Comparative analysis of glycerin in cosmetics by LC/MS and 1H NMR

LC/MS와 1H NMR을 이용한 화장품속의 글리세린 비교분석

  • Park, Gyo-Beom (Center for Chemical Analysis, Korea Research Institute of Chemical Technology) ;
  • Park, Chan Jo (Center for Chemical Analysis, Korea Research Institute of Chemical Technology) ;
  • Lee, Sueg-Geun (Center for Chemical Analysis, Korea Research Institute of Chemical Technology)
  • 박교범 (한국화학연구원 화학분석센터) ;
  • 박찬조 (한국화학연구원 화학분석센터) ;
  • 이석근 (한국화학연구원 화학분석센터)
  • Received : 2007.06.12
  • Accepted : 2007.10.08
  • Published : 2007.10.25

Abstract

The comparative analysis of glycerin in cosmetic samples was carried out by LC/MS and $^1H$ NMR spectrometry. For the LC/MS analysis, aqueous solution was controlled in strong basic condition with sodium hydroxide, and benzoyl chloride was added to the solution for the derivatization of glycerin. The derivative was extracted using pentane and analyzed by the LC/MS. For the $^1H$ NMR analysis, sample was directly dissolved in $D_2O$ solvent without pretreatment. The quantitative analysis of glycerin was done by $^1H$ NMR ERETIC method. The analysis results of LC/MS and $^1H$ NMR showed that the calibration curves were a good linearity with $r^2=0.9991$ in the range of 0.1 to $10{\mu}g/mL$ and $r^2=1$ in the range of 25 to $500{\mu}g/mL$, respectively.

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