The Antimicrobial Activity against Helicobacter pylori and Antioxidant effect from the extracts of Mulberry leaves (Morus Alba L.)

품종이 다른 뽕잎추출물의 Helicobacter Pylori에 대한 항균활성 탐색과 항산화 효과

  • Cho, Young-Je (Department of Food Engineering, Sangju National University) ;
  • Ju, In-Sik (Department of Food Engineering, Sangju National University) ;
  • Kim, Byung-Oh (Department of Applied Biology, Sangju National University) ;
  • Kim, Jeung-Hoan (NIP Biotech.) ;
  • Lee, Byung-Gu (Department of Culinary & Bakery, Yuengnam Foreign Language College) ;
  • An, Bong-Jeun (Department of Cosmeceutical Science, Daegu Haany University) ;
  • Choo, Jae-Weon (Sericulture & Entomology Experiment Station, Gyeongbuk Agricultural Technology Administration)
  • 조영제 (상주대학교 식품공학과) ;
  • 주인식 (상주대학교 식품공학과) ;
  • 김병오 (상주대학교 생물응용학과) ;
  • 김정환 ;
  • 이병구 (영남외국어대학 호텔조리제빵과) ;
  • 안봉전 (대구한의대학교 화장품약리학과) ;
  • 추재원 (경상북도농업기술원 잠사곤충사업장)
  • Published : 2007.12.31


The water extracts and 80% ethanol extracts from mulberry leaves of 108 kinds were tested their antimicrobial activities against Helicobacter pylori and antioxidant effects. The ABTS [2,2'-azino-bis(3-ethylbenzothiaznoline-6-sulfornic acid)] radical decolorization, electron donating ability (EDA), thiobarbituric acid reactive substance (TBARS) and antioxidant protection factor (PF) were determined for water extracts and 80% ethanol extracts from mulberry leaves. In the electron donating activity, the 13 kinds of water extracts showed high inhibition (>80%), whereas the 59 kinds of 80% ethanol extracts showed high inhibition. The inhibitory activities of water extracts from all kinds of mulberry leaves were higher than 90% in ABTS [2,2'-azinobis(3-ethylbenzothiaznoline-6-sulfornic acid)] radical decolorization. The thiobarbituric acid reactive substance (TBARS) and antioxidant protection factor (PF) of the 80% ethanol extracts were higher than that of water extracts. Antimicrobial activity against Helicobacter pylori showed high value in 80% ethanol extracts of 15 kinds mulberry leaves. The results implied that the mulberry leaves can be useful for natural antimicrobial medicine.


