The Effects of Rat's Sperm Bioassay for Low Dose X-Ray

저선량 X-선 조사가 쥐의 정자에 미치는 생물학적효과

  • Published : 2007.12.30

Abstract

In order to investigate the enhancement effects of low dose radiation on biological activation, this study applied low dose X-ray to the whole body of male rats to find out whether hormesis is induced in male germ cells. Total 36 Sprague-Dawley(SD) rats as experimental animal were subdivided into 6 groups(in 6 rats per group) such as control, 10 mGy, 20 mGy, 50 mGy, 100 mGy and 200 mGy radiation group All the groups showed slightly increasing number of sperms per 0.1g semen ($14.216{\times}10^6,\;13.901{\times}10^6,\;14.153{\times}10^6,\;13.831{\times}10^6,\;14.137{\times}10^6,\;14.677{\times}10^6$ respectively), and the motility of sperms amounted to 50.9%, 49.5%, 55.1%, 54.3%, 48.0% and 52.2% respectively. Particularly, compared to the control, the other 5 groups showed higher male hormone level, and the microscopic observations of testicle tissues showed no vacuolization in seminiferous tubules and testis cells. In the results of this experiment, no harmful effect was observed on Sprague-Dawley (SD) rats for which the dose of radiation was controlled as regulated legally by the Ministry of Science and Technology and the Ministry of Health and Welfare. However, as these results were obtained from a limited number of animals, we cannot maintain that the same effect will be observed in the human body. Therefore, there should be further research on the effect on other animals and ultimately on the human body.

References

  1. Meistrich ML. Efects of Chemotherapy and Radiotherapy on Spermatogenesis. Eur Urol. 1993;23(1):136-141 https://doi.org/10.1159/000474582
  2. 변명우, 이기훈, 박미선, 이철호, 현병화, 김무강, 정영길, 이강이, 이경열, 김종섭, 천기정. 방사선조사 후 Mongolian Gerbil의 고환에서 정자생성에 관한 연구. 한국실험동물학회지 2001;17(1):27-33
  3. 이상석, 박영선, 김흥태, 고성진. 방사선 생물학. 정문각. 2005;211-217
  4. 천기정, 서원숙, 손화영. 중성자 조사후 Black mouse의 고 환 조직 및 정자수 변화에 관한 연구. 방사선방어학회지 2006;31(1):31-35
  5. United Nations Scientific Committee on the Effects of Atomic Radiation. Ionizing radiation: Sources and biological effects. New York: United Nations. 1982;425-569
  6. JUAN GA. Nurture vs Nature: How Can We Optimize Sperm Quality? Journal of Andrology 2003;24(5):640-648 https://doi.org/10.1002/j.1939-4640.2003.tb02719.x
  7. Joos H, Sedlmayer F, Gomahr A, Rahim HBK, Frick J, Kogelnik HD and Rettenbacher L. Endocrine Profiles after Radiotherapy in Stage I Seminoma: Impact of Two Different Radiation Treatment Modalities. Radiother Oncol. 1997;43:159 162
  8. Brown SO. Effects of Continuous Low Intensity Radiation on Successive Generations of the Albino Rat. Genetics 1964;50:1101-1113
  9. 김성호, 이종환, 김세라, 이해준, 이윤실, 김태환, 류시원, 조성기, 안미영. ICR 마우스에서 방사선 용량에 따른 혈액 변화의 관찰. 대한수의학회지 2002;42(2):183-190
  10. Muller WU, Streffer C. Biological Indicators for Radiation Damage. International Journal of Radiation Biology 1991;59(4):863-873 https://doi.org/10.1080/09553009114550771
  11. Fliedner TM, Nothdurft W, and Steinbach KH. Blood cell changes after radiation exposure as an indicator for hemopoietic stem cell function. Bone Marrow Transplant 1988;3:77-84