Antioxidant Activities of Extract with Water and Ethanol of Perilla frutescens var. acuta kudo Leaf

차조기(Perilla frutescens var. acuta kudo) 잎의 물과 에탄올 추출물의 항산화 활성

  • Kim, Mi-Hyang (Department of Food Science & Nutrition, Sangju National University) ;
  • Kang, Woo-Won (Department of Food Science & Nutrition, Sangju National University) ;
  • Lee, Nan-Hee (Department of Food Science & Nutrition, Catholic University of Daegu) ;
  • Kwoen, Dae-Jun (Department of Oriental Medicinal Resources, Asia University) ;
  • Choi, Ung-Kyu (Department of Oriental Medicinal Food and Nutrition, Asia University)
  • 김미향 (상주대학교 식품영양학과) ;
  • 강우원 (상주대학교 식품영양학과) ;
  • 이난희 (대구가톨릭대학교 식품영양학과) ;
  • 권대준 (아시아대학교 한약자원학과) ;
  • 최웅규 (아시아대학교 한방식품영양학과)
  • Published : 2007.12.31


This study was conducted to examine antioxidant activities of Perilla frutescens var. acuta leaf. For the this purpose, DPPH radical scavenging activity, lipid oxidation inhibition, SOD-like activity, and xanthine oxidase inhibitor activity of water extract, ethanol extracts (30, 50, 70, and 95%) and the fractions obtained from these extracts were determined. The electron donating abilities of the chloroform fraction obtained from the 70% and 95% ethanol extracts were 50%, and that of the ethyl acetate fraction for all of the extracts was above 75%. In particular, the electron donating ability of the ethyl acetate fraction of the 70% ethanol extract showed the greatest activity with 200.5 ppm of $RC_{50}$ value. The 70% ethanol extract was most effective to inhibit the automatic oxidation of linoleic acid at $40^{\circ}C$ storage. The highest inhibition effects appeared in the chloroform and ethyl acetate fractions of the water extract, and the 30, 50, and 70% ethanol extracts, and the highest lipid oxidation inhibiting effect of the 95% ethanol extract occurred in the hexane and acetate fractions. The SOD-like activity of the water extract was 30.3%, and the activities of the various concentration of ethanol extracts were 28-32% and the activity of the 70% ethanol extract was the highest. The SOD-like activity of the ethyl acetate fraction of the 70% ethanol extract was highest with 1,549.0 ppm of $RC_{50}$ value. Xanthine oxidase inhibition activity was greatest in the water extract and the activities of the ethanol extracts were 36-41.2%. The xanthine oxidase inhibition activity of the ethyl acetate fraction of the water extract was highest. In summary, we found that electron donating ability, lipid oxidation inhibition, and SOD-like activity of Perilla frutescens var. acuta leaf were greatest in the ethyl acetate fraction of the 70% ethanol extract, and xanthine oxidase inhibition activity was highest in the ethyl acetate fraction of the water extract.


