DOI QR코드

DOI QR Code

The Use of Fungal Inoculants in the Ensiling of Potato Pulp: Effect of Temperature and Duration of Storage on Silage Fermentation Characteristics

  • Okine, A (Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine) ;
  • Aibibula, Y. (Applied Greenstock Science, Faculty of Bioresources, Mie University) ;
  • Hanada, M. (Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine) ;
  • Okamoto, M. (Department of Agro-environmental Science, Obihiro University of Agriculture and Veterinary Medicine)
  • Received : 2006.03.13
  • Accepted : 2006.06.26
  • Published : 2007.02.01

Abstract

A $3{\times}3$ factorial design experiment was conducted to investigate the effect of temperature and duration of storage on the fermentation quality of potato pulp ensiled with two fungal inoculants under laboratory conditions. The inoculants, Rhizopus oryzae (R) and Amylomyces rouxii (A) were each added to potato pulp material to contain at least $1{\times}10^6$ CFU/g fresh matter, and silages without additives served as controls. The silages were stored under three temperature regimes; 4, 12 and $25^{\circ}C$. Three silos per treatment from every temperature regime were opened on days 7, 24 and 40 days after ensiling to investigate treatment effects on fermentation quality, starch and sugar concentrations. Increase in temperature and duration of storage had a positive significant effect (p<0.01) on the fermentation quality of potato pulp silage (PPS). The inoculants had little effect (p>0.05) on the fermentation quality of the silages. Sugar concentration in the silages decreased with increase in temperature (p<0.01) but increased (p<0.05) with progression of duration of storage. The fungal inoculants had no effect on starch degradation in PPS. The results suggest that storage temperature and duration of storage are more important in determining the rate of fermentation than addition of the fungal inoculants in PPS.

