehydrogenase activities (indicators of tissue integrity). Zn-MT administration decreased (p<0.05) lactate concentration and increased (p<0.05) pH and water-holding capacity in the longissimus thorasis meat. Collectively, our results indicate that intramuscular administration of Zn-MT to pre-slaughter stressed pigs improved tissue anti-oxidative ability and meat quality.

Keywords

Stress;Metallothionein;Anti-oxidative Enzyme;Superoxide Dismutase Gene Expression;Pork Meat Quality

Acknowledgement

Supported by : National Science Foundation of China

References

  1. Cai, L., J. B. Klein and Y. J. Kang. 2000. Metallothionein inhibits peroxynitrite-induced DNA and lipoprotei damage. J. Biol. Chem. 275:38957-38960. https://doi.org/10.1074/jbc.C000593200
  2. Chen, Q. M. and L. X. Wang. 1997. Modern Pig Production. China Agricultural University Press.
  3. D'Souza, D. N., F. R.Dunshea, R. D. Warner and B. J. Leury. 1998. The effect of handingpre-slaughter and carcass processing rate post-slaughter on pork quality. Meat Sci. 50:429-437. https://doi.org/10.1016/S0309-1740(98)00055-2
  4. Fang, Y. Z., S. Yang and G. Wu. 2002. Free radicals, antioxidants, and nutrition. Nutrition 18:872-879. https://doi.org/10.1016/S0899-9007(02)00916-4
  5. Jin, H. Y., F. Q. Li and D. X. Fang. 2001. Study on the relationship of metallothionein and antioxidases in protective mechanism of cardiomyocytes. Chin. J. Appl. Physiol. 17:61-63.
  6. NPPC. 1994. Pork chain quality audit. Progress report. National Pork Producers Council. Des Moines, IA.
  7. Sato, M. and Z. Bremner. 1993. Oxygen free radicals and metallothionein. Free Radic. Biol. Med. 14:325-337. https://doi.org/10.1016/0891-5849(93)90029-T
  8. Tamai, K. T., E. B. Gralla, L. M. Ellerby, J. S. Valentine and D. J. Thiele. 1993. Yeast and mammalian metallothioneins functionally substitute for yeast copper-zinc superoxide dismutase. Proc. Natl. Acad. Sci. USA. 90:8013-8017. https://doi.org/10.1073/pnas.90.17.8013
  9. Thornalley, P. J. and M. Vasak. 1985. Possible role for metallothionein in protection against radiation- induced oxidative stress kinetics and mechanism of its reaction with superoxide and hydroxyl radicals. Biochim. Biophys. Acta, 827:36-44. https://doi.org/10.1016/0167-4838(85)90098-6
  10. Yang, C. B., A. K. Li, Y. L. Yin, R. L. Huang, T. J. Li, L. L. Li, Y. P. Liao, Z. Y. Deng, J. Zhang, B. Wang, Y. G. Zhang, X. J. Yang, J. Peng and M. Z. Fan. 2005. Effects of dietary supplementation of cysteamine on growth performance, carcass quality, serum hormones and gastric ulcer in finishing pigs. J. Sci. Food Agric. 85:1947-1952. https://doi.org/10.1002/jsfa.2123
  11. Zhang, J., H. Li and B. Ru. 1999. Studies on the function of metallothionein to scavenge hydroxyl radical. ACTA Scientiarum Naturalium Universitatis Pekinensis. 35:573-576.
  12. Zhou, Z. B., H. Q. Ding, F. J. Qin, L. Liu and S. Cheng. 2003. Effect of Zn-metallothionein on oxidative stress in liver of rats with severe thermal injury. Acta Pharmacologica Sinica. 24:764-770.
  13. Yang, Y. 1989. The mechanism and meaning of oxygen free radical produced by lick-up cells. Physiol. Sci. (Chinese). 9:10-13.
