Toxicity Test of butachlor to Medaka

뷰타클로르의 송사리에 대한 독성시험

  • Park, Yeon-Ki (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Kim, Byung-Seok (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Shin, Jin-Sup (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Bae, Chul-Han (Agricultural Research Center, Hankooksamgong Co., Ltd.) ;
  • Park, Kyung-Hun (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Lee, Jea-Bong (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Hong, Soon-Sung (Pesticide Safety Division, National Institute of Agricultural Science and Technology) ;
  • Cho, Kyung-Won (Agricultural Research Center, Hankooksamgong Co., Ltd.) ;
  • Lee, Kyu-Seung (Chungnam National University)
  • 박연기 (농업과학기술원 농산물안전성부) ;
  • 김병석 (농업과학기술원 농산물안전성부) ;
  • 신진섭 (농업과학기술원 농산물안전성부) ;
  • 배철한 (한국삼공(주) 농업연구소) ;
  • 박경훈 (농업과학기술원 농산물안전성부) ;
  • 이제봉 (농업과학기술원 농산물안전성부) ;
  • 홍순성 (농업과학기술원 농산물안전성부) ;
  • 조경원 (한국삼공(주) 농업연구소) ;
  • 이규승 (충남대학교 농업생명과학대학)
  • Published : 2007.12.30

Abstract

In order to evaluate the toxic effects of butachlor, a herbicide widely used for control of weeds in paddy field, on medaka (Oryzias latipes), acute toxicity tests for five developmental stages and early life stage toxicity test of were conducted. As the results of acute toxicity test, $96h-LC_{50}s$ for 1 day, 1 week, 2 weeks, 2 months and 5 months after hatching of O. latipes were 0.68, 0.52, 0.38, 1.09 and $0.45\;mg\;L^{-1}$, respectively. This indicated that the most sensitive stage was 2 weeks after hatching. The early life stage toxicity test showed that no statistically significant hatching period and hatching success of embryo was observed at all concentrations of butaclor. However, 0.05 and $0.1\;mg\;L^{-1}$ of butachlor showed statistically significant post hatching survival with p<0.1. Abnormalities of larva were 2.1, 2.3 and 10% at 0.025, 0.05 and $0.1\;mg\;L^{-1}$ of concentration, respectively. They showed abnormal vertebral axis, craniofacial alteration and retarded yolk-sac resorption. The total length and weight were decreased depending on butachlor concentration the end of test. Weight of larva was showed more sensitive toxic indicator than total length. The toxicological responses of O. latipes to butachlor expressed as LOEC(lowest observed effect concentration), NOEC(no observed effect concentration) and MATC(maximum acceptable toxicant concentration) values were 0.025, 0.013 and $0.018\;mg\;L^{-1}$, respectively.

Keywords

Oryzias latipes;Medaka;Acute Toxicity Test;Early Life-Stage Toxicity Test;Butachlor

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