Effect of Hydraulic Retention Time (HRT) on the Hydrogen Production and Its Dynamic Characteristics in the Anaerobic Digestion Process Using Clostridium beijerinckii Donker 1926

Clostridium beijerinckii Donker 1926을 이용한 혐기성 소화공정에서 체류시간 변화에 의한 수소 생산과 동력학적 특성

  • Jeong, Tae-Young (Division of Environmental Engineering, Yonsei University) ;
  • Cha, Gi-Cheol (Division of Environmental Engineering, Yonsei University) ;
  • Choi, Suk Soon (Department of Biological and Environmental Engineering, Semyung University)
  • 정태영 (연세대학교 환경공학부) ;
  • 차기철 (연세대학교 환경공학부) ;
  • 최석순 (세명대학교 바이오환경공학과)
  • Received : 2007.02.01
  • Accepted : 2007.02.28
  • Published : 2007.04.10


Hydrogen production and its dynamics were investigated in the continuous anaerobic digestion process using Clostridium beijerinckii Donker 1926. In this work, glucose was used as a substrate and hydraulic retention times (HRT) were 0.5, 0.25 or 0.125 day. The removal efficiency of carbohydrate was over 99% under all of HRT conditions. As HRT was shorter, COD removal efficiency became lower while hydrogen content in the total gas and hydrogen production rate became higher. The cell growth yield and hydrogen production yield were 0.27 g-VSS/g-glucose and 0.26 L/g-glucose, respectively, at the steady state. It is expected that the microorganism is able to produce hydrogen when used in the wastewater treatment containing carbohydrate such as glucose. Also, the results in this study could be applied to the actual hydrogen gas production, a promising alternative energy.


  1. T. W. Jeffries, D. R. Omstead, R. R. Cardenas, and H. P. Gregor, Biotech. Bioeng. Symp., 9, 37 (1979)
  2. A. M. Breue and J. G. Andel, Applied Microbiol. Biotech., 20, 40 (1984) https://doi.org/10.1007/BF00254644
  3. T. Fumiaki, J. D. Chang, N. Mizukami, S. T. Tatsuo, and H. Katsushige, Can. J. Microbiol., 39, 726 (1993)
  4. H. Yokoi, T. Ohkawa, J. Hirosse, S. Hayashi, and Y. Takasaki, J. Ferment. Bioeng., 80, 571 (1995)
  5. A. M. Gibson, N. Bratchell, and T. A. Roberts, J. Appl. Bacteriol., 62, 479 (1987) https://doi.org/10.1111/j.1365-2672.1987.tb02680.x
  6. F. Taguchi, J. D. Chang, S. Takiguchi, and M. Morimoto, J. Ferment. Bioeng., 73, 244 (1992) https://doi.org/10.1016/0922-338X(92)90172-Q
  7. P. Perego, B. Fabiano, G. P. Ponzano, and E. Palazzi, Bioproc. Eng., 19, 205 (1998)
  8. J. J. Lay, Y. Y. Li, and T. Noike, J. Environ. Eng., 124, 730 (1998)
  9. APHA, Standard Methods for the Examination of Water and Wastewater, 17th ed., American Public Health Association, Washington, DC (1989)
  10. V. A. Vavilin, S. V. Rytov, and L. Ya. Lokshina, Bioresource Technology, 56, 229 (1996)
  11. K. Naoaki, M Akiko, and K. Koichi, Water Science and Technology, 36, 41 (1997)
  12. S. Ghosh and F. G. Pohland, J. Water Poll. Control Fed., 46, 748 (1974)
  13. P. L. McCarty, Adv. Chem. Ser., 105 (1971)
  14. J. Monod, Ann. Rev. Microbiol., 3, 371 (1949) https://doi.org/10.1146/annurev.mi.03.100149.002103
  15. M. H. Zwietering, I. Jongenburger, F. M. Rombouts, and K. V. Riet, Applied and Environmental Microbiology, 56, 1875 (1990)
  16. A. L. Lehninger, Biochemistry, Worth Publishers Inc., New York (1970)