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Genomic Sequence Variability of the Prion Gene (PRNP) in Korean Cattle

  • Choi, Sang-Haeng (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chae, Sung-Hwa (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Han-Ho (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Jeong-Seon (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Bo-Ra (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yeo, Jung-Sou (Department of Biotechnology and School of Biotechnology, Yeungnam University) ;
  • Choi, Inho (Department of Biotechnology and School of Biotechnology, Yeungnam University) ;
  • Lee, Yong-Seok (Department of Parasitology and Malariology, PICR, College of Medicine and Frontier Inje Research for Science and Technology, Inje University) ;
  • Choy, Yun-Ho (Hanwoo Experiment Station, Rural Development Administration) ;
  • Park, Hong-Seog (Genome Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2006.05.10
  • Accepted : 2006.08.22
  • Published : 2007.05.01

Abstract

In this study, we have investigated sequence variants in the PRNP gene of 20 individuals belonging to the Korean cattle, and have analyzed and compared genetic features between varieties of other cattle breeds. Of the 73 sequence variants identified in Korean cattle, 27 were identified for the first time in this study, whereas 46 of these polymorphisms had previously been isolated. We discovered a 2.6 kb SNP hot spot region localized on the putative promoter region of the PRNP gene. Furthermore, the copy numbers of the octapeptide repeat (24 bp indel) which is detected on the coding sequence (CDS) of the PRNP exhibited a completely homozygous 6/6 genotype which is dominant in other cattle breeds. We also characterized a new 19 bp/10 bp allele located on the putative promoter region of the PRNP gene, which represented 0.71 in allele frequency. To the best of our knowledge, this report is the first to address polymorphisms of the PRNP gene structure in Korean cattle in which BSE has yet to be discovered. Therefore, our findings may prove useful with regard to our current understanding of allelic diversity in bovine species, and may also provide new insights into the genetic factors associated with susceptibility or resistance to BSE.

Keywords

PRNP;SNP;BSE;Indel;Korean Cattle

Acknowledgement

Supported by : Rural Development Administration

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