Enhancement of Transient Gene Expression in Turnip Seedlings by Alkali Injury

알칼리 상해에 의한 순무 유묘에서의 일시유전자 발현 증대

  • Shin, Dong-Il (Department of Biotechnology, Catholic University of Daegu) ;
  • Park, Hee-Sung (Department of Biotechnology, Catholic University of Daegu)
  • 신동일 (대구가톨릭대학교 생명공학과) ;
  • 박희성 (대구가톨릭대학교 생명공학과)
  • Published : 2007.12.31



  1. De Block, M., De Brower, D. and Tenning, P. (1989) Transformation of Brassica napus and Brassica oleacea using Agrobacterium tumefaciens and the expression of the bar and neo genes in the transgenic plants. Plant Physiol. 91, 694-701 https://doi.org/10.1104/pp.91.2.694
  2. Christey, M. C. and Sinclair, B. K. (1992) Regeneration of transgenic kale (Brassica oleracea var. acephala), rape (B. napus) and turnip (B. campestris var. rapifera) plants via Agrobacterium rhizogenes mediated transformation. Plant Sci. 87, 161-169 https://doi.org/10.1016/0168-9452(92)90147-E
  3. Vaquero, C., Sack, M., Chandler, J., Drossard, J., Schuster, F., Monecke, M., Schillberg, S. and Fisher, R. (1999) Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc. Natl. Acad. Sci. USA 96, 11128-11133
  4. Bechtold, N., Ellis, J. and Pelletier, G. (1993) In planta Agrobacterium mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. CR Acad. Sci. Paris. 316, 11941199
  5. Alibert, B., Lucas, O., Gall, L. V., Kallerhoff, J. and Alibert, G. (1999) Pectolytic enzyme treatment of sunflower explants prior to wounding and 동시배양 with Agrobacterium tumefaciens, enhances efficiency of transient $\beta$-glucuronidase expression. Physiol. Plant 106, 232-237 https://doi.org/10.1034/j.1399-3054.1999.106213.x
  6. Floros J. D., Wetzstein, H. Y. and Chinnan, M. S. (1987) Chemical (NaOH) peeling as viewed by scanning electron microscopy: pimiento peppers as a case study. J. Food Sci. 2, 1312-1316
  7. Jefferson, R. A. (1987) Assaying chimeric genes in plants: the gus gene fusion system. Plant Mol. Biol. Rep. 5, 387-405 https://doi.org/10.1007/BF02667740
  8. Knutzon, D. S., Thompson, G. A., Radke, S. E., Jhonson, W. B., Knauf, V. C. and Kridl, J. C. (1992) Modification of Brassica seed oil by antisense expression of a stearoyl-acyl carries protein desaturase gene. Proc. Natl. Acad. Sci. USA 89, 2624-2628
  9. Flores Solis, J., Mlejnek, I. P., Studena, K. and Prochazka, S. (2003) Application of sonication-assisted Agrobacteriummediated transformation in Chenopodium rubrum L. Plant Soil Environ. 49, 255-260 https://doi.org/10.1080/00380768.2003.10410005
  10. Andrews, L. B. and Curtis, W. R. (2005) Comparison of transient protein expression in tobacco leaves and plant suspension culture. Biotechnol. Prog. 21, 946-952 https://doi.org/10.1021/bp049569k
  11. Shin, D. I. and Park, H. S. (2005) Transient expression in Chinese cabbage by hydrogen peroxide-aided agroinfiltration. Agric. Chem. Biotech. 48, 229-230
  12. Negrouk, V., Eisner, G., Lee, H. I., Han, K., Taylor, D. and Wong, H. C. (2005) Highly efficient transient expression of functional recombinant antibodies in lettuce. Plant Sci. 169, 433-438 https://doi.org/10.1016/j.plantsci.2005.03.031
  13. Jun, S. I., Kwon, S. Y., Paek, K. Y. and Paek, K. H. (1995) Agrobacterium-mediated transformation and regeneration of fertile transgenic plants of chinese cabbage (Brassica campestris ssp. pekinensis cv 'Spring flavor'). Plant Cell Rep. 14, 620-625 https://doi.org/10.1007/BF00232725
  14. Kim, S. S., Shin, D. I. and Park, H. S. (2007) Transient $\beta$- glucuronidase expression in lily (Lilium longiflorum L.) pollen via wounding-assisted Agrobacterium-mediated transformation. Biotech. Lett. 29, 965-969 https://doi.org/10.1007/s10529-007-9326-5
  15. Shin, D. I. and Park, H. S. (2005) Hydrogen peroxide effect on Agrobacterium-mediated alfalfa sprouts transformation. Agric. Chem. Biotechnol. 48, 226-228
  16. Mukhopadhyay, A., Armugam, N., Nandakumar, P. B. A., Pradhan, A. K., Gupta, V. and Pental, D. (1992) Agrobacterium-mediated transformation of oil seed Brassica campestris: transformation frequency is strongly influenced by the mode of shoot regeneration. Plant Cell Rep. 11, 506-513
  17. Doran, P. M. (2000) Foreign protein production in plant tissue cultures. Curr. Opin. Biotech. 11, 199-204 https://doi.org/10.1016/S0958-1669(00)00086-0
  18. Fisher, R., Vaquero-Martin, C., Sack, M., Drossard, J., Emans, N. and Commandeur, U. (1999) Towards molecular farming in the future: transient protein expression in plants. Biotechnol. Appl. Biochem. 30, 113-116
  19. Giddings, G. (2001) Transgenic plants as protein factories. Curr. Opin. Biotech. 12, 450-454 https://doi.org/10.1016/S0958-1669(00)00244-5