  1. Albertazzi, P. and Steel. S. A. (2002) Clifford E & Bottazzi M. Attitudes towards and use of dietary supplementation in a sample of postmenopausal women. Climacteric 5, 374-382
  2. Kedziora, J., and Bartosz, G. (1988) Down's syndrome: a pathway involving the lack of blance of reactive oxygen species. Free Radic. Biol. Med. 4, 317-330
  3. Cross, E. E., Halliwell, B., Borish, E. T., Pryor. W. A., Ames, B. N., Saul, R. L. and McCord, J. M. (1987) Oxygen radicals and human disiease. Ann. Intren. Med. 107, 536-545
  4. Sozmen, E. Y., Tanyakin, T., Onat, T., Kufay, F. and Erlacin, S. (1994) Ethanol-induced oxidative stress and membrane injury in rat erythrocytes. European J. of Clinical Chem. and Clinical Biochem. 32, 741-744
  5. Frei, B. Natural antioxidants in uman and disease. Academic Press. 25-55 p
  6. Mavelli, I., Ciriolo, M. R., Ratilio, G., De S. P., Castorino, M. and Stabile A. (1982) Superoxide dimutase, glutathion peroxidase and catalase in oxidative hemolysis. A study of Fanconi's anaemia erythrocytes. Biochem. Biophys. Res. Comm. 106, 286-290
  7. Blois, M. S., (1958) Antioxidant determination by use of a stable free radical. Nature 26, 1199-1200
  8. Sen, C. K. (1995) Oxidants and antioxidants in exercise. Appl. Physiol. 79, 675-692
  9. Fridorich, I. (1978) The biological activity of oxygen radicals. Science 201, 875-881
  10. Imlay, I. A. and Linn, S. (1986) DNA damage and oxygen radical toxicity. Science 240, 1302-1309
  11. Branen, A. L. (1991) Toxicological and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52, 59-63
  12. Barlow, S. M. (1990) Toxicological aspects of antioxidants used as food additives. In Food Antioxidant. Hudson BJF, ed. Elsevier, Amsterdam. 253-307 p
  13. Eliott, J. G. (1999) Application of antioxidant vitamins in foods and beverages. Food Tech. 53, 46-48
  14. Yamamoto, k. and Niki, E. (1988) Prooxidant effect of $\alpha$- tocopherol. Biochem. Biophys. Acta 958, 19-23
  15. Nam, S. H. and Kang, M. Y. (2000) Screening of antioxidative activity of hot-water extracts from medicinal plants. J. Korean Soc. Agric. Chem. Biotecnol. 43, 141-147
  16. Kim, S. M., Cho, Y.S. and Sung, S. K. (2001) The antioxidant ability and nitrite scavenging ability of plant extracts. Korean J. Food Sci. Technol. 33, 626-632
  17. Yang, S. A., Im, N. K. and Lee, I. S. (2007) Effects of methanolic extract from Salvia miltiorrhiza Bunge on in vitro antithrombotic and antioxidative activities. Korean J. Food Sci. Technol. 39, 83-87
  18. Yoon, G. A. (2006) Effect of garlic supplement and exercise on plasma lipid and antioxidant enzyme system in rats. Korean Food Nutr. Soci. 39, 3-10
  19. Han, S. H., Woo, N. Y., Lee, S. D. and Kang, M. H. (2006) Antioxidative and antibacterial activities of endemic plants extracts in Korea. Korean J. Medicinal Crop Sci. 14, 49-55
  20. Kim, H. Y., Park, Y. K., Kim, T. S. and Kang, M. H. (2006) The effect of green vegetable drink supplementation on cellular DNA damage and antioxidant status of Korean smokers. Korean Food Nutr. Soci. 39, 18-27
  21. Kim, M. H., Lee, M. H., Lee, N. H., Kwon, D. J. and Choi, U. K. (2007) Antimicrobial activity of aqueous ethanol extracts of Perilla frutescens var. acuta leaf. J. of the Kor. Soc. of Food Culture. 22, 182-189
  22. Yoon, Y. S., Lee, S. H., Baek, N. I., Kim, H. Y. and Park, C. H.. (2004) Innibition of Cell Growth and Urease Activity of Helicobacter pylori by Medicinal plant Extracts. Korean J. Biotechnol. Bioeng. 19, 187-191
  23. Lee, E. S. and Seo B. I. (2005) Growth inhibiton of perilla frutescens var. acuta extract. Kor. J. Herbology. 20, 83-89
  24. Okuyama, H. (1992) Minium requirements of n-3 and n-6 essential fatty acids for the function of central nervous system and for the prevention of chronic disease. Proceedings of the Society for Experimental Biology & Medicine 174-176
  25. Nakayama, M., Ju, J. J. R., Sugano, M., Hirose, N., Ueki, T., Doi, F. and Eynard, A. R. (1993) Effect of dietary fat and cholesterol on dimethylbenz. (a)-anthracene-induced mam-mary tumorigenesis in Sprague-Dawley rats. Anticancer Res. 13, 6791-6798
  26. Han, D. S., Kim, Y. I., Choi, K. E., Kwang, J. S. and Baek, S. H. (1998) Development of anticancer agents from Korean medicinal plants. Part 7. Cytotoxic activity of the chloroform soluble fraction of Perilla frutescens against human oral epitheloid carcinoma cells. Environmental Mutagens & Carcinogens. 18, 37-42
  27. Sugaya, A., Tsuda, T. and Obuchi, T. (1981) Pharmacological studies of perillae hera I. neuropharmacological action of water extract and perillaldehyde, Yakugaku Zasshi. 101, 642-648
  28. Kameoka, H. and Nishikawa, K. (1976) The composition of the essential oil from Perilla frutescens L. Brit. var. acuta Thunb. Kudo and Perilla frutescens L. Brit. var. acuta thunb. kudo f. discolor Makino. Nippon Nogeikaku Kaishi. 50, 345-349
  29. Okuda, T., Hatano, Y., Agata, I. and Nishibe, S. (1986) The components of tannic plants in Japan. Yakugaku Zasshi. 106, 1108-1111
  30. Honda, G., Koezuka, Y., Kamisako, W. and Tabata, M. (1986) Isolation of sedative principles from perilla frutescens. Chem. Pharm. Bull. 34, 1672-1677
  31. Fujita, T., Nakayama, M. and Perilloside, A. (1993) A monoterpene glucoside from perilla frutesens. Phytochem. 34, 1545-1548
  32. Fujita, T. and Nakayama, M. (1992) Monoterpene glucoside and other constituents from perilla frutescens. Phytochem. 31, 3265-3267
  33. Aritomi, M., Kamura, T. and Kawasaki, T. (1985) Cyanogenic glycosides in leaves of Perilla frutescens var. acuta. Phytochem. 24, 2438-2439
  34. Yoshida, K., Kameda, K. and Kondo, T. (1993) Diglucanoflavones from purple leaves of Perilla acimoides. Phytochem. 33, 917-919
  35. Tada, M., Matsumoto, R., Yamaguchi, H. and Chiba, K.. (1996) Novel antioxidants isolated from Perilla frutescens Britton var. crispa (Tunb), Biosci. Biotech. 60, 1093-1095
  36. Bios M. S. (1958) Antioxidant determination by the use a stable free radical. Nature 26, 1199-1200
  37. Mitsuda H, Yasumoto K, Iwami K. (1966) Antioxidative action of indol X compounds during the antioxidation of linoleic acid. Eiyo to shokuro. 19, 210-214
  38. Marklund, S. and Marklund, G. (1974) Involvement of superoxide anion radical in the oxidatin of pyrogallol and convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 468-474
  39. Stripe, F. and Della, C. E. (1969) The regulation of rat liver xanthine oxidase. Conversion in vitro of the enzyme activity from dehydrogenase (type D) to oxidase (type O). J. Biol. Chem. 244, 3855-3863
  40. Cho, M. J., Park, M. J. and Lee, H. S. (2007) Nitrite scavenging ability and SOD-like activity of sterol glucoside from Chrysanthemum coronarium L. var. spatiosum. Korean J. Food Sci. Technol. 39, 77-82