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Assessment of the Persistence of DNA in Decomposing Leaves of CMVP0-CP Transgenic Chili Pepper in the Field Conditions

포장 조건에서 CMVP0-CP 형질전환 고추 도입유전자의 지속성 조사

  • Lee, Bum-Kyu (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Gi (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Ji-Young (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Kee-Woong (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yi, Hoon-Bok (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Harn, Chee-Hark (Biotechnology Institute, Nongwoo Bio Co. Ltd.) ;
  • Kim, Hwan-Mook (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
  • 이범규 (한국생명공학연구원 바이오평가센터) ;
  • 김창기 (한국생명공학연구원 바이오평가센터) ;
  • 박지영 (한국생명공학연구원 바이오평가센터) ;
  • 박기웅 (한국생명공학연구원 바이오평가센터) ;
  • 이훈복 (한국생명공학연구원 바이오평가센터) ;
  • 한지학 ((주)농우바이오 생명공학연구소) ;
  • 김환묵 (한국생명공학연구원 바이오평가센터)
  • Published : 2007.12.31

Abstract

This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.

Keywords

DNA persistence;chili pepper;gene transfer;soil;transgenic plant

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