  1. Lee, W. C., Kim, A. J. and Kim, S. Y. (2003) The study on the Functional materials and Effects of Mulberry leaf. Food Sci. and Indust. 36, 2-14
  2. Kim, S. Y., Ryu, K. S., Lee, W. C., Ku, H. O., Lee, H. S. and Lee, K. R. (1999) ypoglycemic Effect of Mulberry Leaves with Anaerobic Treatment in Alloxan-induced Diabetic Mice. Kor. J. Pharmacogn. 30, 123-129
  3. Robert, I. L. (1994) In Functional Foods Phytochemicals and antioxidants. Goldberg, I. (ed.) Chapman & Hall, New York. p.393
  4. Lee, S. E., Bang, J. K., Song, J., Seong, N. S., Park, H. W., Chung, H. G., Kim, G. S. and An, T. J. (2004) Inhibitory Activity on Angiotensin Converting Enzyme (ACE) of Korean Medicinal Herbs. Korean J. Medcinal Crop Sci. 12, 73-78
  5. Aruoma, O. I. (1998) Free radical, oxidative stress and antioxidants in human health and disease. J. Am. Oil chem. Soc. 75, 199-212
  6. Shin, D. H. (1996) The trend and direction of natural antioxidants research. Food Sci Ind. 30, 4-21
  7. Kyrtopoulos, S. A. (1989) N-nitroso compound formation in human gastric juice. Cancer Surv. 8, 423-442
  8. Goodwin, C. S. and Worsiey, B. W. (1993) Helicobacter pylori: Biology and clinical ratice. CPC press, Inc. USA
  9. Marshall, B. J. and Warren, J. R. (1984) Unidentified curved bacilli in the stomach of patients with gastric and peptic ulceration. Lancet. 1, 1311-1315
  10. Hirayama, F., Takagi, S., Yokiyama, Y., Iwao, E. and Ikeda, Y. (1996) Establishment of gastric Helicobacter pylori onfection in Mongolian gerbils. J. Gastroenterol. 31, 24-28
  11. Hahm, K. B., Lee, K. M., Kim, Y. B., Han, S. U. and Kim, M. W. (2001) Animal models of Helicobacter pylori infection. Kor. J. Gastroenterol. 37, 399-405
  12. Lee, S. H., Lim, C. Y., Lee, K. H., Yeo, S. J., Kim, B. J., Kim, S. J., Cho, M. J., Rhee, K. H. and Kook, Y. H. (1999) rpoB Gene analysis of Helicobacter pylori. J. Kor. Soc. Microbiol. 34, 401-408
  13. Hunt, R. H. (1996) Eradication of Helicobacter pylori infection. Am. J. Med. 100, 425-505
  14. Satoh, K., Kimura, K., Taniguchi, Y., Yoshida, Y., Kihira, K., Takimoto, T., Kawata, H., Saifuku, K., Ido, K., Takemoto, T., Ota, Y., Tada, M., Karita, M., Sakaki, N. and Hoshihara, Y. (1996) Distribution of inflammation and atrophy in the stomach of Helicobacter pylori.-positive and -negative patients with chronic gastritis. Am. J. Gastroenterol. 91, 963-969
  15. Rauwa, E. A.J., Langenberg, W., Hoythoff, J. H., Zanen, H. C. and tytgt, G. H. J. (1988) Campylobacter pyloridis-associated chronic active antral gastritis: a proseoective study of its prevelance and the effects of anthibacterial and aniyclera prevelance and the effects of aninacterial and antiuclera treatment. Gasreryerol. 94, 33-40
  16. Borody, T., Lene, J. and Moore-Jones, D. (1990) Is doxycycline more effective than eracycline HCL in triple herapy of Helicobacter pylori. Gastroenterol. 98, 24-29
  17. Park, C. K., Choi, H. J., Youn, H. S., Lee, W. K., Cho, N. J., Kang, K. H., Baik, S. C., Rhee, K. H. (1994) Chemotherapy of Helicobacter pylori infection. J. Kor. Soc. Microbiol. 29, 421-435
  18. Kim, K. S., Shim, S. H., Jeong, G. H. and Cheong, C. S. (1998) Antidiabetic activity of constituents of Lycii fructus. J. of Appl. Pharmacol. 6, 378-382
  19. Pellegrin, N., Roberta, R., Min, Y. and Catherine, R. E. (1998) Screening of dietary carotenoids and carotenoid-rich fruit extracts for antioxidant activites applying 2,2'-azinobis(3-ethylenebenzothiazoline-6-sulfonic acid) radical cation decolorization assay. Method Enzymol. 299, 379-389
  20. Blois, M. S. (1958) Antioxidant determination by the use of stable free radical. Nature 26, 1198-1199
  21. Andarwulan, N. and Shetty, K. (1999) Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium transformed roots of anise (pimpinella anisum L.). J. Agric. Food Chem. 47, 1776-1780
  22. Buege, J. A. and Aust, S. D. (1978) Microsomal lipid peroxidation. Method Enzymol. 105, 302-310
  23. Gavidson, P. H. and Parish, M. E. (1989) Methods for testing the efficacy of food antimicrobals. Food Technol. 43, 148-150
  24. Kim, J. H., Yoon, S. J., Cho, Y. J. (2005) Antimicrobial Activity against Helicobacter pylori and Antioxidant Activity of Yerusalem sage (phlomis frutcosa L.). J. Kor. Soc. Appl. Biol. Chem. 48, 178-182
  25. Hertong, M. C. L., Feskens, E. J. M., Hollman, P. C. H., Katan, M. B. and Kromhout, D. (1993) Dietary antioxidant flavonoids and risk of coronary heart disease. Lancet. 342, 1007-1011
  26. An, B. J., Lee, J. T., Lee, S. A., Kwak, J. H., Park, J. M., Lee, J. Y. and Son, J. H. (2004) Antioxidant effects and applocation as natural ingredients of Korean sanguisorbae officinalis. J. Kor. Soc. Appl. Biol. Chem. 47, 244-250
  27. Kono, Y., Kashone, S., Yoneyama, T., Sakamoto, Y., Matsu, Y. and Shibata, H. (1998) Iron chelation by chlorogenic acid as a natural antioxidant. Biosci. Biotechnol. Biochem. 62, 22-27
  28. Fridovich, I. (1986) Biological effects of the superoxide radical. Arch. Biochem. Biophys. 247, 1-15
  29. Diker, K. S. and Hascelik, G. (1994) The bactericidal activity of tea against Helicobacter pylori. Letters in Appl. Microbiol. 19, 299-300
  30. Cushman, D. W. and Ondetti, M. A. (1980) Inhibitors of angiotensin converting enzyme for treatment of hypertension. Biochem. Pharmacol. 29, 1871-1877