Association between PCR-RFLP Polymorphisms of Five Gene Loci and Milk Traits in Chinese Holstein

  • Zhang, R.F. (College of Animal Science and Technology, Northwest A&F University Shaanxi Key Laboratory of Molecular Biology for Agriculture) ;
  • Chen, H. (College of Animal Science and Technology, Northwest A&F University Shaanxi Key Laboratory of Molecular Biology for Agriculture) ;
  • Lei, C.Z. (College of Animal Science and Technology, Northwest A&F University Shaanxi Key Laboratory of Molecular Biology for Agriculture) ;
  • Fang, X.T. (Institute of Cellular and Molecular Biology, Xuzhou Normal University) ;
  • Zhang, Y.D. (College of Animal Science and Technology, Northwest A&F University Shaanxi Key Laboratory of Molecular Biology for Agriculture) ;
  • Hu, S.R. (College of Animal Science and Technology, Northwest A&F University Shaanxi Key Laboratory of Molecular Biology for Agriculture) ;
  • Su, L.H. (College of Animal Science and Technology, Northwest A&F University Shaanxi Key Laboratory of Molecular Biology for Agriculture)
  • Received : 2006.03.21
  • Accepted : 2006.08.22
  • Published : 2007.02.01


The objective of this study was to assess the association of polymorphisms in ${\kappa}$-cn, ${\beta}$-lg, ${\beta}$-lg 5′ flanking region, CSN1S2, and IGFBP-3 genes with milk production traits and mastitis-related traits in Chinese Holstein. Traits analyzed were 305 day standard milk yield, protein percentage, fat percentage, the ratio of fat percentage and protein percentage, pre-somatic cell count, somatic cell count, and somatic cell score, respectively. CSN1S2 locus was uninformative because only one genotype BB was found in Chinese Holstein. Allele frequencies of A and B in IGFBP-3 gene were 0.5738 and 0.4262 in Chinese Holstein population, which was different from reported Qinchuan cattle population. The genotypes of animals at IGFBP-3 locus significantly affected 305 day standard milk yield, protein percentage, and somatic cell score. The ${\beta}$-lg genotypes had a significant effect on protein percentage and the ratio of fat percentage and protein percentage. Polymorphism in ${\beta}$-lg 5′ flanking region was associated with 305 day standard milk yield, protein percentage, fat percentage, pre-somatic cell count, and somatic cell count. No significant associations of the polymorphism in ${\kappa}$-cn gene were observed for any trait.


Chinese Holstein;${\beta}$-lg;${\kappa}$-cn;IGFBP-3;Milk Traits


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