Differential herbicide response of sulfonylurea-resistant Monochoria vagnalis accessions to sulfonylurea herbicides

서로 다른 지역에서 채집된 Sulfonylurea계 제초제 저항성 물달개비의 제초제 반응 차이

  • Park, Tae-Seon (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Lee, In-Yong (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Park, Jae-Eup (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA) ;
  • Oh, Se-Mun (Department of Agricultural Biology, National Institute of Agricultural Science and Technology, RDA)
  • 박태선 (농업과학기술원 농업생물부 잡초관리과) ;
  • 이인용 (농업과학기술원 농업생물부 잡초관리과) ;
  • 박재읍 (농업과학기술원 농업생물부 잡초관리과) ;
  • 오세문 (농업과학기술원 농업생물부 잡초관리과)
  • Published : 2007.12.30


Four sulfonylurea(SU)-resistant Monochoria vaginalis(M. vaginalis) accessions were tested for levels of resistance to four SU herbicides which have been widely using in paddy fields of Korea, based on whole plant response and sensitivity of the target enzyme, acetolactate synthase(ALS). The resistant Naju, Nonsan and Gimje accessions were not affected to the survival by treatment with recommended dose of all SU herbicides tested. The $GR_{50}$ values for the Naju, Nonsan and Gimje accessions were 8- to 33-fold, 8- to 30-fold and 7- to 32-fold higher to recommended doses of all SU herbicides tested than the susceptible Cheongdo accession, respectively. However, the $GR_{50}$ values for Kimhae accession displayed an intermediate response and was only 4-to 13-fold more resistant than the susceptible accession. The ALS $I_{50}$ values for the Naju, Nonsan and Gimje accessions were 25- to 66-fold, 9- to 26-fold and 10- to 24-fold higher to recommended doses of all SU herbicides tested than the susceptible Cheongdo accession, respectively. However, the $I_{50}$ value for Kimhae accession was 4- to 9-fold more resistant than the susceptible accession, as determined by $I_{50}$ values of ALS.


acetolactate synthase(ALS);Monochoria vaginalis;Resistance;sulfonylurea;herbicide


