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Identification and Functional Analysis of LsMNPV Anti-apoptosis Genes

  • Kim, Yu-Sin (College of Life Science, KIM IL SUNG University) ;
  • Xiao, Hua-Zhong (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Du, En-Qi (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Cai, Guo-Shuai (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Lu, Song-Ya (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Qi, Yi-Peng (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
  • Published : 2007.07.31

Abstract

Three anti-apoptosis genes, Ls-iap2, iap3 and p49 were found in Leucania separata multiple nuclear polyhedrovirus. Amino acid sequence homology of Ls-IAP2 and Ls-IAP3 with Op-IAP2 and Op-IAP3 from Orgyia pseddotsugata MNPV were 20% and 42%, while that of Ls-P49 is 28% with Sl-P49 from Spodoptera littorolis MNPV. Ls-IAP2 contains one baculoviral IAP repeat (BIR) domain followed by a RING domain, while Ls-IAP3 contains two BIRs and a RING. Ls-P49 contains a reactive site loop, predicted cleavage site (KKLD$^{74}{\downarrow}$G) that is different from Sl-P49 (TVID$^{94}{\downarrow}$G). Expressed Ls-iap3 or Ls-p49 under presence of actinomycin D in SF9 cells, DNA ladder assayrevealed that Ls- IAP3 or Ls-P49 could block the apoptosis of SF9 cells induced by actinomycin D. Replication of p35 deficient-mutant Autographa californica MNPV in SF9 cells was also rescued when Ls-iap3 or Ls-p49 was expressed transiently. No anti-apoptotic activity was observed for Ls-IAP2. The results showed that both of Ls-IAP3 and Ls-P49 were functional apoptotic suppressors in SF9 cells.

Keywords

Anti-apoptosis;LsMNPV;Ls-IAP;Ls-p49

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