Antioxidant Activity of Isolated Compounds from the Heartwoods of Rhus verniciflua

옻나무(Rhus verniciflua) 목질부에서 분리한 화합물의 항산화활성

  • Ahn, Eun-Mi (Department of Herbal Foodceutical, Daegu Haany University) ;
  • Park, Sang-Jae (AZI Co. Ltd.) ;
  • Choi, Won-Cheol (Cancer Center, East-West Neo Medical Center, KyungHee University) ;
  • Choi, Suk-Hoon (Cranbrook 12 grade Michigan) ;
  • Baek, Nam-In (Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
  • 안은미 (대구한의대학교 한방식품약리학과) ;
  • 박상재 ((주)에이지) ;
  • 최원철 (경희대학교 동서신의학병원 암센터) ;
  • 최석훈 (미시건 대학) ;
  • 백남인 (경희대학교 생명공학원 식물대사연구센터)
  • Published : 2007.12.31

Abstract

The heartwoods of Rhus verniciflua was extracted with $H_2O$ and the concentrated extract was partitioned with $CHCl_3$, EtOAc, n-BuOH and $H_2O$, successively. From the EtOAc and n-BuOH fractions, four compounds were isolated through the repeated silica gel, ODS and Sephadex LH-20 column chromatographies. They were determined as sinapyl aldehyde (1), 2,4-dihydroxybenzaldehyde (2), 2,4-dihydroxybenzoic acid (3) and 2,4-dihydroxyphenylglyoxylic acid (4) on the basis of spectroscopic data, respectively. Among the isolated compounds, sinapyl aldehyde $(34.7{\pm}0.6%)$ and 2,4-dihydroxyphenylglyoxylic acid $(43.8{\pm}0.9%)$ showed the strong antioxidative activity than artificial antioxidant BHT $(14.4{\pm}0.3%)$ in DPPH radical scavenging activity.

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