  1. Carol, A., M. Smith, D. C. Thill, M. I. Dial, and R. S. Zemetra (1990) Inheritance of sulfonylurea herbicide resistance in Lactua spp. Weed Tech. 4:787-790 https://doi.org/10.1017/S0890037X00026403
  2. Genetic analysis of mutants of Saccharomyces cerevisiae resiatsnt to the herbicide sulfometurn-methyl. Genetics. 109:21-35
  3. Gressel, J. and L. A. Segel (1990) Modelling for the effectiveness of herbicide rotations and mixtures as strategies to delay or preclude resistance. Weed Tech. 4:186-198 https://doi.org/10.1017/S0890037X00025215
  4. Heap, I. (2007) International Survey of Herbicide Resistant Weeds
  5. Koarai, A. (2000) Diagnosis of susceptibility of sulfonylurea herbicides on Monochoria vaginalis, an annual paddy weed in Japan. J. Weed Sci. & Tech. 45 suppl.(1):40-41 https://doi.org/10.3719/weed.45.Supplement_40
  6. Kohara, H., K. Konno and M. Takekawa (1999) Occurrence of sulfonylurea-resistant biotypes of Scripus juncodies Roxb. var. ohwianus T. Koyama in paddy fields of Hokkaido perfecture, Japan. J. Weed Sci. & Tech. 44(3):228-235 https://doi.org/10.3719/weed.44.228
  7. Lowry O. H., N. J. R. Rosebrough, A. L. Farr, and R. J. Randall. (1951). Protein measurement with the Folin phenol reagent. J BioI Chem 193:265-275
  8. Malik, R. K., H. Brown, G. W. Cussans, M. D. Devine, S. O. Duken, C. F. Quintanilla, A. Helweg, R. E. Labrada, M. Landes, P. Kudsk, and J.C. Streibig (1996) Herbicide resistant weed problems in developing world and methods to overcome them. Proceedings of the second international weed control congress, Copenhagen, Demnark, 25-28 June 1996. 1-4:665-673
  9. Park, T. S. (2002) Resistance Mechanism and management of sulfonylurea-resistant Monochoria korsakowii, Degree of doctor of philosophy in Kyungpook national university
  10. Park, T. S. Kang, S. Y and Reu S. K. (2007) DNA sequence characterization of the acetolactate synthase (ALS) of sulfonylirea-resistant and susceptible Monochoria vaginalis. Korean Journal of weed Sci. 27(1):41-48
  11. Yoshida, S., K. Onodera, T. Soeda, Y. Takeda, S. Sasaki and H. Watanabe (1999) Occurrence of Scirpus juncodies subsp. ohwianus, resistant to sulfonylurea herbicides in Miyagi Prefecture. J. Weed Sci. & Tech. 44 suppl., 70-71 https://doi.org/10.3719/weed.44.Suppl_70
  12. Dyer, W. E., P. W. Chee, and P. K. Fay (1993) Rapid germination of sulfonylurea-resistant Kochia scoparia L. accessions is associated with elevated seed levels of branched chain amino acids. Weed Sci. 41:18-22
  13. Westerfield, W. W. (1945) A colorimetric determination of blood acetoin. J Biol Chem 161:495-502
  14. Gerwick, B. C., L. C. Mireles, and R. J. Eilers (1993) Rapid diagnosis of ALS/AHAS-resistant weeds. Weed Tech. 7:519-524 https://doi.org/10.1017/S0890037X00027986
  15. Heap, I. (1997) The occurrence of herbicide-resistant weeds worldwide. Pestic. Sci. 51:235-243 https://doi.org/10.1002/(SICI)1096-9063(199711)51:3<235::AID-PS649>3.0.CO;2-N
  16. Ouistoffoleti, P. J. and P. Westra (1994) Competition effects with mixed stands of wheat and kochia(Kochia scoparia) biotypes resistant and susceptible to acetolactate synthase inhibitor herbicides. Scientia Agricola. 51:245-251 https://doi.org/10.1590/S0103-90161994000200008
  17. Ray, R. B. 1984. Site of action chlorsulfuron. Plant Physiol. 75:828-831
  18. Wang, G. X., H. Kohara and K. Itoh (1997) Sulfonylurea resistance in a biotype of Monochoria korsakowii, an annual paddy weed in Japan. Brighton Crop Protection Conference-Weeds 1:311-318
  19. Park, T. S., C. S. Kim, J. P. Park, Y. K. Oh. and K. U. Kim (1999) Resistant biotype of Monochoria korsakowii against sulfonylurea herbicides in the reclaimed paddy fields in Korea. Proc. 17th APWSS Conf.251-254, Bangkok
  20. Van. O., and P. H. Van Leeuwen (1992) Use of fluorescence induction to diagnose resistance of Alopecurus myosuroides Huds.(black-grass) to chlortoluron. Weed Res. 1:19-31
  21. Itoh, K. and K. Ito (1994) Weed ecology and its control in south-east tropical countries. Japanese Journal of Tropical Agriculture. 38:369-373
  22. Heap, I. 2002. International Survey of Herbicide Resistant. Weeds. http://www.weedscience.org/in.asp
  23. Hensley, J. R. (1981) A method for identification of triazine resistant and susceptible biotypes of several weeds. Weed Sci. 29:70-73
  24. Itoh, K., G. X. Wang, H. Shibaike, and K. Matsuo (1999) Habitat management and inhertance of sulfonylurea resistance in Lindernia micratha, an annual paddy weed in Japan. Proc. 17th APWSS Conf. 537-543, Bangkok
  25. Cavan, G. and S. Moss R (1997) Herbicide resistance and gene flow in black-grass(Alopecurus myosuroides) and wild-oats (Avena spp.). BCPC-Weeds. 1:305-310
  26. Donald, L. W. (1992) Future impact of crops with modified herbicide resistance. Weed Tech. 6:665-668 https://doi.org/10.1017/S0890037X00036009