Isolation of Multidrug-Resistant Salmonella typhimurium DT104 from Swine in Korea

  • Lee, Ki-Eun (Culture Collection of Antimicrobial Resistant Microbes, Department of Biology) ;
  • Lee, Yeon-Hee (Culture Collection of Antimicrobial Resistant Microbes, Department of Biology)
  • Published : 2007.12.31

Abstract

We report the isolation of Salmonella enterica serotype Typhimurium phage type DT104 (CCARM 8104) from swine in Korea. The CCARM 8104 isolate was resistant to nalidixic acid and showed reduced susceptibility to quinolones. The CCARM 8104 isolate had a missense mutation, Asp87Asn, in the quinolone resistance-determining region in gyrA and produced PSE-1. The CCARM 8104 isolate carried two different class 1 integrons, and the PSE-1 ${\beta}$-lactamase gene was inserted into a 1,200 bp class 1 integron. The presence of DT104 with pse-1 in an integron located in a plasmid and reduced susceptibility to quinolone in swine pose a significant threat of possible horizontal spread between swine and humans.

References

  1. Bolton, L.F., L.C. Kelley, M.D. Lee, P.J. Fedorka-Cray, and J.J. Maurer. 1999. Detection of multidrug-resistant Salmonella enterica serotype Typhimurium DT104 based on a gene which confers cross-resistance to florfenicol and chloramphenicol. J. Clin. Microbiol. 37, 1348-1351
  2. Chung, Y.H., S.Y. Kim, and Y.H. Chang. 2003. Prevalence and antibiotic susceptibility of Salmonella isolated from foods in Korea from 1993 to 2001. J. Food Prot. 66, 1154-1157 https://doi.org/10.4315/0362-028X-66.7.1154
  3. Clinical Laboratory Standards Institute. 2003a. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals, 2nd ed. Approved standard M31-A2. NCCLS, Wayne, Pa, USA
  4. Clinical Laboratory Standards Institute. 2003b. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically- approved standards, 6th ed. NCCLS, Wayne, Pa, USA
  5. Ewin, W.H. 1986. Serologic identification of Salmonella, pp. 201-238. In W.H. Ewing (ed.), Edwards and Ewing's Identification of Enterobacteriaceae, 4th ed. Elsevier Science Publishing Co., Inc., New York, N.Y., USA
  6. Gupta, A., J. Fontana, C. Crowe, B. Bolstorff, A. Stout, S. Van Duyne, M.P. Hoekstra, J.M. Whichard, T.J. Barrett, F.J. Angulo, and The National Antimicrobial Resistance Monitoring System PulseNet Working Group. 2003. Emergence of multidrug- resistant Salmonella enterica serotype Newport infections resistant to expanded-spectrum cephalosporins in the United States. J. Infect. Dis. 188, 1707-1716 https://doi.org/10.1086/379668
  7. Jung, Y., H. Hong, H. Nam, and Y. Lee. 2002. Isolation of norfloxacin resistant Escherichia coli from the Han river and characterization of resistance mechanisim. J. Microbiol. 40, 63-69
  8. Lee, Y.J., K.S. Kim, J.H. Kim, and R.B. Tak. 2004. Salmonella gallinarum gyrA mutations associated with fluoroquinolone resistance. Avian Pathol. 33, 251-257 https://doi.org/10.1080/0301945042000195759
  9. Lee, W.C., M.J. Lee, J.S. Kim, and S.Y. Park. 2001. Foodborne illness outbreaks in Korea and Japan studied retrospectively. J. Food Prot. 64, 899-902 https://doi.org/10.4315/0362-028X-64.6.899
  10. Lee, K., D. Yong, J.H. Yum, H.H. Kim, and Y. Chong. 2003. Diversity of TEM-52 extended-spectrum beta-lactamase-producing non-typhoidal Salmonella isolates in Korea. J. Antimicrob. Chemother. 52, 493-496 https://doi.org/10.1093/jac/dkg385
  11. Leon-Velarde, C.G., H.Y. Cai, C. Larkin, P. Bell-Rogers, R.W. Stevens, and J.A. Odumeru. 2004. Evaluation of methods for the identification of Salmonella enterica serotype Typhimurium DT104 from poultry environmental samples. J. Microbiol. Meth. 58, 79-86 https://doi.org/10.1016/j.mimet.2004.03.005
  12. Levesque, C., L. Piche, C. Larose, and P.H. Rov. 1995. PCR mapping of integrons reveals several novel combinations of resistance genes. Antimicrob. Agents Chemother. 39, 185-191 https://doi.org/10.1128/AAC.39.1.185
  13. Makanera, A., G. Arlet, V. Gautier, and M. Manai. 2003. Molecular epidemiology and characterization of plasmid-encoded $\beta$-lactamase produced by Tunisian clinical isolates of Salmonella enterica serotype Mbandaka resistant to broad-spectrum cephalosporins. J. Clin. Microbiol. 41, 2940-2945 https://doi.org/10.1128/JCM.41.7.2940-2945.2003
  14. Mathew, A., A.M. Harris, M.J. Marshall, and G.W. Ross. 1975. The use of analytical isoelectric focusing for detection and identification of $\beta$-lactamases. J. Gen. Microbiol. 88, 169-178 https://doi.org/10.1099/00221287-88-1-169
  15. Weill, F.X., F. Guesnier, V. Guibert, M. Timinouni, M. Demartin, L. Polomack, and P.A. Grimont. 2006. Multidrug resistance in Salmonella enterica serotype Typhimurium from humans in France (1993 to 2003). J. Clin. Microbiol. 44, 700-708 https://doi.org/10.1128/JCM.44.3.700-708.2006
  16. Yang, S.J., K.Y. Park, S.H. Kim, K.M. No, T.E. Besser, H.S. Yoo, S.H. Kim, B.K. Lee, and Y.H. Park. 2002. Antimicrobial resistance in Salmonella enterica serovars Enteritidis and Typhimurium isolated from animals in Korea: comparison of phenotypic and genotypic resistance characterization. Vet. Microbiol. 24, 295-301