Sensitive determination of paroxetine in canine plasma by liquid chromatography-tandem mass spectrometry (LC-MS/MS)

LC-MS/MS를 이용한 비글견 혈장 중 파록세틴의 고감도 분석

  • Chang, Kyu Young (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.) ;
  • Kang, Seung Woo (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.) ;
  • Han, Sang Beom (College of Pharmacy, Chung-Ang University) ;
  • Youm, Jeong-Rok (College of Pharmacy, Chung-Ang University) ;
  • Lee, Kyung Ryul (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.) ;
  • Lee, Hee Joo (Dept. of Drug Development Supporting Service. BioCore Co., Ltd.)
  • 장규영 (바이오코아(주) 신약개발지원사업부) ;
  • 강승우 (바이오코아(주) 신약개발지원사업부) ;
  • 한상범 (중앙대학교 약학대학) ;
  • 염정록 (중앙대학교 약학대학) ;
  • 이경률 (바이오코아(주) 신약개발지원사업부) ;
  • 이희주 (바이오코아(주) 신약개발지원사업부)
  • Received : 2006.11.07
  • Accepted : 2007.02.27
  • Published : 2007.04.28


A simple and sensitive method for the determination of paroxetine in canine plasma was developed and validated by liquid-liquid extraction and liquid chromatography-tandem mass spectrometry (LC-/MS/MS). Fluoxetine was used as an internal standard. Paroxetine and internal standard in plasma samples were extracted using TBME (tert-butyl methyl ether). A centrifuged upper layer was then evaporated and reconstituted with mobile phase of 50% acetonitrile adjusted to pH 3 by formic acid. The reconstituted samples were injected into a Capcell Pak UG120 ($2.0{\times}150mm$, $5{\mu}m$) column. Using MS/MS with SRM (selective reaction monitoring) mode, the transitions (precursor to product) monitored were m/z $330{\rightarrow}192$ for paroxetine, and m/z $310{\rightarrow}148$ for internal standard. Linear detection responses were obtained for paroxetine concentration range of 0.02~5 ng/mL. A correlation coefficient of linear regression ($R^2$) was 0.9993. Detection of paroxetine in canine plasma was accurate and precise, with limit of quantification at 0.02 ng/mL. The method has been successfully applied to pharmacokinetic study of paroxetine in healthy beagle dogs.


Paroxetine;LC-MS/MS;canine plasma;pharmacokinetic study


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