Antifungal activity of pesticides to control dry rot and blue mold during garlic storage

마늘 저장 중 마름썩음병과 푸른곰팡이병 억제를 위한 농약의 살균활성

  • You, Oh-Jong (Department of Crop Life Safety, NIAST, RDA) ;
  • Lee, Yong-Hoon (Plant Pathology Division, NIAST, RDA) ;
  • Jin, Yong-Duk (Department of Crop Life Safety, NIAST, RDA) ;
  • Kim, Jin-Bae (Department of Crop Life Safety, NIAST, RDA) ;
  • Hwang, Se-Gu (Danyang Garlic Experiment Station, Chungbook Agricultural Research and Extension Services) ;
  • Han, Sang-Hyun (Department of Crop Life Safety, NIAST, RDA) ;
  • Kim, Jang-Eok (Division of Applied Biology and Chemistry, Kyungpook National University)
  • 유오종 (농업과학기술원 농산물안전성부) ;
  • 이용훈 (농업과학기술원 농업생물부) ;
  • 진용덕 (농업과학기술원 농산물안전성부) ;
  • 김진배 (농업과학기술원 농산물안전성부) ;
  • 황세구 (충북농업기술원 단양마늘시험장) ;
  • 한상현 (농업과학기술원 농산물안전성부) ;
  • 김장억 (경북대학교)
  • Published : 2007.12.30

Abstract

The major fungal diseases which effecting garlic storage are blue mold and dry rot, caused by Penicillium hirsutum and Fusarium oxysporum, respectively. In order to reduce the damage by the pathogenic fungi, here we report the effects of 11 fungicides tested to reduce spoilage during storage of garlics. In the in vitro antimicrobial activity test, the fungicides, diphenylamine, prochloraz and tebuconazole showed 0.3, 2.2, and 1.3 nun inhibition zone to F. oxysporium, and cyprodinil, diphenylamine, fenbuconazole, hexaconazole, penconazole, prochloraz, propiconazole, pyrimethanil and tebuconazole exhibited 0.2, 2.4, 0.8, 0.4, 1.2, 1.5, 1.2, 0.4 and 1.5 mm to P. hirsutum, respectively. To test the in vivo control effect, when the diphenylamine, prochloraz, and tebuconazole were treated by standard concentration, the fungal mycelium of F. oxysporium started to grow 5 days after inoculation, and 80, 63.3 and 83.3% of the inoculated cloves are infected 11 days after inoculation. When the tebuconazole were treated by standard concentration, the P. hirsutum was completely inhibited the growth of the fungi. In case of diphenylamine, penconazole and propiconazole treatment, the P. hirsutum was observed 7 days after inoculation and $20{\sim}23.3%$ of the cloves were infected 11 days after inoculation. When cyprodinil, prochloraz and pyrimethanil were treated, pathogens occurred 5 days after inoculation and $60{\sim}100%$ of the cloves infected 11 days after inoculation. Three fungicides such as diphenylamine, prochloraz and tebuconazole also suppressed remarkably the infection and growth of F. oxysporium and P. hirsutum on garlic when both of the pathogens are inoculated after the garlic cloves were dipped for 10 min in the suspension of each agrochemical. Overall, diphenylamine, prochloraz and tebuconazole showed effective control efficacy on dry rot and blue mold There was significant correlation between in vitro and in vivo assay in diphenylamine and prochloraz to F. oxysporum and cyprodinil, prochloraz and pyrimethanil to P. hirsutum.

Keywords

Blue mold rot;Dry rot;Fusarium oxysporum;Penicillium hirsutum;Diphenylamine;Prochloraz;pyrimethanil;Tebuconazole;Thiabendazole

References

  1. loos R, A BeIhadj, M. Menez and A. Faure (2005) The effects of fimgicides on Fusarium spp. and Microdochium nivale and their associated trichothecene mycotoxins in french naturally-infected cereal grains. Crop Protection 24:894-902 https://doi.org/10.1016/j.cropro.2005.01.014
  2. Peter L. S., B. Karen and S. Sarah (2005) Sensitivity of Penicillium spp. and Botrytis cinerea to pyrimethanil and its control of blue and gray mold of stored apples. Crop Protection 24: 127-134 https://doi.org/10.1016/j.cropro.2004.07.011
  3. 김용기, 이상범, 이상엽, 심홍식, 최인후 (2003) 마늘 저장병 방제를 위한 경종적, 화학적 접근, 한국농약과학회지 7(2):139-148
  4. 박용문, 황재문, 이병일 (1992) 종구의 저장온도가 마늘의 수확중 품질과 파종후 생육에 미치는 영향. 한국원예과학회지 33(2):103-110
  5. 이승우 (1981) 마늘의 저장성 향상에 고나한 연구. 경북대학교논문집(자연과학) 31:451-458
  6. 김종훈, 김진주, 정진홍, 이호준, 김재능 (2002) 마늘의 줄기 및 뿌리절단에 따른 저장 중 품질변화. 한국식품저장유통학회지 9(4):362-368
  7. 배로나, 윤상돈, 안을균, 목일진, 임채일 (2002) 마늘 종구의 저장온도에 따른 생육 및 구비재의 품종간 차이. 원예과학기술지 20(2):95-99
  8. 조원대, 김완규, 김한명 (1995) 마늘저장병해에 관하여는 진균. 농시논문집 37(2):325-329
  9. Park Y. M., J. M. Hwang and H. T. Ha (2000) Storability of garlic bulbs as influenced by postharvest clipping treatments and storage temperature, J. Kor Soc. Hort. Sci. 41(4):315-318
  10. Koffmann W. and L. J. Penrose (1987) Fungicides for the control of blue mould (Penicillium spp.) in pome fruits. Scientia Horticulturae 31 :225-232 https://doi.org/10.1016/0304-4238(87)90047-1
  11. 고하영 (1983) 마늘 장기저장방법. 전북대학교 석사학위논문
  12. Hwang, J. M. (1988) Effects of temperature and humidity conditions before and after planting on bulb dormancy and development in garlic (Allium sativum L.). Seoul National University
  13. Verniere, C. J., T. R. Gottwald and O. Pruvost, (2003) Disease development and symptom expression of Xanthomonas axonopodis pv. citri in various citrus plant tissues. Phytpathology 9(7):832-843
  14. Chand-Goyal T. and R. A. Spotts (1996) Control of postharvest pear diseases using natural saprophytic yeast colonists and their combination with a low dosage of thiabendazole. Postharvest Biology and Technology 7:51-64 https://doi.org/10.1016/0925-5214(95)00031-3