  14. Yin, Y. L., S. K. Baidoo, J. L. Boychuk and H. H. Simmins. 2001. Performance and carcass characteristics of growing pigs and broilers fed diets containing micronised barley, ground barley, wheat and maize. J. Sci. Food Agric. 81:1487-1497. https://doi.org/10.1002/jsfa.964
  15. Zhou, X. W. 2000. Animal Biochemistry. China Agricultural Press. Beijing, China.
  16. Wu, G., Y. Z. Fang, S. Yang, J. R. Lupton and N. D. Turner. 2004. Glutathione metabolism and its implications for health. J. Nutr. 134:489-492. https://doi.org/10.1093/jn/134.3.489
  17. Adel, J. and N. de Ruiter. 1989. Inhibition of hydroxyl radicalgenerated DNA degradation by metallothionein. Toxicol. Lett. 47:191-196. https://doi.org/10.1016/0378-4274(89)90075-1
  18. Green, E. M. and H. R. Adams. 1992. New perspectives in circulatory shock: pathophysiologic mediator of the mammalian response to endotoxemia and sepsis. Am. J. Vet. Med. 200:1834-1841.
  19. Rosenvold, K. and H. J. Andersen. 2003. Factors of significance for pork quality-a review. Meat Sci. 64:219-237. https://doi.org/10.1016/S0309-1740(02)00186-9
  20. Sato, M. and M. Sasaki. 1991. Enhanced lipid peroxidation is not necessary for induction of metallothionein-I by oxidative stress. Chem. Biol. Interact. 78:143-154. https://doi.org/10.1016/0009-2797(91)90010-5
  21. Kil, D. Y., S. N. Ryu, L. G. Piao, C. S. Kong, S. J. Han and Y. Y. Kim. 2006. Effect of feeding cyanidin 3-glucoside (C3G) high black rice bran on nutrient digestibility, blood measurements, growth performance and pork quality of pigs. Asian-Aust. J. Anim. Sci. 19:1790-1783. https://doi.org/10.5713/ajas.2006.1790
  22. Yang, J. Z., B. P. Zhao, C. Y. Deng and Y. Z. Xiang. 1993. Pig plasma enzyme activities of glycolysis in relation to halothane genotype and meat quality. J. Huanzhong Agric. Univ. 12:478-481.
  23. Kojima, Y. 1991. Definitions and nomenclature of metallothioneins. In: Methods in Enzymology (Ed. J. F. Riordan and B. L. Vallee). pp. 8-10.
  24. Zhang, J. X. and S. L. Fu. 2003. The relationship between metallothionein and oxidative stress induced by lead in liver of mice offspring. Chin. Occupational Med. 30:11-13.
  25. Bremner, I. 1987. Interactions between metallothiothionein and trace metals. Prog. Food Nutr. Sci. 11:1-37.
  26. SAS. 2000. SAS User's Guide. Cary, NC: SAS Institute, Inc
  27. Tang, Z. R., Y. L. Yin, C. M. Nyachoti, R. L. Huang, T. J. Li, C. B. Yang, X. J. Yang, J. S. Gong, J. Peng, D. S. Qi, J. J. Xing, Z. H. Sun and M. Z. Fan. 2005. Effect of dietary supplementation of chitosan and galacto-mannan-oligosaccharide on serum parameters and the insulin-like growth factor-I mRNA expression in early-weaned piglets. 28:430-441. https://doi.org/10.1016/j.domaniend.2005.02.003
  28. Qi, K., X. Zhang, Q. Gao, C. Wang, X. Zhang and X. Shi. 1999. The study on metabolization of oxygen free radical about endotoxic microcycle in goat. Chin. Agric. Sci. 32:90-95.
  29. Li, Q. P., X. H. Qiao and X. D. Wang. 2003. Effect of dietary supplementation with vitamin E on pork meat quality. J. Chin. Anim. Sci. 39:34-35.
  30. NRC. 1998. Nutrient Requirements of Swine, 10th Edition. National Academy Press, Washington, DC.
  31. Yang, J. Z., Y. Z. Xiong and H. Wang. 1990. Study on serum enzymes and halothan sensitivity in relation to porcine muscle quality. Scientia Agric. Sinica. 23:67-76.

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