Protein Kinase C-delta Stimulates Haptoglobin Secretion

  • Oh, Mi-Kyung (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Park, Seon-Joo (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Kim, Nam-Hoon (Department of Natural Sciences, College of Medicine, The Catholic University of Korea) ;
  • Kim, In-Sook (Department of Natural Sciences, College of Medicine, The Catholic University of Korea)
  • Published : 2007.01.31


Haptoglobin (Hp) is a glycoprotein that is produced by hepatic cells and secreted into the circulation. While studying the physiologic functions of Hp, we found that Hp synthesized in THP-1 monocytic cells was largely retained within cells, although Hp is considered a secretory protein. To investigate the molecular mechanism on Hp secretion in THP-1 cells, in the present study, we examined the effect of protein kinase C (PKC) on Hp secretion. When several inhibitors of PKC isoforms were tested, only Rottlerin, a specific inhibitor of PKC-$\delta$, completely blocked Hp secretion from cells to culture medium. To confirm the role of PKC-$\delta$ in Hp secretion, Hp-overexpressing COS7 cells were transiently transfected with a wild-type or a dominantnegative mutant of the PKC-$\delta$ gene. Mutant PKC-$\delta$ significantly inhibited Hp secretion, whereas the wild-type gene slightly increased Hp secretion. These results demonstrate that the PKC-$\delta$ signal is involved in Hp secretion.


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