Comparison of insecticide susceptibility and enzyme activities of biotype B and Q of Bemisia tabaci

담배가루이 Biotype B와 Q의 약제감수성과 효소활성 비교

  • Kim, Eun-Hee (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Sung, Jae-Wook (National Institute of Agricultural Science and Technology, Rural Development Administration) ;
  • Yang, Jeong-Oh (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Ahn, Hee-Geun (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Yoon, Chang-Mann (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Seo, Mi-Ja (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University) ;
  • Kim, Gil-Hah (Dept. of Plant Medicine, Coll. of Agri., Chungbuk National University)
  • 김은희 (충북대학교 농업생명환경대학 식물의학과) ;
  • 성재욱 (농업과학기술원 농약평가과) ;
  • 양정오 (충북대학교 농업생명환경대학 식물의학과) ;
  • 안희근 (충북대학교 농업생명환경대학 식물의학과) ;
  • 윤창만 (충북대학교 농업생명환경대학 식물의학과) ;
  • 서미자 (충북대학교 농업생명환경대학 식물의학과) ;
  • 김길하 (충북대학교 농업생명환경대학 식물의학과)
  • Published : 2007.12.30

Abstract

Mitochondrial 16S DNA sequences of Bemisia tabaci which were collected on rose greenhouse of Iwol and Jinchen in Chungbuk and red pepper field of Miryang, Gyeongnam, were analyzed. The mtCOI PCR product of B. tabaci collected on red pepper field of Miryang were digested with EcoT14I (Sty I) into two fragments 555bp and 311bp, while the PCR product of B. tabaci collected on rose greenhouse of Iwol were digested with Sty I into two fragments of 560bp and 306bp. As a result, B. tabaci collected on red pepper reveal biotype Q and those on rose greenhouse was biotype B. These was difference between two biotypes in insecticide susceptibility, and the biotype B was more susceptible than biotype Q. As a result of foliar systemic test, root-uptake systemic test and residual effect, the biotype B was more susceptible. In case of inhibition effect on enzyme activities of fenitrothion (organophosphorous) and fenothiocarb (carbamate), those of biotype Q was higher than those of biotype B. These results indicate that biotype Q was more resistant than biotype B against 12 insecticides.

Keywords

Bemisia tabaci;DNA sequences;biotype B and Q;insecticide;foliar systemic test;residual effect

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