Keywords

Amylomyces Rouxii;Potato Pulp;Rhizopus Oryzae;Temperature;Duration of Storage

References

  1. De Man, J. C. 1957. The fermentation of cell wall substances in grass silage and in potato pulp. Antonie van Leeuwenhoek. 23:87-96. https://doi.org/10.1007/BF02545861
  2. Mayer, F. and J. O. Hillebrandt. 1997. Potato pulp: Microbial characterization, physical modification, and application of this agricultural waste product. Appl. Microbiol. Biotechnol. 48:435-440. https://doi.org/10.1007/s002530051076
  3. Nguyen, H. V., M. Kawai, J. Takahashi and S. Matsouka. 2004. Change in nitrogen fractions and ruminal nitrogen degradability of orchardgrass and alfalfa during the ensiling process and the subsequent effects on nitrogen utilization by sheep. Asian-Aust. J. Anim. Sci. 17(11):1524-1528. https://doi.org/10.5713/ajas.2004.1524
  4. Oda, Y., K. Saito, H. Yamauchi and M. Mori. 2002. Lactic acid fermentation of potato pulp by the fungus Rhizopus oryzae. Current Microbiol. 45:1-4. https://doi.org/10.1007/s00284-001-0048-y
  5. Saito, K., T. Noda, S. Tsuda, M. Mori, Y. Hasa, H. Kito and Y. Oda. 2006. Effects of the dates of extraction on the quality of potato pulp. Bioresc. Technol. 97(18): 2470-2473. https://doi.org/10.1016/j.biortech.2005.10.022
  6. Skory, C. D. 2000. Isolation and Expression of Lactate Dehydrogenase genes from Rhizopus oryzae. Appl. Environ. Microbiol. 66:2343-2348. https://doi.org/10.1128/AEM.66.6.2343-2348.2000
  7. Hachmeister, K. A. and D. Y. C. Fung. 1993. Tempeh: A moldmodified indigenous fermented food made from soybeans and/or cereal grains. Crit. Rev. Microbiol. 19:137-188. https://doi.org/10.3109/10408419309113527
  8. Abe, A., I. Sujaya, T. Sone, K. Asana and Y. Oda. 2004. Microflora and selected metabolites of potato pulp fermented with an Indonesian starter Ragi Tape. Food Technol. Biotechnol. 42:169-173.
  9. Okine, A., M. Hanada, Y.Aibibula and M. Okamoto. 2005. Ensiling of potato pulp with or without bacterial inoculants and its effect on fermentation quality, nutrient composition and nutritive value. Anim. Feed Sci. Tech. 121:329-343. https://doi.org/10.1016/j.anifeedsci.2005.02.032
  10. Erdogan, E. H. and S. A. Mahinur. 2001. RT-PCR amplification of a Rhizopus oryzae lactate dehydrogenase gene fragment. Enzyme Microbial Technol. 28:259-264. https://doi.org/10.1016/S0141-0229(00)00319-7
  11. Gordon, F. J. 1989. An evaluation through lactating cattle of a bacterial inoculant as an additive for grass silage. Grass Forage Sci. 44:169-179. https://doi.org/10.1111/j.1365-2494.1989.tb01924.x
  12. SAS Institute Inc. 1996. SAS/STAT User's Guide: 6th edn. SAS Institute Inc., Cary, North Carolina.
  13. Guan Wu-tai, G., Ashbell, Y. Hen and Z. W. Weinberg. 2002. The effects of two inoculants applied to forage sorghum at ensiling on silage characteristics. Asian-Aust. J. Anim. Sci. 15(2):218- 221. https://doi.org/10.5713/ajas.2002.218
  14. Baker, S. B. and W. H. Summerson. 1961. The colorimetric determination of lactic acid in biological material. J. Biol. Chem. 38:535-554.
  15. Aibibula, Y., M. Hanada, O. Abdulrazak, S. Murata and M. Okamoto. 2004. Effect of potato pulp supplementation on nitrogen utilization in ruminants. In: Proceedings of the 11 th. Animal Science Congress, Kuala Lumpur, Malaysia. pp. 331-333.
  16. Monteils, V., S. Jurjanz, O. Colin-Schoelien, G. Blanchart and F. Laurent. 2002. Kinetics of ruminal degradation of wheat and potato starches in total mixed rations. J. Anim. Sci. 80:235-241. https://doi.org/10.1093/ansci/80.1.235
  17. Skory, C. D., S. N. Freer and R. J. Bothast. 1998. Production of Llactic acid by Rhizopus oryzae under oxygen limiting conditions. Biotechnol. Lett. 20:191-194. https://doi.org/10.1023/A:1005397028700
  18. Abe, A. 1988. Feed analyses based on carbohydrates and application to the nutritive value of feeds. In: Feed Quality Evaluation Guidebook 2001. Japan Grassland, Livestock and Seed Association. pp. 16-18.
  19. Abe, A., T. Sone, I. N. Sujaya, K. Saito, Y. Oda, K. Asano and F. Tomita. 2003. rDNA ITS sequence of Rhizopus oryzae: Its application to classification and identification of lactic acid producers. Biosc. Biotechnol. Biochem. 67(8):1725-1731. https://doi.org/10.1271/bbb.67.1725
  20. Meeske, R., H. M. Basson and C. W. Cruywagen. 1999. The effect of lactic acid bacteria with enzymes on the fermentation dynamics, intake and digestibility of Digitaria eriantha silage. Anim. Feed Sci. Tech. 81:237-248.
  21. Ashbell, G., T. Kipnis, M. Titterton, Y. Hen, A. Azrieli and Z. G. Weinberg. 2001. Examination of a technology for silage making in plastic bags. Anim. Feed Sci. Tech. 91:213-222. https://doi.org/10.1016/S0377-8401(01)00239-5
  22. McDonald, P., A. R. Henderson and S. J. E Heron. 1991. Microorganisms. In: The Biochemistry of Silage 2 nd edition. Chalcome Publicatons, Aberystwyth. pp. 81-152.

Cited by

  1. Ensiling characteristics of rice whole stillage inoculated with or without Amylomyces rouxii and evaluation of the feeding value for growing Japanese black steers vol.54, pp.10, 2014, https://doi.org/10.1071/AN14058
  2. Feeding value and fermentation characteristics of ensiled sugar beet wet distillers for fattening Holstein steers vol.57, pp.4, 2014, https://doi.org/10.1080/00288233.2014.930049